Methylglyoxal reduces cell viability and mitochondrion membrane potential of human brain microvascular endothelial cell line hCMEC/D3

doi:10.16352/j.issn.1001-6325.2023.11.1631

Basic & Clinical Medicine ›› 2023, Vol. 43 ›› Issue (11): 1631-1635.doi: 10.16352/j.issn.1001-6325.2023.11.1631

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Methylglyoxal reduces cell viability and mitochondrion membrane potential of human brain microvascular endothelial cell line hCMEC/D3

LI Tingting¹, HAN Zhenping², GAO Li¹, WANG Siyi², ZHONG Kai¹, HUANG Ping^1,3, YAN Jieping^1,3*

1. Center for Clinical Pharmacy, Department of Pharmacy, Zhejiang Provincial People's Hospital, People's Hospital Affiliated to Hangzhou Medical College, Hangzhou 310014;
2. Department of Pharmacology, College of Pharmaceutical Sciences,Zhejiang University of Technology, Hangzhou 310014;
3. Key Laboratory of Endocrine Gland Diseases of Zhejiang Province, Hangzhou 310014, China

Received:2023-02-07 Revised:2023-07-18 Online:2023-11-05 Published:2023-10-30
Contact: ^*yanjieping@hmc.edu.cn

Abstract

Abstract: Objective To explore the effects and mechanism of methylglyoxal (MGO) in human brain microvascular endothelial cell line (hCMEC/D3). Methods The hCMEC/D3 cells were incubated with MGO(0.25, 0.50, 0.75, 1.00 and 1.25 mmol/L) for 24 h. Cell viability was detected by CCK-8 assay. Cellular LDH and SOD activities were determined by commercially available kits. The change of mitochondrion membrane potential was assessed by JC-1 probe. MitoSOX probe was used to observe mitochondrial reactive oxygen species (mROS) release. Results Compared with control, cell viability of hCMEC/D3 treated with MGO decreased in a concentration-dependent manner (P＜0.01). Increased LDH activity and decreased SOD activity were observed with MGO exposure(P＜0.01). The decrease of mitochondrion membrane potential and the excessive increase of mROS were induced after MGO exposure for 12 h, which were detected by JC-1 or MitoSOX probe. Conclusions MGO reduces cell viability and mitochondria membrane potential in hCMEC/D3 cells with excessive mROS generation.

Key words: diabetic cerebral vascular complication, methylglyoxal, human brain microvascular endothelial cells line, membrane potential, mitochondrial reactive oxygen species

CLC Number:

R364.4

LI Tingting, HAN Zhenping, GAO Li, WANG Siyi, ZHONG Kai, HUANG Ping, YAN Jieping. Methylglyoxal reduces cell viability and mitochondrion membrane potential of human brain microvascular endothelial cell line hCMEC/D3[J]. Basic & Clinical Medicine, 2023, 43(11): 1631-1635.

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Methylglyoxal reduces cell viability and mitochondrion membrane potential of human brain microvascular endothelial cell line hCMEC/D3

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