Table of Content

25 May 2009, Volume 29 Issue 5

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研究论文

Association of Ala22/72Ser polymorphism in the catechol-O-methyltransferase gene with schizophrenia and preliminary functional exploration

Yan WANG; Yue FANG; Qi XU

2009, 29(5):  449-452. 

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Objective Study the association of the Catechol-O-methyltransferase (COMT) gene single nucleotide polymorphism (SNP) Ala22/72Ser with schizophrenia (SCZ) in northern Han Chinese population. Methods In 506 SCZ patients, the genotype of Ala22/72Ser of COMT gene was determined by polymerase chain reaction (PCR) - sequencing; the severity of psychiatric symptoms of SCZ patients was scaled by positive and negative syndrome scale (PANSS); the expression of COMT gene was determined by Real-Time PCR. Results In SCZ patients, the overall score of positive scale and general psychopathology scale was significantly increased in Ser and Ser/Ala carriers (P < 0.01), and the expression of COMT gene in these patients was significantly decreased (P < 0.01). Conclusions The polymorphism of Ala22/72Ser in COMT gene was associated with the severity of positive symptoms and general psychopathology symptoms in SCZ patients, and associated with the expression of COMT gene.

NSPc1 is a Necessary Factor for HeLa Cells' Proliferation

Guang-yu HU; Xu-dong WU; Xiao-zhong PENG; Yan-hua GONG

2009, 29(5):  453-458. 

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Objective To study the effect of PcG member NSPc1 on the proliferation of HeLa cells. Methods Using bioinfomatic analysis to design the siRNA sequence for knockdown NSPc1. Detecting the expression level of NSPc1 in HeLa cell lines using semi-quantitative RT-PCR, Real-time PCR and Western blot after transfection of the designed siRNA. Transient transfecting pSUPER-NSPc1 into Hela cells and performing BrdU incorporation assay. Establishing NSPc1 stably knockdown cell lines, comparing proliferation abilities with the control cells. Results 1) The designed siRNA could efficiently knockdown the expression of NSPc1; 2) Transient knockdown NSPc1 could repress BrdU incorporation; 3) The established NSPc1-knockdown cell lines had a significantly low proliferation rate than control cells. Conclusion The expression of NSPc1 is necessary for the normal proliferation of HeLa cells.The NSPc1 stably knockdown cell pools is a useful model for further study the pathway related to NSPc1.

The cloning and expression of Par6A cDNA

Xiao-jun LIU; Xing-xing KONG; Liu-lian ZHU; An-fang CUI; Shao-wei JI; Yong-sheng CHANG; Fu-de FANG

2009, 29(5):  459-463. 

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Objective　 The cloning and expression of Par6A. Methods Par6A cDNA was amplified from rat L6 skeletal muscle cells by RT-PCR and the cloning and expression vectors of Par6A were constructed. The expression vector were transfected into 293 cells. Furthermore, the function of Par6A was confirmed by Co-immunoprecipitation. Results Par6A cDNA with approximately 1kb in length was successfully amplified, and the expression vector of pDsRed-Express-N1-Par6A was constructed. The red fluorescene was seen under fluorescent microscope after 293ET cells were transfected for 24h using the pDsRed-Express-N1-Par6A vector. The expressed Par6A protein can interacted with PKCζ.　 Conclusion　 We successfully cloned the Par6A cDNA from rat L6 skeletal muscle cells, which provided a reliable material to study the function of Par6A.

SCN1A promoter mutations screening of Dravet sydrome

Yue-sheng LONG; Shao-peng LIN; Yi-wu SHI; Wei-ping LIAO

2009, 29(5):  464-467. 

