Abstract: Objective To clone and identify the core promoter of human TSLC1 used for carrying out the study of transcription regulatory mechanism. Methods A series of different fragments located in the upstream of translation start site of TSLC1 were amplified from human genomic DNA by PCR, and then constructed into pGL3-Basic luciferase reporter vector. The relative activities of different fragments in A549 and NCI-H446 cells were detected by dual-luciferase assay after transient transfection, and then the core promoter of TSLC1 was identified. Results Among the different constructs, the fragment of -68～-329bp located in the upstream of ATG showed the strong activity both in A549 cells and NCI-H446 cells, which played an important role in the transcription of TSLC1. Conclusion The fragment of -68～-329bp located in the upstream of translation start site of TSLC1 may be the core promoter region.

Key words: TSLC1, core promoter, clone, identify