Table of Content

    05 January 2012, Volume 32 Issue 1
    FIuorescent in situ hybridization with a panel of probes detects cytogenetic abnormalities in patients with chronic lymphocytic leukemia
    2012, 32(1):  1-6. 
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    【Abstract】objective Firstly to investigate the significance of fluorescence in situ hybridization(FISH) in detecting cytogenetic aberrations in Chinese patients with chronic lymphocytic leukemia(CLL) and secondly explore the characteristics and prognostic significance of cytogenetic aberrations in Chinese patients with CLL.Methods Eighty-four cases of CLL detected by FISH with a panel of probes(CSP12(12p11.1~12q11.1), D13s25(13q14.3),ATM(11q22.3),RB1(13q14), p53(17p13.1)) were retrospectively analyzed by clinical manifestations, laboratory tests and prognosis. Results Cytogenetic aberrations were found in 62 of the 84 CLL patients(73.8%).The most frequent abnormality detected was del(13q14) (56%), followed by del(17p13)(28.6%), del12(16.7%), del(11q22.3)(13.1%), trisomy 12(13.1%). The aberration of 12 deletion was a novel discovery. The trisomy 12 propotion of the group older than 60 was significantly higher than that of the group aged<60(24%VS3%, P=0.042).There was no significant relationship between cytogenetic aberrations and sex, Binet stages, level of lactate dehydrogenase, expression of CD38. The proportion of CD38 expression in LDH elevation group was significantly higher than LDH normal group. (67%VS20%, P=0.015). The median survival time with del(17p13), or del(11q22.3) or complex cytogenetic aberrations was 75 months, the median survival time with sole del(13q14) was 150 months, the median survival time with the others was 120 months, but there was no significant deference with each other. Conclusions FISH is a rapid,accurate and sensitive technique for detection of cytogenetic aberration in CLL. The frequencies of the chromosomal abnormalities in Chinese CLL patients are similar to those in Western countries, but the aberration of 12 deletion was a novel discovery.
    Clinical Analysis of 155 Adrenal Cyst Cases
    2012, 32(1):  7-11. 
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    Objective: To discuss the diagnosis and treatment for adrenal cysts, and evaluate the safety and efficacy of laparoscopic surgery for adrenal cysts. Methods:During the period of 1990-1~2011-7, 155 patients with adrenal cysts underwent surgery in Peking Union Medical College Hospital. The preoperative symptoms, preoperative diagnosis, treatments, intra- and postoperative complications, subclassification of adrenal cysts by Histology, and follow-up in patients after surgery were reviewed respectively. Results: We identified 155 patients who underwent surgery for adrenal cysts, and 62 cases were male, 93 female. The median age is 43 years.154 adrenal cysts were unilateral, and 1case is bilateral. The diameter is 2.6cm on average, ranging from 2 to 11cm.The accuracy of diagnosis of CT, MRI, and B-us is 92.2%, 86.4%, 61.0% respectively. By endocrinology test, 2 cases were diagnosed as primary aldosteronism, 2 cases as pheochromocytoma.146 laparoscopic operations were performed, and 9 open operations were performed. The time of operation for laparoscopy and open procedure is 55min and 153 min on average respectively, while the hospital stays after operation is 2.2days and 8.6 days respectively. No severe perioperative complications occurred. Subclassification of adrenal cysts by histology includes endothelial cysts(86/156), pseudocysts(67/156), and epithelial cysts(3/156). At follow-up, no patient suffered recurrence. Conclusion: Diagnosis of adrenal cysts predominantly depends on imaging, especially enhanced CT. Preoperative endocrinology test is necessary. Surgery is recommended for large cysts greater than 5 cm, cystic endocrine active adrenal tumors and cysts with chances of malignancy by imaging. Laparoscopic surgery should be preferred as the first option for the surgical treatment of adrenal cysts. Keywords:adrenal cysts, diagnosis, treatment ,surgery,laparoscopy
    The expression of miRNA-143 during adipogenic differentiation of MSCs
    2012, 32(1):  12-15. 