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Objective To screen and analyze the mutations of SCN1A promoter regions from the patients with Dravet syndrome and to evaluate the relationship between mutation and disease. Methods Peripheral blood of 24 patients with Dravet syndrome and 100 unrelated normal persons were collected and genomic DNA was extracted. PCR and sequencing of SCN1A promoter region were then performed. To evaluate the possibility of mutation inducing disease, bioinformatics analysis was applied to analyze the conservation of the sequences including the mutation sites and predict the potential transcriptional elements. Results Two mutations -244 C>T and -662 G>- were identified from patients and both mutations were found in their farther genomic DNA, but not in unrelated normal persons. The nucleotide sites -244 and -662 were highly conserved in mammals (100%). The average nucleotide identity rate of -244 adjacent sequences (20 bp upstream and downstream of the mutation sites) between human and other species was 90%, and was only 60% for that of -662. Potential transcription regulatory elements and factors were found on the sequence including -244, but not on the sequence including -662. Conclusions The two mutations may be associated with Dravet syndrome and further studied should be performed to demonstrate their pathogenic mechanisms.

Construction，transfection and activity identification of eukaryotic expression vector of human PRKCB1 containing enhanced green fluorescence protein gene

Lian DUAN; Bo ZHOU; Qi-fu LI

2009, 29(5):  468-474. 

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Objective To construct the eukaryotic expression vector of human PRKCB1 containing enhanced green fluorescence protein gene and transfer into human umbilical vein endothelial cell(HUVEC)，then identify activity of expression protein. Methods The pReceiver-M29-PRKCB1 eukaryotic expression plasmid was constructed by frame amplified from pMD18-T-PRKCB1 plasmid. Then the recombinant plasmids were identified by enzyme analysis and DNA sequencing. According to optimized conditions,the eukaryotic expression plasmids were transfered into HUVEC and observed under fluorescence microscope.After that, transfection efficiency was calculated under random vision.The plasma membrane/cytosol ratio of fluorescence was calculated under confocal microscopy.The translocation was identified. Results The gene sequence was completely consistent with that reported in GenBank. The enhanced green fluorescence protein could be observed in HUVEC after 48 hours.Transfection efficiency was 18.62±0.57%. The translocation was observed. Conclusion The eukaryotic expression plasmid is successfully constructed and transfered into HUVEC, the translocation was identified. It is the molecular instrument for screening HUVEC stably expressing human protein kinase c 2 and isolating protein complex.

Hepatitis B virus X protein effect on nuclear localization and transcription of β-catenin in human liver cell

Hao DING; Liu-xing HE; Tao FENG

2009, 29(5):  475-478. 

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Objective To investigate the interaction between HBx protein and β-catenin, illuminate the molecular mechanism in HBV-associated hepatocellular carcinoma Methods Iimmunocytochemistry and Luciferase Assay methods were used to detect the endocellular location and transcriptional activity of β-catenin in normal liver cell line L02 infected by Ad-HBx. Results Iimmunocytochemistry: there was markedly increased expression of β-catenin in cytoplasm and nucleus. Luciferase Assay: Ad-HBx led to significantly increase of TCF-4-dependent transcription, the TOP count of Ad-GFP and Ad-HBx-GFP was 1.4 fold and 11fold compared to the negative control respectively(P<0.05). Conclusions There is the expression of HBx protein in human normal liver cell line (L02) with infected Ad-HBx. This data suggests that ectopic expression of HBx results in the accumulation ofβ-catenin in the cytoplasm and subsequent nuclear localization and increases TCF-4-dependent transcription in L02 cells. HBx protein is essential for the activation of wnt/β-catenin signaling by increasing the expression of β-catenin in normal liver cells.

Adipose-derived MSCs inhibit the proliferation of chronic myeloid leukemia cells K562 through secreting DKK-1

Ya-shu ZHU; Zhao SUN; Zhen-ya LI; Qin HAN; Jing LI; Chun-hua ZHAO

2009, 29(5):  479-483. 

