Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (10): 1051-1054.
• 研究论文 • Previous Articles Next Articles
Chuan-yin ZHANG, Chang-e ZHANG, Ai-ping QIN, Xiao-yu LU, Chong ZHAO, Li LU
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Abstract: Objective To study Adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell (HUVEC) apoptosis. Methods The cultured HUVEC was divided into 6 groups: Control group, LPS group, dimethylsulfoxide (DMSO) group, Low, Medium and High-dose adenosine group. the cell's shape structure were observed continuously by fluorescence microscope. TNF-α expression in the supernate was determined by ELISA. Cells's apoptosis was examined by Flow Cytometry. Results The TNF-α expression and cell apoptosis were significantly increased (p<0.05); Groups of different doses of adenosine can inhibit the expression of TNF-α and cell apoptosis (p<0.05). Conclusion Certain dose of adenosine can inhibits lipopolysaccharide-induced the HUVEC release inflammation medium, adenosine can also suppress cell apoptosis.
Key words: Adenosine, LPS, HUVEC, Inflammation, Apoptosis
Chuan-yin ZHANG; Chang-e ZHANG; Ai-ping QIN; Xiao-yu LU; Chong ZHAO; Li LU. Adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell apoptosis[J]. Basic & Clinical Medicine, 2010, 30(10): 1051-1054.
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