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    Objective: To investigate the expression of miR-143 during adipogenic differentiation of Mesechymal stem cells(MSCs)in mice, and the regulation mechanisms of MSCs’ adipogenic differentiation. Methods:The MSCs of C57BL/6 mice were isolated, cultured by using the whole bone marrow method, amplified by the differential adherent method, and adipogenic differentiation of the fifth generation of MSCs was induced with the adipogenic medium. We used the microRNA microarray to explore the expression of miR-143 in the MSCs group and adipocytes group, and validated it by real-time quantitative polymerase chain reaction (real-time PCR). Results:We got high-purity MSCs and Oil O staining showed that lipid droplets were increased in MSCs treated with adipogenic conditions, which implied that the MSCs were induced into adipocyte successfully. The miR-143 was up-regulated during adipogenic differentiation showed by microRNA microarray analysis and confirmed by using real -time PCR(3.73±0.42 vs 1.00±0.14,P<0.01).Conclusion: MiRNA-143 may take part in regulating the adipogenic differentiation of MSCs.
    The regulatory effects of PINK1 on dopamine biosynthesis
    2012, 32(1):  16-20. 
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    Abstract: Objective To explore the role for PINK1 in the regulation of dopamine biosynthesis. Methods The dopaminergic MN9D cell was used as a cell model for the present study. PINK1 gene was knockdown by RNAi technique. The dopamine (DA) level, mRNA and protein expression level of (tyrosine hydroxylase, TH) and (dopa decarboxylase, DDC) were then detected by HPLC with electrochemical detection, real-time RT-PCR or Western blots 48h later, respectively. The association between PINK1 and TH was observed with Co-immunoprecipitation and immunohistochemistry. Results When PINK1 was silenced, DA content was 5.356±0.536ng/ml significantly lower than the control group of 11.63 ± 1.559ng/ml (p<0.05), TH mRNA and protein expression were 0.3713±0.1403 and 0.1956 ±0.06212 significantly lower than that of control group 1.009±0.1528 and 0.3861±0.04455(p<0.05),DDC mRNA and protein expression were 0.3963±0.1241 and 0.1492 ±0.02940 significantly lower than that of control group1.009±0.1528 and 0.3861±0.04455, respectively.(p<0.05) Conclusion PINK1 had a regulatory effect on dopamine biosynthesis, which is caused by its influence on TH and DDC, two important enzymes for DA synthesis.
    Comparision of plasma osteoprotegerin and related factors in two groups of pregnant women
    2012, 32(1):  21-24. 
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    Abstract Objective The aim of the present study was to determine the plasma osteoprotegerin (OPG) levels in pregnant women with gestational diabetes mellitus (GDM) or with normal glucose tolerance (NGT) during their mid-late pregnancy, and to investigate the relationship between OPG and its involved factors. Method In this study, 65 women with GDM and 65 women with NGT were enrolled. Blood samples of each participant were collected during the 24-28 weeks of gestation using the EDTA anticoagulation tube, then centrifuged at 3000 × g for 5 min and collected the plasma. The samples of plasma were stored at -20 ℃ until use. Plasma concentration of OPG was measured by enzyme linked immunosorbent assay (ELISA). We also measured the level of fasting plasma glucose (FPG), fasting insulin (FINS), glycated hemoglobin (HbA1c), lipid, high-sensitivity C-reactive protein (hs-CRP), blood cell count, and calculated the homeostasis model assessment of beta-cell function (HOMA-B) and insulin resistance (HOMA-IR). Results The levels of plasma OPG were not significantly different between women with GDM and NGT. In the group of NGT, OPG levels were positively correlated with FINS (r=0.335, P=0.012),HOMA-IR (r=0.363, P=0.006), platelet count (r=0.333, P=0.009) and negatively related with apolipoprotein B (r=-0.254, P=0.043), but in GDM group, the relation between OPG and the biomarker mentioned above was not found. Conclusion: OPG may be involved in insulin resistance and inflammation during the pregnancy in women with NGT, but the aforementioned effects of OPG were not obvious in the group of GDM, which may be caused by the reason that other stronger factors in the pathogenesis of GDM have masked the role of OPG.
    cPKCgamma is involved in the regulation of hypoxic preconditioning on CRMP2 hydrolysis and phosphorylation in ischemic cortex of mice
    2012, 32(1):  25-30. 