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Objective To investigate the molecular mechanism of mesenchymal stem cells' inhibitory effect on the proliferation of chronic myeloid leukemia cells K562. The purpose of this research was to provide the basic and clinical rational for leukemia treatment. Methods Adipose-derived mesenchymal stem cells (MSCs) were co-cultured with K562 cells. Then, the proliferation rate of K562 and the gene expression of WNT signaling pathway in K562 were detected using neutralizing antibodies assays, 3H-TdR assays, cell-cycle assays, RNA interference and real-time PCR assays. Results After co-cultured with MSCs, the proliferation rate of K562 was decreased by 77%（P<0.05）, the proportion of K562 cells in G0/G1 phase was significantly higher (62.1%±5.8%) than that (45.2%±6.9%) in K562 cells cultured alone（P<0.05）. And MSC's inhibitory effect was not influenced by the transwell system that prevents the direct interaction of MSCs and K562 cells. We performed neutralizing antibodies assays and found that it was DKK-1 that participated this anti-proliferative process. When the expression of DKK-1 was down-regulated by RNA interference, MSCs' inhibitory effects on K562 cells proliferation were attenuated, and the activity of WNT signaling pathway in K562 cells was partially recovered through detecting the accumulation of β-CATENIN and the expression of c－MYC, CYCLIN D2. Conclusions Adipose-derived MSCs could inhibit the leukemia K562 cells' proliferation by secreting DKK-1, which mediated the suppression effects on WNT signaling pathway of K562 cells and resulted in arresting the cell cycle at G0/G1 phase.

Distribution of unnormal circulation vascular resistance and analysis of cardiac function in Guangxi

Jing-wen CAO; Bao-shen QI; Xiao-mei ZHOU; Shao-mei HAN; Hui LI; Guang-jin ZHU

2009, 29(5):  484-487. 

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Objective To analyze the distribution features of systemic vascular resistance(SVR)disorder in healthy subjects in Guangxi province．Methods SVR and systolic blood pressure(SBP)，diastolic blood pressure(DBP)，mean arterial pressure(MAP)，pulse pressure(PP)， cardiac output(CO)，cardiac index(CI)，stroke volume(SV)，stroke index(SI)，left ventricular ejection time (LVET)，left cardiac work (LCW) and cardiovascular function were determined with Bioz.com Cardio Dynamics． Results The incidence of SVR disorder in youngster and elder was higher than other subjects .The prevalence of SVR disorders was higher among females than among males(P <0.001)．SVR was positively correlated with SBP，DBP，MAP，PP，LVET，body mass index(BMI)，HR was inversely associated with CO，CI，SV，SI，LCW，and arterial compliance(AC)．CO and MAP were independent influencing factors with SVR．Conclusion SVR disorder is associated with age，sex，and blood pressure in populations in Guangxi province and may be a marker of vascular injury．

Polymorphism of 17Y-STR loci in three minority populations in Guangxi of China

Zuo-ren LIANG; Chang-hui LIU; Chao LIU

2009, 29(5):  488-494. 

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Objective 　To investigate the polymorphism of seventeen Y chromosomal short tandem repeat (STR) loci in dong, miao and zhuang nationality of Guangxi of China，and then evaluate their forensic application．Method the DNA was extracted from oral swab of minority population with magnetic beads method and then seventeen Y-STR loci were amplified with AmpFlSTR? Yfiler? PCR Amplification Kit System . The PCR products were detected with 3l30XL Genetic Analyzer. The allele frequencies，diversities and genetic distance were calculated ．Results The information of allele distribution and gene diversity of the 17 Y-STR loci were obtained from three minority population.275 haplotypes were found in three minority population. The haplotype diversity of dong nationality is 0.9972,Miao is 0.9966,zhuang is 0.9964.The genetic distance between dong and miao nationality is most close. Conclusion The haplotype diversity of 17 Y-STR loci is so effective in racial identification, paternity identification and human population genetics.

Construction of pGL3-Basic-SREBP-1c-promoter reporter gene vector and detection of its function

Xiao-jun LIU; Xing-xing KONG; Rui WANG; Di SHAO; Ai-jun QIAO; Yong-sheng CHANG; Fu-de FANG

2009, 29(5):  495-498. 

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Objective To construct human SREBP-1c-promoter reporter gene vector and to detect its function.　 Methods Human blood genome DNA was extracted and pGL3-Basic-SREBP-1c-promoter reporter gene vector was constructed. Furthermore, the function of SREBP-1c-promoter was confirmed by dual-luciferase reporter assay. Results　pGL3-Basic-SREBP-1c-promoter reporter gene vector was constructed successfully and the promoter activity was obviously repressed by co-transfection FoxO1. Overexpression FoxO1 inhibited the SREBP-1c protein expression. Conclusion　FoxO1 repressed the SREBP-1c protein expression by inhibition the SREBP-1c transcription.

