Abstract: Objective　This study was to investigate the effect of nitric oxide (NO) on cell proliferation in gastric cancer cell line SGC-7901, and to investigate the relevant mechanisms. Methods MTT assay was used to detect the inhibitory effect of NO on cell growth. RT-PCR was used to detect the mRNA expression of C-FOS and C-JUN. Western blot was applied to study the protein expression of C-FOS and C-JUN. Results SNP inhibits the proliferation of SGC-7901 cells (P<0.05). SNP dose- and time-dependently decreased the expression of C-FOS, C-JUN mRNA and protein. Conclusions　NO inhibits the proliferation of SGC-7901 cells, which is probably through NO down-regulated C-FOS and C-JUN expression.

Key words: Stomach neoplasms, Nitric oxide, Cell line SGC-7901