Table of Content

25 June 2009, Volume 29 Issue 6

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研究论文

α-Synuclein phosphorylation takes part in mouse dopaminergic neuron protection

Qi LIU; Chun-li DUAN; Bo WU; Chun-xiang FAN; Tao ZHANG; Huan-ying ZHAO; Chun-li ZHAO; Hui YANG

2009, 29(6):  561-565. 

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Objective To study the role of phosphorylation at Serine 129 in regulating the neurotoxicity of α-synuclein. Methods Wild type and phosphomimic mutant α-synuclein genes were over-expressed in mouse dopaminergic cells MN9D using retrovirus. The cell viability was examined using CCK-8 assay and the cell morphology was observed with immunofluorescence microscope. Results The result of real time PCR showed that WT/α-SYN and S129D/α-SYN genes were overexpressed in MN9D as compared to uninfected MN9D and vector control group. The cell viability was obviously reduced when over-expressing both WT and phosphorylated α-synuclein genes. And the viability of cells over-expressing wild type α-synuclein was much lower than that of the cells over-expressing phosphorylated α-synuclein. Insoluble α-synuclein aggregates were observed with confocal microscope in the MN9D cells over-expressing phosphorylated α-synuclein. Conclusion Over-expressing phosphorylated α-synuclein may lead to α-synuclein aggregated in MN9D cells. Phosphorylated at 129 Ser may protect the cells from toxicity induced by over expressing α-synuclein.

Bone marrow stromal cells of GFP transgenic mice differentiate into neuron-like cells in vitro

Hui ZHOU; Xiao-dan JIANG; Ru-xiang XU; Ying-qian CAI; Yu-xi ZOU

2009, 29(6):  566-569. 

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Objective To induce bone marrow stromal cells (BMSCs) of green fluorescent protein (GFP) transgenic mice (GFP-GM-BMSCs) into neural-like cells in serum-free medium. Methods GFP-GM-BMSCs were isolated and cultured in serum-containing medium. Then the 3rd generation of GFP-GM-BMSCs were transferred into serum-free medium containing 2% B27 supplement with 20ng/ml basic fibroblast growth factor (bFGF) and 20ng/ml epidermal growth factor (EGF).At the 5th day after culture, the expression of Nestin was examined by immunofluorescence. NSE, GFAP were examined by immunocytochemistry on the 10th day following the induction. Results The induced GFP-GM-BMSCs gradually became circular. Some of them grew long processes and showed the neuron-like appearance. The positive expression of Nestin was about (40.24%+5.09)% on the 5th day, while the NSE and GFAP were positively expressed as (36.43+5.27)% and (49.73+6.28)% respectively on the 10th day after the culture. Conclusions GFP-GM-BMSCs could be induced into neuron-like cells in serum-free medium containing EGF and bFGF.

Establishment of a cell model supporting HCV1b subgenomic replication

Hui-yi CHEN; Jie LU; Rui LI; Zhong-shuai XIN; Bin YIN; Yan-hua GONG; Xiao-zhong PENG

2009, 29(6):  570-574. 

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Objective To establish a cell model for supporting HCV 1b subgenomic replicon of Chinese population replication in vitro. Methods Plasmid containing HCV 1b subgenomic replicon of Chinese population was constructed. HCV subgenomic replicon RNA was obtained by in vitro transcription, and then delivered into Huh-7.5.1 cell by electroporation. After screening the cells in the medium containing G418, we detected the HCV RNA in the obtained cell colonies by RT-PCR and the HCV NS5B protein by western blot. The cell colonies which contain HCV RNA were treated by IFN in order to observe the diminished level of HCV RNA. Results In 4-6 weeks after transfection, visible colonies were stained by crystal violet. The HCV subgenomic replicon RNA NS4B was detected by RT-PCR and the HCV NS5B protein was detected by western blot. After treatment by IFN, HCV RNA diminished obviously. Conclusion We have successfully established a cell model for supporting HCV 1b subgenomic replicon of Chinese population replication in vitro. This cell model is a useful tool for the study on HCV pathogenesis, the screening of antiviral drugs and the development of vaccines.