Expression and purification of nine nucleocapsid proteins from coronaviruses

Hui-wen XU; Yan-bin WANG; Chao WU; Jian-wei WANG; Tao HONG

2009, 29(5):  499-503. 

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Objective To explore the conditions of the expression and purification of nucleocapsid proteins of human coronaviruses SARS-CoV, HCoV-229E, HCoV-OC43, HCoV-NL63 and HCoV-HKU1, animal coronaviruses bovine coronavirus, murine hepatitis virus, feline infectious peritonitis virus, and infectious bronchitis virus. Methods The pET-30a-based recombinant plasmids containing full length nucleocapsid(N) protein of coronaviruses were transformed into E.coil BL21(DE3) competent cells and were induced to express N proteins by IPTG. The expression products were purified by ion exchange chromatography and Ni 2+ affinity chromatography, and were verified by SDS-PAGE and Western blot. Results Nine coronavirus nucleocapsid proteins with correct Mr were solubly expressed and highly purified with purity >90%. Conclusion Successfully expressed nine recombinant nucleocapsid proteins with high purity in E. Coli，which provides materials to study the function of these coronavirus N proteins.

The role of fibroblast growth factor 23, matrix extracellular phosphoglycoprotein and secreted frizzled related protein 4 in pathogenesis of tumor induced osteomalacia

Yan JIANG; Wei-bo XIA; Xun-wu MENG; Xiao-ping XING; Mei LI; Ou WANG; Ying-ying HU; Huai-cheng LIU; Xue-ying ZHOU

2009, 29(5):  504-509. 

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Objective To evaluate the role of Fibroblast growth factor 23 (FGF-23), matrix extracellular phosphoglycoprotein (MEPE) and secreted Frizzled related protein 4 (sFRP4) in pathogenesis of Tumor induced osteomalacia (TIO). Methods The mRNA expressions of FGF23, MEPE and sFRP4 were detected in 8 TIO tumors (T1-T8), 5 other mesenchymal tumors(C1-C5), 2 normal bone tissues(B1-B2), 2 normal muscle tissues(M1-M2), blood clotting and necrostic tissue of bone in a patient of hypophospatemic osteomalacia but not TIO (P1) using RT-PCR. The FGF23 protein expressions were analyzed in 8 TIO tumors using Western blot. Serum FGF23 (ELISA) and phosphate were measured before and after operation in 4 TIO (T1、3、4、7) and P1 patients. Results The mRNA of FGF23 and MEPE were expressed in TIO tumors abundantly. Some TIO tumors expressed sRP4 mRNA. 7/8 TIO tumors expressed various FGF23 protein in Western blot. Serum FGF23 in TIO patients were elevated, and went down after tumor resections in 2～24 hrs. The increase of serum phosphate was slower than FGF23 degression (in 3～10 days). Serum FGF23 was stably high in P1 patient without serum phosphate elevation. The expressions of FGF23 and MEPE mRNA were related (r＝0.884，P＜0.05). There was a positive relationship between FGF23 mRNA and protein expression (r＝0.921，P＜0.05). Conclusions FGF23 plays an important role in pathogenesis of TIO. MEPE may be involved in pathogenesis of TIO. The role of sFRP4 in pathogenesis of TIO is still need to be explored.

Effects of high-density lipoprotein and lipoprotein-deficient serum on intracellular cholesterol efflux in patients with type 2 diabetes mellitus

Hui-juan WANG; Xiao-mei MENG; Lian-feng CHEN; Quan FANG; Xiao-wei YAN

2009, 29(5):  510-514. 