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    Abstract: Objective To explore the role of cPKCgamma in hypoxic preconditioning (HPC) regulating hydrolysis and phosphorylation of collapsin response mediated protein 2 (CRMP2) in the ischemic cortex of mice. Methods Using our established HPC and middle cerebral artery occlusion (MCAO) BALB/c mouse models (male,18-22g), We applied Western blot, immunoprecipitation (IP) and immunohistochemisty to determine the effect of cPKCgamma activation on CRMP2 hydrolysis and phosphorylation, cPKCgamma-CRMP2 interaction and p-CRMP2 positive cells’ number in the peri-infarct region of MCAO mice. Results We found that HPC could inhibit both the decrease of p-CRMP2 level and increase of BDP in peri-infarct region of ischemic cortex. Pretreatment of cPKCgmma inhibitor Go6983 (6nM) could depress the HPC-induced inhibitory effect on CRMP2 dephosphorylation and hydrolysis in peri-infarct area of ischemic cortex; IP results showed that the activity of cPKCgamma could affect the interaction between cPKCgamma and CRMP2; in addition, the immunohistochemistry results also demonstrated the same effect of cPKCgamma on p-CRMP2 positive cells in peri-infarct region of ischemic cortex. Conclusion cPKCgamma was involved in the regulation of HPC on CRMP2 phosphorylation and hydrolysis in ischemic cortex of MCAO mice.
    Construction of recombinant adenovirus Ad-hBNP and observing the distribution and the expression of hBNP gene in CHF rats
    2012, 32(1):  31-35. 
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    Objective To construct Ad-hBNP and observe the distribution and the expression of hBNP gene in CHF rats. The study could provide a reference of basic research for BNP in gene therapy. Methords The target gene hBNP were obstained by reverse transcription polymerase chain reaction from human heart. The pAd-hBNP were constructed through AdEasy system. Recombinant plasmid pAd-hBNP were packaged and amplified in 293T cells. Ad-hBNP used in gene therapy were acquired. 24 CHF rats were randomly divided into 4 groups for detecting the serum levels of hBNP with ELISA, the expression of hBNP with RT-PCR and immunohistochemistry in different groups. Results Ad-hBNP were constructed successfully and the titer of purified Ad-hBNP was 4.4275×1012VP/ml. Recombinant adenoviruses were showed as polyhedron shape under electron microscopy. The expression of hBNP gene were detected in CHF rats by intraperitoneal injection Ad-hBNP and it continued at least 7 days. Conclusions Ad-hBNP were constructed successfully by using AdEasy system. Exogenous hBNP were effectively expressed in CHF rats. Compared with the half life of BNP, the prosses that at least continued 7 days were significantly extended.
    Pyrrolidine dithiocarbamate attenuates pancreatic β-cell oxidative injury in type 2 diabetic rats
    2012, 32(1):  36-41. 
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    ABSTRACT: Objective To investigate the effect and mechanism of pyrrolidine dithiocarbamate (PDTC) on islet β cell oxidative injury in type 2 diabetic rats. Methods Type 2 diabetic rats model were set up by long-term feeding high-fat foods accompanied with a single intraperitoneal injection of low dose of streptozotocin (STZ, 27mg/kg body weight). The rats in PDTC group were administerd with PDTC (50mg/kg/d) by intraperitoneal injection. After treatment for 1 week, blood glucose levels were estimated. Malondialdehyde (MDA), Superoxide dismutase (SOD) and Glutathione peroxidase (GSH-PX) content in pancreatic tissue were measured. The expression of inducible nitric oxide synthase (iNOS) and the prouduction of nitrotyrosine (NT) in islet tissue were examined by using immunohistochemistry and Western blot. The rate of pancreatic islet β cell apoptosis was detected by flow cytometry. Results The blood glucose level, MDA content, pancreatic β-cell apoptosis, iNOS expression and NT production were significantly higher, while the SOD and GSH-PX activities were significantly lower in DM group than those in normal control group (P<0.01). After administration of PDTC, The level of blood glucose, MDA content, pancreatic β-cell apoptosis, iNOS expression and NT production were significantly decreased, while the SOD and GSH-PX activities were markedly increased when compared with DM group (P<0.01). Conclusion PDTC can reduce the level of blood glucose, relieve the oxidative stress reaction of diabetic rats, suppress the expression of iNOS and NT production, and decrease pancreatic islet β cell apoptosis in diabetic rats.
    Microarray analysis of microRNA expression profile and its roles in reversing drug resistance in gastric cancer cell line SGC7901/DDP
    2012, 32(1):  42-48. 
