Tim-3/galectin-9调控脂多糖诱导的人淋巴细胞中Th1/Th2细胞平衡

doi:10.16352/j.issn.1001-6325.2023.10.1542

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (10): 1542-1548.doi: 10.16352/j.issn.1001-6325.2023.10.1542

Tim-3/galectin-9调控脂多糖诱导的人淋巴细胞中Th1/Th2细胞平衡

孙晓丽¹, 张进进¹, 宋凤英², 黄永莉¹, 陈立力¹, 邢颜超^1*

1.新疆军区总医院输血科,新疆乌鲁木齐 830000;
2.新疆维吾尔自治区塔城地区裕民县人民医院检验科,新疆裕民 834700

收稿日期:2023-03-13 修回日期:2023-07-24 出版日期:2023-10-05 发布日期:2023-10-05
通讯作者: *xingyanchao@aliyun.com
基金资助:
新疆维吾尔自治区自然科学基金青年科学基金(2022D01C843)

Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes

SUN Xiaoli¹, ZHANG Jinjin¹, SONG Fengying², HUANG Yongli¹, CHEN Lili¹, XING Yanchao^1*

1. Department of Blood Transfusion, General Hospital of Xinjiang Military Command, Urumqi 830000;
2. Department of Clinical Laboratory, Yumin County People's Hospital, Yumin 834700,China

Received:2023-03-13 Revised:2023-07-24 Online:2023-10-05 Published:2023-10-05
Contact: *xingyanchao@aliyun.com

摘要/Abstract

摘要： 目的探讨T细胞免疫球蛋白黏蛋白分子-3(Tim-3)/半乳糖凝集素-9(galectin-9)在脂多糖(LPS)诱导淋巴细胞炎性过程中对辅助性T细胞(Th)Th1/Th2细胞失衡中的作用。方法收集脓毒症患者的外周血。分离外周血单个核细胞(PBMC),RT-qPCR检测Tim-3、galectin-9、T-bet和GATA3的表达;ELISA检测血清sTim-3、galectin-9、IFN-γ和IL-4的水平;分离的外周血单个核细胞体外培养,LPS刺激建立体外炎性模型;ELISA法检测细胞培养上清中Th1和Th2细胞相关细胞因子的水平;Western blot检测JAK2/STAT3相关因子的表达;Pearson相关系数分析Tim-3、galectin-9、IFN-γ和IL-4的相关性。结果脓毒症患者外周血中Tim-3和galectin-9的mRNA表达增多,sTim-3、galectin-9、IL-4和GATA-3升高,IFN-γ和T-bet水平下降,磷酸化JAK2和STAT3升高(P＜0.05)。阻断Tim-3表达后,IFN-γ水平升高,而IL-4的水平明显下降,磷酸化JAK2和STAT3下降(P＜0.05)。Tim-3、IL-4与galectin-9呈正相关,而Tim-3与IFN-γ呈负相关,IFN-γ与galectin-9呈负相关,Tim-3与IL-4呈正相关。结论在LPS诱导的炎性细胞中,Tim-3/galectin-9可能调控JAK2/STAT3通路,导致Th1/Th2细胞的失衡。

关键词: T细胞免疫球蛋白黏蛋白分子-3, 半乳糖凝集素-9, 辅助性T细胞, JAK2/STAT3通路, 脓毒症

Abstract: Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation.

Key words: Tim-3, galectin-9, T helper cells, JAK2/STAT3 pathway, sepsis

孙晓丽, 张进进, 宋凤英, 黄永莉, 陈立力, 邢颜超. Tim-3/galectin-9调控脂多糖诱导的人淋巴细胞中Th1/Th2细胞平衡[J]. 基础医学与临床, 2023, 43(10): 1542-1548.

SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao. Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes[J]. Basic & Clinical Medicine, 2023, 43(10): 1542-1548.

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Tim-3/galectin-9调控脂多糖诱导的人淋巴细胞中Th1/Th2细胞平衡

Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes

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