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Objective In the present study, we examined the effects of high-density lipoprotein (HDL) and lipoprotein-deficient serum (LPDS) isolated from patients with type 2 diabetes mellitus on cholesterol efflux through human skin fibroblast (HSF) and human hepatoma cell line (HepG2). Methods and Results Blood was collected from 13 patients with type 2 diabetes mellitus and 17 healthy volunteers and HDL and LPDS were isolated. Cholesterol efflux assays, RT-PCR and western blot were performed on HSF and HepG2 cells. The HepG2 cells have a high expression of scavenger receptor B1(SR-B1) and lack functional ATP-binding cassette receptor A1(ABCA1) and ATP-binding cassette receptor G1(ABCG1) while HSF cells express SR-B1 at very low levels and have a high expression of ABCA1 pretreated with 22-OH cholesterol. The cholesterol efflux from HepG2 cells to HDL isolated from patients with diabetes decreased significantly compared to controls. However, cholesterol efflux from HSF cells to LPDS was not different between groups. Conclusion The function of HDL involving cholesterol efflux in type 2 diabetes mellitus was impaired while cholesterol efflux induced by LPDS from HSF cells was maintained, implying that HDL plays a critical role in mediation of intracellular cholesterol accumulation and progression of atherosclerosis in patients with type 2 diabetes.

VLDL promotes the expression of lipoprotein lipase in human glomerular mesangial cells

Jing LI; Hang;LI; Yu-bing WEN; Xue-wang LI

2009, 29(5):  515-518. 

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Objective To study the expression of lipoprotein lipase (LPL) in human glomerular mesangial cells and effect of very low-density lipoprotein (VLDL) on the expression of LPL . Methods In human glomerular mesangial cells, LPL mRNA expression, protein synthesis and activity were detected by RT-PCR, western blot and a chemical analysis bases on the radioisotope, respectively. Effect of VLDL on the expression of LPL in mesangial cells was detected by western blot. Results In human glomerular mesangial cells, a single 276-bp band, specific for human LPL, could be seen by RT-PCR, and a single 55kd band, specific for human LPL, could be seen by western blot. LPL activity of mesangial cells was also detected in the medium after release by heparin. VLDL stimulated LPL protein synthesis in mesangial cells in a time- and dose-dependent manner. Conclusion LPL is expressed by human mesangial cells and it has catalytic activity. Expression of LPL in mesangial cells is regulated by VLDL.

Relationship of plasma homocysteine levels and pathological changes of large artery in stroke patients

Bin PENG; Shan;GAO; Bing-qi YU; Ying-hui ZHU; Bo WANG

2009, 29(5):  519-521. 

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Objective To determine the association between homocysteine and severity of large cerebral artery atherosclerosis .Methods 152 ischemic stroke patients were evaluated using transcranial Doppler (TCD)，magnetic resonance angiography ( MRA) or digital substraction angiography (DSA) Severity of cerebral artery atherosclerosis was scaled by number of stenosed or occluded artery . Results-There were 83 patients in low level homocysteine group with mean Hcy level 15.31±3.08?mol/L, while 69 patients in high level homocysteine group with mean Hcy 34.47±21.38?mol/L . Number of diseased intra- and extra- artery was 1.86±1.51 and 1.52±1.46 respectively. No significant difference was demonstrated in the number of diseased artery between two groups. No correlation was noted between Hcy and number of diseased artery.Conclusions-There was no association between high homocysteine and severity of large cerebral artery atherosclerosis in ischemic stroke patients. Homocysteine might be the marker of the disease.

Expression reinforcement of Hedgehog Signaling Pathway in Patients with Breast Cancer

Hai-lin HUANG; Zhi-qing HU; Wei-min WANG; Yi WANG; Qing-ping CAI; Qiang WANG

2009, 29(5):  522-526. 

