Abstract: Objective To construct human SREBP-1c-promoter reporter gene vector and to detect its function.　 Methods Human blood genome DNA was extracted and pGL3-Basic-SREBP-1c-promoter reporter gene vector was constructed. Furthermore, the function of SREBP-1c-promoter was confirmed by dual-luciferase reporter assay. Results　pGL3-Basic-SREBP-1c-promoter reporter gene vector was constructed successfully and the promoter activity was obviously repressed by co-transfection FoxO1. Overexpression FoxO1 inhibited the SREBP-1c protein expression. Conclusion　FoxO1 repressed the SREBP-1c protein expression by inhibition the SREBP-1c transcription.

Key words: SREBP-1c, Promoter, FoxO1, Dual-Luciferase Reporter Assay