Differentiation of mouse embryonic stem cells into insulin-secreting cells in vitro

Ai-min DENG; Ge LIN; Guang-xiu LU

2009, 29(6):  575-579. 

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Objective To establish a system in which mouse embryonic stem cells (mESCs) differentiated into insulin-secreting cells in vitro. Methods mESCs were cultured to form embryoid bodies(EBs). EBs were cultured in serum-free medium to obtain nestin-positive cells. The selected nestin-positive cells were expanded, then added different concentration of nicotinamide into the medium to induce the differentiation of nestin-positive cells. Immunochemistry staining and flow cytometry analysis were made on 15th day after induction. Results Flow cytometry analysis revealed that the percentage of nestin-positive cells were different from EBs whose diameters were different. The percentage of nestin-positive cells from EBs whose diameters about 100μm were higher than those of other diameters. Nestin-positive cells induced by nicotinamide could form islet-like cell clusters. Flow cytometry analysis revealed the percentages of insulin-positive cells were different after induced for 15 days by different concentration nicotinamide. Percentage of insulin-positive cell induced by 10mmol/L nicotinamide were higher than those induced by 0 mmol/L or 5 mmol/L nicotinamide(P<0.01). The induced cells could secrete insulin in responding to different glucose concentration. Conclusion More insulin-secreting cells could be obtained from the nestin-positive cells after treatment of 10 mmol/L nicotinamide for 15 days.

Gene mutation analysis in a patient with Wiskott-Aldrich syndrome and custom prenatal testing for the family

Yan DIAN; Yan MENG; Feng-xia YAO; Zheng WANG; Yuan-yuan PENG; Xiao-qiao LI; Shang-zhi HUANG

2009, 29(6):  580-583. 

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Objective To determine the mutations of WASP in a WAS patient, and to make custom prenatal testing for the family. Methods Blood samples of the individuals in this family were collected. Mutation screening was carried out by PCR and direct sequencing. 21-trisomy testing and the gender of the fetus were carried out before gene diagnosis for the mutation. Results A double-nucleotide-deletion (c.107-108delTT) was identified in exon 1 of WASP gene in the proband and his mother. The fetus is female and acquires the normal from the mother. Conclusion The c.107-108delTT mutation was responsible for the disease in the family. The fetus is a normal female.

The differentiation of transplantating mesenchymal stem cells （BMSCs）into pancreas in diabetic rat and the effect on blood glucose

Ya-li YANG; Feng GAO; Hui QI; Fu-rong LI

2009, 29(6):  584-588. 

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Object To investigate the cell distrubution and the therapeutic effect after BMSCs were injected into subcapsular pancreas of diabetic rats model. Methods BMSCs of male SD rat were labeled with enhanced green fluorescent protein (EGFP), administered them to diabetic rats by injecting into subcapsular pancreas at multiple points. Blood glucose levels, blood insulin levels and blood C-peptide levels were monitored, distribution of BMSCs were monitored by EGFP detection at 8 weeks after transplantation, EGFP and insulin double-positive cells were detected by immunofluorescence, EGFP and PDX1 double-positive cells were detected by fluorescence in situ hybridization (FISH) . Results Transplantation of BMSCs into pancreas reduced blood glucose,increased blood insulin and C-peptide in experimental diabetic rats, significantly differently from that of control group（P <0.05）. Up to 8 weeks after transplantation , cells of donor origin stabely expressed EGFP, and EGFP labled cells were distributed in islets (12.46%),blood vessel（4.4%）, acinus（9.24%）, and duct（3.21%）, the majority of EGFP labled cells were distributed in local focus （70.69%）. Immunofluorescence revealed double-positive cells of EGFP and insulin（5.16%）,FISH revealed double-positive cells of EGFP and PDX1（0.96%）.Conclusions Transplantation of BMSCs into pancreas effectively reversed hyperglycemia of experimental diabetes rat, transplanted BMSCs redistributed in pancreas, and transplanted BMSCs differentiated into insulin-secreting cells within pancreatic microenvironment.