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Objective To investigate whether the Hedgehog (Hh) signaling pathway is activated in human breast cancer. Method we determined the expression of the components, including Sonic Hh, Patched1, and Gli1,Smo of the Hh pathway by immunohistochemical staining in a series of 64 human breast carcinomas. Result In the 64 tumor specimens,there are 64（100％），61（95.3％），62（96.9％）,and 61（95.3％）highly express protein Shh ,Patched1 and Glil,Smo respectively,while in the adjacent nomal mammary tissue, correspondingly,those protein are not or weakly express at can detect level.So,the Hedgehog (Hh) signaling pathway is activated in human breast cancer commonly,which has no relative to lymphoid node metabasis，or size of carcinomor, but maybe connect with some special histologic type. All of 64 tumors displayed staining of high intensity for Gli1 when compared with adjacent normal tissue. The ratio of nuclear positive staining of Gli1/all the tumor cell is range from 2%－95%,median at 40.87%. The nuclear staining of Gli1 correlated with expression of estrogen receptor (P<0.05），but has no relative to progesterone receptor。Conclusion These data indicate that the Hh pathway is a new candidate for therapeutic target of breast cancer.

Effects on Livin antisense oligodeoxynucleotides of the proliferation and apoptosis of HL60 cells

Kang ZHOU; Jian LAN

2009, 29(5):  527-530. 

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Objective To investigate effects of the proliferation and apoptosis of human leukemia ( HL60) cells by Livin antisense oligodeoxynucleotides (ASODN)．Methods to detect expression of Livin protein on HL60 cells by immunohistochemistry. Specific phosphorothioate antisense oligodeoxynucleotides and missense oligodeoxynucleotides target Livin mRNA were synthesized and was transfected into HL60 cells following cationic liposome．The proliferation inhibitory rates of HL60 cells were assessed by MTT method. The expressions of Livin mRNA were detected by RT-PCR. Transmission electron microscope and TUNEL technology were used to detect the apoptosis level and morphologic changes. Results ASODN at a final concentration of 600 nmol／L could inhibit the HL60 cells proliferation and the expressions of Livin mRNA. The apoptosis rates detected by TUNEL was 38.48%±4.37%. cellar ultrastructure was markedly destroyed by Livin ASODN. There were significant differences compared with the control group (P<0.01).Conclusion Livin ASODN could suppress HL60 cell proliferation effectively, decline Livin gene expression, induce significant apoptosis of HL60, therefore Livin gene may become a new target for gene therapy of leukemia.

Zinc suppressed the airway inflammation in asthmatic rats through the regulation of Th1/Th2 balance and activity

Hong-yan LU; Xu-qin MAO; Yu WEN; Ling ZHANG

2009, 29(5):  531-534. 

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Objective To investigate the effects and mechanism of zinc on the airway inflammation in asthmatic rats. Methods Animal models of asthma were established by OVA challenging, zinc-deficient and zinc-supplementary diet were given accordingly. Thirty-two SD rats were divided into four groups according to weight: zinc-deficient diet with OVA challenge group (group A), zinc-supplementary diet with OVA challenge group (group B), zinc-normal diet with OVA challenge group (group C) and zinc-normal diet with saline challenge group (group D). Twenty-four hours after asthma was induced, the right lungs were harvested for HE staining to observe histomorphological changes and the respective numbers of eosinophils, neutrophils and macrophage in the bronchial mucus were counted. The contents of interferon-γ (IFN-γ), interleukin-4 (IL-4), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione pexoxidase (GPX) in lung homogenate were detected. Results Compared with group C, the numbers of eosinophils, neutrophils and macrophage, and the contents of SOD increased in group A, but decreased in group B significantly (P<0.05). At the same time, the contents of IFN-γ, SOD, GPX and the percentage of IFN-γ/IL-4 decreased in group A but increased in group B significantly (P<0.05), no significant difference was observed for IL-4. Conclusion Zinc may suppress airway inflammation in asthmatic rats through the regulation of Th1/Th2 balance and antioxidant.

技术与方法

The new method of inducing mouse primordial germ cells into hepatocytes-like in vitro

Hua-fang ZHAO; Xiao-yan LI; Xiao-lin SHI

2009, 29(5):  535-538. 