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    Objective: To analysis the microRNA expression profile and drug resistance characteristic and explore the roles of microRNA-200c on drug resistance in gastric cancer cell line SGC7901/DDP. Methods: Drug sensitivity of SGC7901/DDP and SGC7901 cells were tested by means of MTT assay. microRNA array was used to analyze microRNA expression profile, and bioinformatics analysis was also used to predict possible targets and biological functions of differentially expressed microRNAs. Real-time fluorescent quantitative RT-PCR was used to validate the result of microRNA-200c expression change from microRNA array analysis. And the effects of microRNA-200c on drug resistance were also analyzed in SGC7901/DDP cells by means of cell transfection. Results: The IC50 of cisplatin, doxorubicin, 5-fluorouracil and paclitaxel were significantly higher in SGC7901/DDP cells than in SGC7901 cells (P<0.05). Compared with SGC7901 cells, 5 microRNAs were upregulated more than 2-fold, and 14 microRNAs were downregulated more than 2-fold in SGC7901/DDP cells. The predicted targets both in downregulated and upregulated microRNA expression group were involved in the biological processes of signal transduction, cell cycle, cell differentiation, apoptosis, proliferation and etc. microRNA-200c was confirmed down expressed in SGC7901/DDP cells by real-time fluorescent quantitative RT-PCR. And SGC7901/DDP cells transfected with microRNA-200c precursor displayed significantly decreased IC50 of cisplatin, doxorubicin, 5-fluorouracil and paclitaxel (P<0.05).Conclusion: Changes of microRNA expression profile may be related to the multidrug-resistant in SGC7901/DDP cells, and its reversed drug resistance phenotype may be correlate with the microRNA-200c expression.
    The introduction of error-prone PCR and DNA-shuffling technology to build single-chain antibody library
    2012, 32(1):  56-60. 
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    Abstract:Objective The high-storage capacity single-chain antibody (ScFv) library was constructed for selecting high-affinity antibodies using error-prone PCR and DNA-shuffling. Methods Collected 5ml blood from different age groups, gender and healthy people, mononuclear cells RNA were extracted, reverse transcription. VH and VL genes were amplified by PCR, and then VH and VL gene mutation amplified error-prone PCR. The full-length ScFv fragments were recombinated with the DNA-shuffling technology in vitro, and then transfer E.coli DH5α. The recombination rate was identified by colony PCR, antibody library diversity identified by restriction enzyme digestion. Results The single-chain antibody library was constructed by error-prone PCR and DNA-shuffling techniques. Analyzed by restriction enzyme digestion, the diversity of antibody library was good, the capacity was high storage. Conclusion The introduction of error-prone PCR and DNA-shuffling technology can help improve the genetic diversity, which will help build high-storage capacity of the single chain antibody library.
    The effects of NF-κB in treating hepatopulmonary syndrome in rats with tumor necrosis factor-α monoclonal antibody
    2012, 32(1):  61-65. 
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    AIM: To study the effects of tumor necrosis factor-α monoclonal antibody on the expression of NF-κB on hepatopulmonary syndrome in rats. and the effects of NF-κB in treating hepatopulmonary syndrome in rats with tumor necrosis factor-α monoclonal antibody. METHODS: The experimental models of HPS in rats were built with commom bile duct ligation, and then were injected with TNF-αMcAb (0.1mg/kg.2d)intraperitoneally, Lung histopathological changes were evaluated by hematoxylin and eosin staining; TNF-α concentration in plasma was measured by radioimmunity method ; The endotoxin in the plasma was checked by tachypleus amebocyte lysate kit; Hepatopulmonary syndrome was assessed by measurements of alveoloarterial oxygen difference (P(A-a)O2) . Western blot was employed to investigate the expression and the changes of NF-κB in hepatopulmonary rat lungs. RESULTS: Compared with the CBDL group, there was a gentle vasodilatation and widen interalveolar septum in lung in TNF-α McAb treament group, the level of the P(A-a)O2 was significantly lower (p<0.05). The concentration of ETX and TNF-α were significantly lower(p<0.05). The expression of NF-κB in CBDL+TNF-α McAb group decreased obviously than those in CBDL group(p<0.05). CONCLUSIONS: Tumor necrosis factor-α antibody can attenuate the severity of hepatopulmonary syndrome in rats and reduce activation of NF-κB, Tumor necrosis factor-α antibody can attenuate the severity of hepatopulmonary syndrome through downregulateing the expressiion of NF-κB.
    Mechanism of TGFβ1 and CTGF signaling pathway in experimental hepatic fibrosis
    2012, 32(1):  66-70. 
