关键词: 宫颈癌, 基质辅助激光解析离子化飞行时间质谱, 纳米磁珠

Abstract: Objective To analyze the serum proteomic pattern of the cervical cancer patients，build diagnostic model and evaluate its clinic significance. Methods WCX magnetic bead and MALDI-TOF were used to detect the serum proteomic pattern of 77 patients with cervical squanmous cell carcinomas, 13 patients with CINⅢ and 52 healthy women. Biomarker Wizard software was used to detect protein peaks significantly different between cervical cancer and controls. The model was built by Biomarker Patterns software which was further valuated in blind test. Results A diagnostic pattern consisting of three differential protein peaks was established with 100%（32/32）sensitivity and 93.8%（30/32）specificity. A sensitivity of 77.8%（35/45）and a specificity of 75%（15/20）in blind test were obtained. The diagnostic model also could discriminate CINⅢ and SCC-Ag negative patients from controls. Conclusions The diagnostic pattern combining 3974，3398，13732m/z protein peaks can discriminate not only cervical squamous cell cancer but also CINⅢ and SCC-Ag negative patients from controls.

Key words: Cervical cancer, MALDI-TOF-MS, magnetic bead