Genotype-phenotype correlations in Chinese Familial adenomatous polyposis (FAP)

Wei-jia CUI; Jian-qiu SHENG; Xiao-juan LU; Lei FU; Xiao-ming MENG; Ming-zhi ZHANG

2009, 29(6):  589-592. 

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OBJECTIVE: To analyze Genotype-phenotype correlations in APC gene and Familial adenomatous polyposis (FAP) of Chinese .METHODS:APC gene germline mutation was detected in 14 FAP families . The correlations between APC gene mutations type and clinical features were synthetically analyzed. RESULTS: The patients whose mutaions were identified at codon 443,779,1062,1068,1308,1309,1394, c.657+1 and c.532-2 manifested profuse polyposis, and the patients whose large fragment deletions were detected manifested intermediate polyposis, and three patients who had not mutation or large fragment deletion detected and two of them presented intermediate polyposis and one attenuated polyposis. CONCLUSION: The range of APC gene germline mutation site in Chinese profuse polyposis wider than Western countries which report is in codon 1250~1464.

Genetic detection and prenatal diagnosis in a family with autosomal dominant polycystic kidney disease

Xiao-qiao LI; Feng-xia YAO; Yan MENG; Zheng WANG; Yuan-yuan PENG; Yan DIAN; Shang-zhi HUANG

2009, 29(6):  593-597. 

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Objective To elucidate the molecular genetic defect of autosomal dominant polycystic kidney disease in a Chinese family and to perform prenatal diagnosis for the fist pregnancy of the proband's wife. Methods Disease-causing gene was located by linkage analysis of polymorphic markers. Mutations in the proband was screened by direct sequencing the exons and the flanking introns amplified by polymerase chain reaction (PCR) . Other patients in the family were screened by allele-specific PCR (AS-PCR). Prenatal diagnosis was carried out with linkage analysis and mutation detection. Results A point mutation of c.1249C﹥T(p.R417X) in exon 5 of PKD2 gene was identified. The fetus was not to carry the mutant allele. Conclusion The mutation of c.1249C﹥T(p.R417X) was responsible for the ADPKD in this Chinese family and the prenatal diagnosis was carried out successfully.

Clone of novel gene GP1 differentially expressed in gastric cancer and its premalignant lesions

Jie ZHENG; Hai-yan ZHANG; Qing-hui YANG

2009, 29(6):  598-602. 

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Objective To explore the differentially expressed genes of gastric cancer, matched normal gastric tissue and premalignant lesions. Methods The differentially expressed cDNA bands were isolated and identified by fluorescent differential display and then reamplified by PCR.After being cloned, all cDNA fragments were sequenced. Through BLAST software, the sequencing results were compared with GenBank database for homology analysis. The expression of the cDNA fragment in different tissues was confirmed by Northern blot. SMART RACE（Rapid Amplication of cDNA Ends） was used to amplify the full length cDNA sequence. Bioinformatics analysis was performed to analysis its function. Results A differentially expressed cDNA fragment expressed lower in gastric cancers and premalignant lesions,no homology was found to known gene sequences in GenBank. The novel cDNA fragment was given an accession number of EST （CD454989）in GenBank. A full length cDNA sequence of 1362bp was acquired, who coded 267 amino acids, and was homologous with BAA91562.1, whose physiology function was unknown. Conclusion One differentially expressed gene was found and it might be involved in process of the gastric carcinogenesis and development.

Noninvasive delayed limb ischemic preconditioning decreases cerebral ischemia-reperfusion injury in rats

Qiong LUO; Heng-jie YUAN; Yan-na WU; Yi KANG; Wei-zhen GAO; Yan-xia LIU; Jian-shi LOU

2009, 29(6):  603-606. 

