Protein expression and subcellular distribution of centriolar component SAS-6 in mouse oocyte meiosis

doi:10.16352/j.issn.1001-6325.2024.05.0651

Basic & Clinical Medicine ›› 2024, Vol. 44 ›› Issue (5): 651-657.doi: 10.16352/j.issn.1001-6325.2024.05.0651

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Protein expression and subcellular distribution of centriolar component SAS-6 in mouse oocyte meiosis

SONG Ke¹, SONG Ke², LI Jingyu¹, MA Wei^2*, YANG Xiaokui^1*

1. Department of Human Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University; Beijing Maternal and Child Health Care Hospital, Beijing 100026;
2. Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2024-02-26 Revised:2024-03-19 Online:2024-05-05 Published:2024-04-23
Contact: ^*mawei1026@ccmu.edu.cn;yangxiaokui@ccmu.edu.cn

Abstract

Abstract: Objective To study the expression and subcellular distribution pattern of centriolar protein SAS-6 during meiosis of mouse oocytes. Methods Immunefluorescence was conducted to analyze the subcellular distribution pattern of SAS-6 in Chinese hamster ovary cell line(CHO cell) during mitosis. Western blot was applied to detect the protein expression of SAS-6 in mouse oocyte meiosis. Immunofluorescence microscopy carried out to determine the subcellular distribution of SAS-6 and its association with microtubule organizing centers (MTOCs) and in vesicles during the oocyte meiosis. Results SAS-6 was colocalized with Pericentrin on spindle poles during the mitotic process in somatic cells. In mouse oocytes, SAS-6 was stably and consistently expressed at all stages of meiosis and specifically aggregated on GM130-positive vesicles, but not in MTOCs. The results suggested that centriolar protein SAS-6 was still expressed in oocytes lacking centrosomes, and localized in GM130-positive vesicles but expressed on percientrin-positive MTOCs. Conclusions SAS-6 may play a role in spindle cortical migration through regulating vesicles during oocyte meiosis.

Key words: spindle assembly abnormal protein 6, oocyte, microtubule organizing centers, vesicle, spindle migration

CLC Number:

R321.1

SONG Ke, SONG Ke, LI Jingyu, MA Wei, YANG Xiaokui. Protein expression and subcellular distribution of centriolar component SAS-6 in mouse oocyte meiosis[J]. Basic & Clinical Medicine, 2024, 44(5): 651-657.

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Protein expression and subcellular distribution of centriolar component SAS-6 in mouse oocyte meiosis

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