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Objective To establish a new method for inducing the primordial germ cells to differentiate into hepatocyte-like in vitro. Methods The primordial germ cells (PGCs) from the gonadal ridges of the mouse embryos at 13 days postcoitum from Kunming pregnant mice were cultured and proliferated in vitro. Then embryonic hepatocytes enclosed in microcapsule and liver tissue extract of newborn mice were added into medium to co-culture with PGCs for committed differentiation. Albumin (ALB) and α-1-antitrypsin (AAT) were assayed by immunocytochemistry. Results The morphology of cells differentiated from PGCs likes star or ovum, the ALB and AAT immune positive expression were detected in those differentiated cells. The ratio of positive cells was above 70% in 2 weeks. Conclusion Microenvironment of embryonic hepatocyte microcapsules and liver tissue extract could effectively induce PGCs to differentiate into hepatocytes.

临床园地

Langerhans cell histiocytosis of the infundibulum in adult: a case report

Ji-wei HUANG; Ding-rong ZHONG; Xiao-ying YAO; Wan-chen DOU

2009, 29(5):  539-541. 

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Objective To investigate the clinical feature, diagnosis and therapy of isolated langerhans cell histiocytosis (histiocytosis X) with involvement of a rare site. Methods A case of isolated langerhans cell histiocytosis of the infundibulum in adult was reported. The endocrinic tests, imaging , immunohistochemical and pathological examinations of this case were detected．Through literature review, the pathological and clinical feature, diagnosis , therapy of isolated langerhans cell histiocytosis of the infundibulum were overviewed. Results Magnetic resonance imaging (MRI) of the brain showed a 9-mm homogeneously enhancing mass in the region of the infundibulum. No other lesions were found in other organ systems. The patient underwent an occupying lesion resection of the Infundibulum via right pterion approach. Langerhans cell histiocytosis was diagnosed through pathologic analysis. She was on hormone replacement therapy and close follow-up visit postoperatively Conclusion Isolated langerhans cell histiocytosis of the infundibulum in adult is extremely rare. Understanding of this disease should be deepened to avoid of misdiagnosing and mistreating.

Subcutaneous Panniculitis-like T-Cell Lymphoma: A Report of 6 Cases and Review of the Literatures

Yong-jun YANG; Jie SHI; Quan-cai CUI

2009, 29(5):  542-546. 

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Objective To investigate the clinical and pathological characteristics as well as immunophenotypes of subcutaneous panniculitis-like T-cell lymphoma to explore its differential diagnosise with other similar diseases. Methods The clinical、histological and immunophenotypic features of 6 cases were described in detail and related literatures were reviewed. Results All of 6 patients presented with subcutaneous nodules or /and erythematous plaques without lymph nodes swelling and with fever but one case , one developed to ulcer from its nodules. All of 6 patient presented typical histological changes and 2 of them associated with prominent hemophagocytic syndrome. The neoplastic cells were of T-cell phenotype. Two patients followed an aggressive clinical course with short survival of 9-16 months and four patients who treated with chemotherapy have an improved survival, but two of them had been recurred. Conclusions　SPTCL is a rare type of T-cell lymphoma which has a clinical and pathological characteristics relatively, and it should be differentiated from benign panniculitis or other lymphomas of the skin.

研究短文

Effect of donor characteristics on the immunologic composition of the mixture of G-CSF mobilized peripheral blood stem cell grafts and G-CSF primed bone marrow grafts

Ming-rui HUO; Ying-jun CHANG; Xiang-yu ZHAO; Xiao-hua LUO; Xiao-jun HUANG

2009, 29(5):  547-548. 

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Atorvastatin on Inflammatory Markers of the Patients Undergoing Precutaneous Coronary Intervention

Jian-bing WANG; Yu-ming HAO; Dong-ying WANG; Rong-hong LIU; Hui SHAO; Yu-ling CUI

2009, 29(5):  549-550. 

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短篇综述

The advance in researches on the proteomics of type 2 diabetes

Yang LIU; Yu-min GUI; Bo YUAN; Yan MENG

2009, 29(5):  551-554. 

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Comparing the patients of the type 2 diabetes with the control and using the animal models, the researchers have found some disease related proteins and the marks for disease detection. They have set some new detection methods and give us a special view into type 2 diabetes. We do some primary discussions about recent studies and future development directions in the proteomics study of type 2 diabetes in this article.