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Objective To study the protective effects of noninvasive delayed limb ischemic preconditioning against cerebral ischemia-reperfusion oxidative injury in rats. Method Wistar rats were divided randomly into sham operation(sham), ischemia-reperfusion(I/R), early cerebral ischemic preconditioning and ischemia-reperfusion(ECIP+I/R), and noninvasive delayed limb ischemic preconditioning and ischemia-reperfusion(NDLIP+I/R) groups. Rats in NDLIP+I/R group were subjected to three cycles of five minutes ischemia/five minutes reperfusion on the left hind limb, once a day for three consecutive days. On the forth day, all rats were subjected to cerebral ischemia/reperfusion injury. Rats　in ECIP+I/R group were preconditioned　before ischemia. Neurological score and cerebral infarct size(IS) were examined after ischemia 1h and reperfusion 24h. Activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA) were measured. Results Compared with I/R group, ECIP+I/R and NDLIP+I/R could reduce neurological score significantly after ischemia 1h and reperfusion 24h(P<0.01). Compared with I/R group, ECIP+I/R and NDLIP+I/R could reduce IS and concentration of MDA, increase total SOD（T-SOD）and Mn-SOD activity in hippocamppus and cortex(P<0.01). Among all these oxidative and antioxidative substancese, there was no significance between ECIP+I/R and NDLIP+I/R. Conclusion The protective effects of NDLIP against cerebral ischemia-reperfusion injury are similar to those of ECIP, the protective effects are associated with the increase in antioxidative ability of brain.

Investigation on 6 Genetical Traits in Yi and Bai Ethnic Groups in Guizhou Province

Qing-zhong ZHANG; Guo-qin SONG; Yue-sheng YU

2009, 29(6):  607-610. 

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Objective To study distributive frequencies and it's corelations of 6 genetical traits in Yi and Bai ethnic groups in Guizhou province. Methods According to the techniques of human population genetics and cluster random sampling, a survey on 6 genetical characters of 879 cases (Yi ethnic group 472 and Bai ethnic group 407) was carried out in October 2008，and then dealt with data using SPSS13.0 software. Results The distributive frequencies on 6 dominant traits of Yi ethnic group are 20.6％,34.3％,74.4％,76.3％,25.4％ and 60.8％ respectively; Those of Bai ethnic group are 17.2％,39.3％,64.9％,86.2％,15.5％ and 66.8％ respectively. There are very significant differences(P<0.01) between Yi and Bai ethnic groups on 3 genetical characters (eyelid, eyelash and cerumen); and significant differences(P<0.05 or P<0.01）on hair metter in sex in two ethnic groups; and significant difference（P<0.05）on two genetical characters (cerumen and Darwinian point) in sex in Bai ethnic group; Conclusions There are most people having the following characters : flat form of front hair, flexible hair matter, double eyelid, long eyelash, dry cerumen, having darwinian point and a few corelations on 6 population genetical traits in two ethnic groups in Guizhou province .

Screening of siRNAs targeted protein kinase C α and their effects on A549 cell line

Yu-xia YAN; Jian-wei JIANG; Zhi-hong HUANG; Chun-lan LIN; Xiao-ying ZHANG; Feng-yun WU

2009, 29(6):  611-617. 

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Objective To investigate effects of siRNAs targeted protein kinase C α (PKCα) on the proliferation and apoptosis in A549 cell line .Methods Six PKCα siRNAs were designed and chemical synthesized. Candicated PKCα siRNA was transfected into A549 cells, the PKCα mRNA level was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and advanced MTT assays were employed. Then, the effects of more effective PKCα siRNAs on A549 were further investigated by using clone formation assay , hoechst 33258 staining, propidium iodide (PI) staining , Annexin V-FITC and PI dual staining and western blot. Results Six candicated PKCα siRNAs were designed and named No.1, No.2, No.3, No.4, No.5, No.6 PKCα siRNA, respectively. Compared with controls, PKCα mRNA levels were all significantly downregulated and the cell proliferation were inhibited in A549 cells treated with candicated PKCα siRNAs except No.5 (P<0.05).The No.3, No.6 and No.1 PKCα siRNAs, which can more effectively inhibit A549 cell growth and PKCα expression, were identified among 6 candidate PKCα siRNAs. Compared with controls, the clone formation was significantly inhibited (P<0.05), percentages of hypodiploid cells and early apoptosis rates were significantly increased (P<0.01), and the expression of PKCα protein was significantly decreased in A549 cells treated with No.3, No.6 or No.1 PKCα siRNA (P<0.01). In addition, the typical morphologic changes of apoptosis were found in PKCα siRNA-treated A549 cells. Conclusion Three PKCα siRNAs in six candicated PKCα siRNAs can effectively downregulate PKCα protein expression, induce apoptosis and inhibit proliferation in A549 cells.