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    Abstract Objective To investigate the mechanism of TGFβ1 and CTGF signaling pathway in experimental hepatic fibrosis mice. Methods 30 C57BL6/J mice were divided into control group and model group (each group, n=15). Mice were injected with CCL4 which induced liver fibrosis. Serum ALT and HA were tested by enzymic method and radio-immunity. Liver inflammation was graded by HE staining, and liver fibrosis by Masson staining. The expression of proteins of TGFβ1,CTGF,TGFβRII,Smad3,Smad7 of liver tissue were examined by immunohistochemistry. The expression of TGF-β1, Smad3,Smad7 and CTGF mRNA were analyzed by RT-PCR. Results Compared with control group, the expression of TGFβ1, CTGF,TGFβRII, Smad3 proteins and TGFβ1, CTGF,Smad3 mRNA were increased but Smad7 protein and Smad7 mRNA were decreased in model group. The levels of ALT and HA was higher in model group than control group. Conclusion TGFβ1 and CTGF pathway overactivitied may be associated with liver fibrosis. Down-regulating TGFβ, CTGF pathway and up-regulating smad7 expression may be suppressing the hepatic fibrosis.
    Isolation and charcaterization of mesenchymal stem cells derived from whole human umbilical cord applying a direct explant technique
    2012, 32(1):  71-76. 
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    This study was purposed to investigate a techique to isolate mesenchymal stem cells from whole umbilical cord and their biological characteristics. Mesenchymal stem cells(MSCs) were isolated from umbilical cord(UC) by applying a explant technique and explanding cultured in vitro. Growth curves were drawn, and The phenotypes and cell cycle were evaluated by flow cytometry. UC-MSCs was induced to differentiate into adipocytes, osteocytes and chondrocytes in special differentiation condition. Mutidifferentiation related genes and stem cell-related transcription factors, Nanog, Oct-4, Sox-2 were detected by TR-PCR. The result indicated that UC-MSCs were easily isolated using a explant technique, and characteristic of plastic adherence and fibroblast-like morphology. the adherent cells displayed an abundant presence of CD73, CD90, CD105 and absence of CD34 , CD45 , HLA-DR. Cell cycle showed t hat there was percentage of stem cells as G0 / G81.56 % and S + G2 + M18.44% respectively. When cultured in differentiation media, they can be differentiated into adipocytes, osteocytes and chondrocytes. RT-PCR reactions confirmed that their mutidifferentiation related genes were positive.Moreover, stem cell-related transcription factors, Nanog, Oct-4, Sox-2 were positively expressed in UC-MSCs. Based on these findings, the explant technique method is a better method to obtain MSCs from whole human umbilical cord, and UC can be considered as a novel and convenient source of adult MSCs displaying high expansion potential and primitive pluripotent sten cells.
    Establishment of esophageal caner drug resistant xenograft nude mice model
    2012, 32(1):  77-82. 
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    [Abstract] Objective To establish the nude mice model of esophageal cancer drug resistant xenograft and explore the mechanism of esophageal cancer drug resistance. Methods 36 nude mice (BALB/c nu/nu, 4 weeks old) were divided into six groups randomly, 6 each group. Nude mice were respectively inoculated with Eca109 or Eca109/ABCG2 cells subcutaneously in the left subscapularis. xenograft nude mice model was established. The experimental groups received ADM (1, 4mg/kg/3d, respectively), for 7 times. The negative control group received saline. ABCG2 mRNA expression level of xenograft was detected by RT-PCR. ABCG2 protein expression level, cell apoptosis and ADM content in cells of xenograft were detected by flow cytometry. Results The model of esophageal cancer xenograft in nude mice was successfully established, the tumorigenic rate was 100%. After the end of experiment, In the same ADM concentration group, volume, weight and ABCG2 expression of transplanted tumor inoculated with Eca109/ABCG2 cells was significantly higher than transplanted tumor inoculated with Eca109 cells (P<0.05), but the cell apoptosis and ADM content was significantly lower(P<0.05). Conclusion The nude mice xenograft inoculated with Eca109/ABCG2 cells model was an ideal animal model of esophageal cancer drug resistance with ABCG2 resistance phenotype, which provided the ideal animal model for researching the relationship between the ABCG2 and drug resistance of esophageal cancer.
    Serum-free Primary Culture and Identification of Hippocampal Neurons from Newborn Rats
    2012, 32(1):  83-86. 
