Abstract: Objective To isolate and culture meshenchymal stem cells from murine fetal liver in vitro. Methods flMSCs from mouse fetuses were isolated by modified adhering to plastic. Growth kinetics was determined by growth curve. Cell cycle and phenotype were analyzed by FACSan flow cytometry. Differentiation of adhering cells was induced and identified in vitro. Results Homogenous fibroblast-like cells were predominated in culture. The number of flMSCs increased 2 fold after about 24 hours. 83.76%±2.88% of flMSCs stayed in the G0/G1 phases. flMSCs were CD44, CD29 positive but negative for the markers of hematopoietic cells such as CD45, CD11b. flMSCs were able to differentiate along adipogenic, chondrogenic and osteogenic pathways even after being passaged several times. Conclusions flMSCs can be isolated by their plastic-attachable property. flMSCs can be greatly expanded without losing their multiple differentiation potential in vitro. flMSCs could be an appropriate source of stem cell therapy.