Modulation of Th1 and Th2 responses to immunization with different β-amyloid and adjuvant

Tian-tian LEI; Xue-mei ZHAO; Ping LIANG

2009, 29(6):  618-621. 

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Objective To observe stimulation and regulation of T lymphocyte subtypes when using wild-type and mutant Aβ40 and Aβ42 with different adjuvants and to investigate possible ways to lower the toxicity of Aβ immune response. Methods Forty BALB/c mice were randomized into five groups: Al adjuvant group,Aβ42+CFA group,Aβ42+Al group,Aβ40+Al group,Aβ40（E22A）+Al group. After the initial immunization and booster immunization 3 times, antibody titer were detected and the mice were killed. Their splenocytes were stimulated by respective antigen. After 48h quantity of IFN-γ, IL-2, TNF-α and IL-4 were tested. After 72h of culture, the proliferative response of splenocytes were detected by CCK-8 assay. Results Experimental groups generated specific anti-Aβ antibodies and had proliferative response of spleen lymphocytes. Cell culture supernatant of cytokine detection results showed that Aβ42+CFA group had the highest secretion of Th1-type cytokines IFN-γ,IL-2,TNF-α when Aβ40（E22A）+Al group had the lowest.(P <0.01).When Aβ40 and the mutant Aβ40+Al groups compared with Aβ42+Al group, secretion of IFN-γ,IL-2,TNF-α have also decreased significantly（P＜0.05）.The secretion of IL-4 was no significant difference. Conclusion Aβ40（E22A）+Al group stimulate the lowest toxicity of T cells, and may have better security in the immune response.

Bioprotein glue combined with gelatin sponge in prevention of epidural adhesions after operation

Yan-ming LI; Yun-Zhen CHEN; Ke-feng ZHANG; Hai-chun LIU; Bao-an PEI

2009, 29(6):  622-626. 

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Objective To approach the prophylactic function of absorbable gelatin sponge and bioprotein glue in prevention of epidural and nerve root scar adhesions after laminectomy. Methods We excised the L6 vertebral plates of 36 adult New Zealand male rabbits, to make vertebral plate excision model. Probe the nerve root. Then divided them into 3 groups according to different materials: gelatin sponge and bioproten glue (group A ), gelatin sponge ( group B ) and saline solution ( group C ). The morphological changes of the scars were observed grossly and histologically on 2 and 8 weeks after operation, and we also measured the orbit of the nerve root. Results gelatin sponge combining with bioprotein glue can reduce adhesions obviously（P＜0.05）. Conclusions gelatin sponge combining with bioprotein glue can lessen spinal dural mater and nerve root scar adhesions with surrounding tissue.

Role of PI3K/AKT signaling pathway in total hepatic ischemia -reperfusion -induced lung injury in rats

Chun-ling JIANG; Dong ZHAO; Wei-xing ZHANG

2009, 29(6):  627-630. 

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Objective To explore the role of PI3K/AKT signaling pathway in total hepatic ischemia -reperfusion (I/R)-induced lung injury in rats. Methods This study was divided into 2 parts.（1）36 rats were killed respectively at preischemia and different time after reperfusion,and the lung tissue was taken.(2)12 rats were randomly divided into Wortmannin group and model control group. AKT,p-AKT protein expression,apoptotic cells and PCNA protein expression was determined respectively by Western blot,TUNEL and immunohistochemistry analysis.Results （1）Compared with that in preiscemia group,after I/R the apoptotic index (AI)was increased,the p-AKT/AKT ratio and PCNA - positive index showed bidirectional changes，and the histological changes was severe.（2）p-AKT/AKT ratio showed a positive correlation with PCNA-positive index and a negative correlation with AI .（3）Compared with that in model control group, the p-AKT/AKT ratio and PCNA-positive index was lower, histological changes was more severe and AI was higher in Wortmannin group. Conclusion PI3K/AKT signaling pathway had protective effect on lung injury induced by total hepatic I/R ,which was maybe mediated by anti-apoptosis and promoting proliferation.

