Abstract: Objective To observe the expressive changes of RhoA and phosphorylated myosin light chain in rat liver tissue during hepatic fibrogenesis. Methods The liver histopathological changes were evaluated by hematoxylin and eosin staining and Masson's trichrome method. Western blot was used to determine the expressions of RhoA and p-MLC(Thr18/Ser19), and reverse transcription-polymerase chain reaction (RT-PCR) to determine the expression of RhoA mRNA. Results With the development of hepatic fibrogenesis, the protein expressions of RhoA and p-MLC(Thr18/Ser19) and the mRNA expression of RhoA were significantly increased. RhoA and p-MLC(Thr18/Ser19)correlated with α-SMA positively, respectively. Conclusions Rho/ROCK signaling pathways are changed during hepatic fibrogenesis.