Basic & Clinical Medicine ›› 2017, Vol. 37 ›› Issue (9): 1251-1256.
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Abstract: Objective Investigated the antitumor effects of IL-18 gene transfected T cells on pancreatic cancer SW-1990. Methods: Construction of IL-18 contained recombinant lentivirus using PCR method and packaged at HEK293T cell, then transfected the T cells and evaluated the antitumor effects when cocultured with SW-1990. Results the IL-18 gene contained recombinant lentivirus was successfully constructed and packaged, and could transfected the T cells, the LDH secretion and IL-2 and IFN-γ content were all increased significantly (P < 0.01) when cocultured with SW-1990 cell. Conclusions T cells transfected with IL-18 possessed more potent antitumor effects to pancreatic cancer SW-1990 as compared to the regular T cells.
Key words: pancreatic cancer, IL-18, T cell
CLC Number:
R392.12
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2017/V37/I9/1251