临床园地

Evaluation of several Dermatophagoides pterronyssinus Allergen extracts for Skin Prick Test

Jin-lu SUN; Rui-qi WANG; Jia YIN; Liang-lu WANG; Hong-yu ZHANG; Hong LI; Li-ping WEN

2009, 29(6):  631-635. 

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Objective To evaluate the significance of several different house dust mite allergen extracts with skin prick test (SPT) in allergic patients to Dermatophagoides pteronyssinus. Methods 219 patients underwent SPT and serum specific IgE assay to house dust mite. 3 kinds of house dust mite allergen extracts were used for SPT, respectively. Extract A group was prepared by our laboratory, extract B group was extract of Dermatophagoides pteronyssinus manufactured by ALK Corporation, and extract C group was mixed extract of Dermatophagoides pteronyssinus and Dermatophagoides farinae manufactured by ALK Corporation. Human serum specific IgE was regarded as reference of allergy to Dermatophagoides pteronyssinus. Evaluate the ROC curves of these three groups. Results 1. The medium of wheal diameter of SPT with A group, B group and C group was 0.45cm, 0.35cm and 0.30cm, respectively. 2. There was no local urticaria and systemic urticaria, no running nose and sneezing, no cough and asthma, no allergic shock observed. Local reactions after SPT were observed in 5 ( group A), 3 ( group B), 3 patients ( group C).. The delayed reactions were observed in 2 patients of each group. The area under ROC curve of SPT with extract A group, B group and C group was 0.765、0.801、0.782. The coefficient of linear correlation was 0.771, 0.786 and 0.784, respectively (P<0.01), the coefficient was statistically significant. 4. Based on serum specific IgE, the sensitivity of SPT with extract A group, B group and C group was 92.4％, 87.0％ and 81.5％, and the specificity was 60.6%, 73.2% and 74.8%, respectively. Conclusion The house dust mite allergen extract prepared in our laboratory was a proper extract for diagnosing of house dust mite allergy. The sensitivity and specificity of the extract was good, and it can be used in clinical diagnosis.

Mucormycosis in a patient with renal failure: case report and review of literature

Wen-ling YE; Peng WANG; Ying-yi WANG; Yao ZHANG; Yang YU; Hang LI; Xue-mei LI

2009, 29(6):  636-640. 

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Objective To investigate clinical characteristics, diagnosis, treatment and prognosis of mucormycosis in patients with renal failure. Mathods We reported a case of mucormycosis in a 65-year old man with chronic renal failure and treated successfully with amphotericin B. And cases of mucormycosis complicated with renal failure reported in literatures were reviewed excluding mucormycosis peritonitis associated with peritoneal dialysis and desferrioxamine-related mucormycosis. Results Fifteen cases including our case were involved with mean age of 49.87 15.84 years old. Rhinocerebral, pulmonary, and disseminated forms accounted for 46.7%, 33.3% and 20% respectively. Postmortem diagnosis was made in 46.7% of patients. Mortality rate was 73.3% in all of them and 42.9% in seven patients who received antifungal therapy. No one was survival in patients without treatments. Conclusion Mucormycosis is a lethal invasive infection in patients with renal failure. The important management to improve prognosis are early diagnosis, surgical debridement, drainage and aggressive antifungal therapy.

Multivariate Analysis for the Factors Affecting Recurrence of Aggressive Fibromatosis

Xiao-hong NING; Lin ZHAO; Ya-juan SHAO; Yu-zhou WANG

2009, 29(6):  641-644. 

