Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (10): 1051-1054.

• 研究论文 • Previous Articles     Next Articles

Adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell apoptosis

Chuan-yin ZHANG, Chang-e ZHANG, Ai-ping QIN, Xiao-yu LU, Chong ZHAO, Li LU   

  1. Guangzhou Medical College Guangzhou Medical College
  • Received:2009-12-14 Revised:1900-01-01 Online:2010-10-05 Published:2010-10-05
  • Contact: Li LU

Abstract: Objective To study Adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell (HUVEC) apoptosis. Methods The cultured HUVEC was divided into 6 groups: Control group, LPS group, dimethylsulfoxide (DMSO) group, Low, Medium and High-dose adenosine group. the cell's shape structure were observed continuously by fluorescence microscope. TNF-α expression in the supernate was determined by ELISA. Cells's apoptosis was examined by Flow Cytometry. Results The TNF-α expression and cell apoptosis were significantly increased (p<0.05); Groups of different doses of adenosine can inhibit the expression of TNF-α and cell apoptosis (p<0.05). Conclusion Certain dose of adenosine can inhibits lipopolysaccharide-induced the HUVEC release inflammation medium, adenosine can also suppress cell apoptosis.

Key words: Adenosine, LPS, HUVEC, Inflammation, Apoptosis