Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (10): 1059-1065.

• 研究论文 • Previous Articles     Next Articles

Melatonin post-intervention could promote the expression of thioredoxin-1 in rats pancreatic tissues with acute necrotizing pancreatitis

Wei-yi ZHONG, Guo-du TANG, Zhi-hai LINAG   

  1. Guangxi Minzu Hospital Dept. Gastroenterology, the First Affiliated Hospital of Guangxi Medical University
  • Received:2010-01-25 Revised:2010-05-25 Online:2010-10-05 Published:2010-10-05
  • Contact: Guo-du TANG,

Abstract: Objective To investigate the effect of melatonin post-intervention on the expression of thioredoxin-1(TRX-1) in rats with acute necrotizing pancreatitis(ANP). Methods Male Spraque-Dawley rats were randomly allocated to control group, ANP group,melatonin post-intervention group with 24 rats in each group .The rats in ANP group received three intraperitoneal injections of 25 ml/kg body weight 6%L-arginine at an interval of 1 h to induce ANP. The rats in melatonin post-intervention group received intraperitoneal injections of 20ml/kg body weight 0.25% melatonin after ANP induction. Rats in control group received intraperitoneal injections of same amount of saline. Rats were sacrificed at 6, 12 and 24 h after ANP induction. The pathological evaluation of pancreatic tissues was performed. The concentrations of malondialdehyde and myeloperoxidase in pancreatic tissues were measured. The serum level of TRX-1 was measured by ELISA. The expressions of thioredoxin-1 and thioredoxin-1mRNA in pancreatic tissues were confirmed by immunohistochemistry and RT-PCR, respectively.Results Comparerd to the control group, pathological scores, malondialdehyde ,and myeloperoxidase of pancreatic tissues in ANP group were significantly increased(p < 0.05),while they in melatonin post-intervention group were significantly lower (p < 0.05) than those in ANP group. Comparerd to the control group,serum TRX-1 contents in ANP group significantly decreased(p < 0.05) at 6- and 12 h points, while they in melatonin post-intervention group were significantly higher (p < 0.05) than those in ANP group. the peak value of TRX-1 protein and TRX-1mRNA expressions in pancreatic tissues shifted from 12 h after ANP induction in ANP group to 6 h after ANP group in melatonin post-intervention group.Conclusions Melatonin post-intervention reduced the injury of pancreatic tissue may be through increased tissue and serum TRX-1 levels in ANP rats.

Key words: Acute necrotizing pancreatitis, melatonin, thioredoxin-1