Abstract: Objective To investigate whether AcSDKP can regulate MMP-2 and MMP-9 activity and MMP-1 expression in cultured rat cardiac fibroblasts mediated by 10％fetal bovine serum and PDGF. Methods The MMP-2 and MMP-9 activity was measured with zymography assays. The MMP-1 expression was detected with western blot. Results 10% fetal bovine serum （FBS） and PDGF stimulated MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts；AcSDKP further increased MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts induced by 10％ FBS and PDGF。Conclusion AcSDKP can up-regulated MMPs activity or expression of cardiac fibroblasts mediated by PDGF，which is possibly related with the effect of AcSDKP anti-fibrosis.