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Objective To study the factors influencing the recurrence of aggressive fibromatosis(AF) patients Methods Analyze clinical features and find out factors affecting AF recurrence with univariate and multivariate analysis Results 103 pathologically diagnosed AF patients in which 67 are females were analyzed. Diameter of all the masses was 7.84±5.62cm. 98.2% of all patients received surgery and radical resection rate is 79.4%. First recurrent time after surgery of male and female is 1563±377 and 2117±3704 days respectively, it's 2723±461、657±262、2090±499、812±220、721±234 days for that of abdomen wall、head and neck(H&N), deep mass, joints and chest wall. Recurrent time is 2232±271 and 1347±267 days for those with and without locally surgery history patients. Univariate analysis reveal that gender, tumor site and surgery history of tumor site are prognostic factors of tumor recurrence. Multivariate analysis suggest that only surgery history of tumor site is an independant prognostic factors of AF recurrence after surgery. Conclusion Gender, tumor location and surgery history of tumor site can predict postsurgery recurrence of AF.

研究短文

NO donator and prosoma attenuated cerebral ischemia-reperfusion injuries in rats

Yong-zhong LIN; Chang-kai SUN; Lin SHA

2009, 29(6):  645-646. 

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Expression of adiponectin mRNA is decreased in omental adipose tissue of obese subjects in Uygur groups

Yi JIAO; Xiao-yu LIU; Yan-jiao WANG; Cheng ZHANG; Ali AKBAR; Ya-qun GUAN

2009, 29(6):  647-648. 

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短篇综述

Endoplasmic reticulum stress within hypoxia microenvironment and its related dieases

Zhao-gang DONG; Yan ZHANG; Xun QU

2009, 29(6):  649-653. 

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Endoplasmic reticulum stress, an critical component of cell stress, is a protective response for eukaryotic cells. The change of partial pressure of oxygen (for instance hypoxia) is a primary condition for cell stress, significantly impacting the biological phenotype and behavior of cells (including survival, migration and invasion et.al). Through endoplasmic reticulum stress, intracellular concentration of unfolded protein is reduced, and agglutination inhibited. Recently, endoplasmic reticulum stress has been demonstrated to play an important role in pathogenesis of some diseases such as cancer, diabetes and inflammation, which are characterized by the micro-environment hypoxia in local tissue. Therefore, investigations on the mechanism of endoplasmic reticulum stress caused by hypoxia may promote the novel therapeutic strategy for tumors, cardiovascular diseases and diabetes.

Tumor stem cell and osteosarcoma

Xing-wen HAN; Shuan-ke WANG; Xue-wen KANG

2009, 29(6):  654-656. 

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Recent research of the virulence of community-associated methicillin-resistant Staphylococcus aurues

Yu-hua ZHOU; Qing-tian LI; Xiao-kui GUO

2009, 29(6):  657-660. 

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After the emergence in 1980s, community-associated methicillin-resistant Staphylococcus aurues (CA-MRSA) has caught great attention of the researchers and clinical practitioners all over the world. In recent researching papers, people found that CA-MRSA's abilities to adapt itself to the hostile environment and to colonize is a kind of important virulence factor. A newly unveiled gene, designated arginine catabolic mobile element (ACME), in USA300 stains contributes much to this sort of ability. The arginine deiminase encoded by ACME plays an important role in colonization.

医学教育

Training of basical surgery skills for medical students at different stages

Xie-qun XU; Jin-hui WANG; Hui PAN; Ping YANG; Jian-chun YU

2009, 29(6):  661-663. 

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The staff room of Surgery of PUMCH did plenty of work to optimize the teaching model, which is characterized by stage-dependent integrity and by systematic organization as a response to the challenge on students' training.

The Evaluation System of Scientific Research Training Quanlity in the 8-year Medical Education Program

Xiao-hui GAO; Qin ZHANG; Wei-wei XU

2009, 29(6):  664-666. 

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Scientific research training program is an important part in the 8-year medical education program. It focuses on the training of scientific research thinking and research methods. It is important to establish an effective evaluation system in order to achieve the learning outcomes of scientific research training program. PUMC has established an effective evaluation system which involves research project selecting, supervising, formative assessment, oral defense. This paper introduces the evaluation system of scientific research training program at PUMC.