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    Abstract: Objective To establish a simple and practical method of the serum-free primary culture of hippocampal neurons in vitro to obtain highly purified and energetic neurons. Methods Hippocampus of newborn rats(24 hours) were taken out and digested. Hippocampal neurons were planted in the glass slides covered with Matrigel basement membrane. Twenty-four hours after the cell being plated, the culture medium was removed and replaced by serum-free neurobasal one with N2 and B27 supplementations. The morphological changes of the neurons were observed under inverted phase-contrast microscope at different time. Immunofluorescence staining for β-tublinⅢ was performed to identify the purity of neurons. Results A large number of hippocampal neurons began to adhere to the glass slides and develop small neurites in 3-24 hours. Then, a typical neuronalmorphology appeared on the third day. Up to the 5th day, many neurites extended to form dense network. Soma of neurons became were well developed on the 7th day. Fluorescence staining with β-tublinⅢ showed that the purity of neurons was 94.2±3.6%. Conclusion The hippocampal neurons with high purity can be obtained by the simple and efficient method.
    Expression and significance of MTDH in the breast cancer
    2012, 32(1):  87-88. 
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    Objective Detect the expression of metadherin(MTDH)mRNA and protein and investigate the role of MTDH in the development of breast cancer. Metheds Detect the expression of MTDH protein and mRNA in breast cancer and normal breast tissue by Western bloting and RT-PCR. Analyze the relatives of MTDH protein and clinicothological parameters. Results The positive rate of MTDH protein were significantly higher in cancer tissues than that in normal breast tissues(P<0.05). The expression of MTDH in cancer tissue were correlated with lymphnode metastasis(P<0.05) and were not correlated with TNM stage and differentiation grade. The positive rate of MTDH mRNA were significantly higher in cancer tissues than that in normal breast tissues(P<0.05); The expression of MTDH mRNA were significantly higher in cancer tissues than that in normal breast tissues(P<0.05). Conclusion The carcinogenesis , invasion and metastasis of breast cancer may be related to overexpression of MTDH protein and mRNA.
    Ang II promotion of VSMCs proliferation and migration in rats
    2012, 32(1):  89-91. 
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    Sodium metasilicate inhibited reactive oxygen species production by rabbit peripheral leukocytes
    2012, 32(1):  94-95. 
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    Abstract Objective Recent studies have suggested that Na2SiO3 treatment suppresses the development of atherosclerosis in hyperlipemia animal models, to obtain further insight into the mechanism of antiatherothrombotic action of Na2SiO3, we studied the effect of Na2SiO3 on the production of reactive oxygen species( ROS)by peripheral blood leukocytes(PBL) . Methods 1. PBL separated from health male New Zealand White(NZW)rabbits were exposed to ox-LDL and Na2SiO3 , and then ROS in the cells was quantified by the addition of 2',7'-dichlorofluorescein diacetate (DCFH-DA) . 2. Male NZW rabbits were fed a 0.5% cholesterol diet for 4 weeks. At 4 weeks , the animals were randomized to Na2SiO3 in the drinking water(Na2SiO3 group) or water alone(hyperlipemia group), PBL were separated from the animals to compare the production of ROS . Results Na2SiO3(8.5 mg/L of silicon) treatment dramatically decreased ROS production by ox-LDL stimulated PBL in vitro(fluorescent intensity: 53.3±0.9 vs 61.0±3.1,p﹤0.01). Although there was no significant difference in blood cholesterol levels between the two groups, ROS levels were significantly lower in PBL of Na2SiO3 group than in hyperlipemia group: fluorescent intensity with stimulation of PMA was 36.55±4.59 and 41.20±3.04(p﹤0.05)respectively,without PMA was 33.15±0.49 and 37.43±0.59( p﹤0.01). Conclusions sodium metasilicate inhibited ROS production by ox-LDL-stimulated peripheral leukocytes of rabbit.Administration of Na2SiO3 as drinking water to hypercholesteremia rabbits inhibited the priming and activation of the PBL,and this effect was unrelated to blood cholesterol lowering.
    Progress of hypoxia and heart rate variability research
    2012, 32(1):  99-101. 
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    Abstract: Hypoxia has been implicated in the pathogenesis of cardiovascular diseases, respiratory diseases and tumors that results in heart rate variability (HRV) changes. The change of HRV is related to a lot of diseases, so HRV is often used in clinic as an important index to predict or evaluate diseases. The newly clinical application of HRV and research progress of hypoxia on HRV are reviewed briefly.
    Edema, fever, paraplegia
    2012, 32(1):  106-112. 
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