Abstract: Objective To investigate the effect of nitric oxide (NO) on the expression of long type of leptin receptor (OB-Rb) in dorsal root ganglion (DRG) neurons cultured in vitro. Methods Sodium nitroprusside (SNP; 0, 0.01, 0.1 and 0.5 mmol/L) were used to treat the DRG neurons cultured in vitro. RT-PCR and Western Blot were used to detect the expression of long types of leptin receptor (OB-Rb) in DRG neurons. Results 0.1~0.5 mmol/L SNP can increase the expression of OB-Rb mRNA and protein Conclusion The modulation of NO on DRG neurons may be relative with the action of leptin.

Key words: 一氧化氮, 硝普钠, 瘦素受体, 背根神经节