Abstract: Objective To study the effect of atorvastatin on the interaction of endothelial cells and mesangial cells exposed to high glucose and lysophosphatidylcholine( LPC ). Methods　The model of intercellular interaction between human endothelial cells and human renal glomerular mesangial cells was established and divided into 5 groups: control, mannitol, high glucose and LPC, BN52021, atorvastatin. The level of fibronectin and PAF were determined by enzyme linked immunosorbent assay in the culture media. The expression level of platelet activating factor receptor（PAF-R）mRNA in mesangial cells was determined by real-time fluorescence quantitative PCR. Results 1.The level of fibronectin and PAF of high glucose and LPC group was higher than that of control group in co-culture and the monolayer cell culture (P<0.05 ). Intervented by high glucose and LPC, the level of fibronectin and PAF of co-culture was higher than that of the monolayer cell culture (P<0.05). The level of fibronectin of BN52021 or atorvastatin group was lower than that of high glucose and LPC group(P<0.05). The level of PAF of atorvastatin group was lower than that of high glucose and LPC group. 2. The expression level of PAF-R in mesangial cells of high glucose and LPC group was higher than that of control group in co-culture and the monolayer cell culture (P<0.05 ). Intervented by atorvastatin, the expression level of PAF-R in mesangial cells was lower than that of high glucose and LPC. Conclusions　(1) Exposed to high glucose and LPC, endothelial cells and mesangial cells can interact with each other. (2) Atorvastatin can affect the intercellular interaction between endothelial cells and mesangial cells exposed to high glucose and LPC.