长链非编码RNA MCM3AP-AS1靶向调控miR-876-5p促进人结直肠癌细胞系的增殖和迁移

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (2): 225-232.

长链非编码RNA MCM3AP-AS1靶向调控miR-876-5p促进人结直肠癌细胞系的增殖和迁移

周堤侠, 吕海栋^*

青海省人民医院肿瘤外科, 青海西宁 810007

收稿日期:2019-10-28 修回日期:2020-05-30 出版日期:2021-02-05 发布日期:2021-01-19
通讯作者: ^*lvhaid@126.com

Long non-coding RNA MCM3AP-AS1 targeted miR-876-5p promotes the proliferation and migration of colorectal cancer cell lines

ZHOU Di-xia, LYU Hai-dong^*

Department of Tumor Surgery, Qinghai Provincial People's Hospital, Xining 810007, China

Received:2019-10-28 Revised:2020-05-30 Online:2021-02-05 Published:2021-01-19
Contact: ^*lvhaid@126.com

摘要/Abstract

摘要： 目的探讨长链非编码RNA(lncRNA)微小染色体维持蛋白3相关蛋白-反义链1(MCM3AP-AS1)靶向调控miR-876-5p对结直肠癌(CRC)细胞的增殖和迁移的作用。方法实时定量聚合酶链式反应法(RT-qPCR)检测CRC组织和细胞中MCM3AP-AS1的表达;用MTT法、划痕愈合实验和Transwell小室法检测CRC细胞的增殖和迁移;采用生物信息分析、双荧光素酶基因报告实验、RT-qPCR验证MCM3AP-AS1和miR-876-5p的靶向关系。结果与非肿瘤组织和正常结直肠上皮细胞系相比,MCM3AP-AS1在CRC组织和细胞系中表达上调(P＜0.05);敲低MCM3AP-AS1能抑制CRC细胞增殖和迁移(P＜0.05);生物信息学分析、双荧光素酶基因报告实验和RT-qPCR表明MCM3AP-AS1与miR-876-5p可相互作用;miR-876-5p可削弱MCM3AP-AS1对CRC细胞增殖和迁移的促进作用(P＜0.05)。结论 MCM3AP-AS1可以通过吸附miR-876-5p促进CRC细胞的增殖和迁移。

关键词: MCM3AP-AS1, miR-876-5p, 结直肠癌, 增殖, 迁移

Abstract: Objective To investigate the effect and mechanism of long non-coding RNA MCM3AP antisense transcript 1 and miR-876-5p on the proliferation and migration of colorectal cancer(CRC) cells in vitro. Methods The expressions of MCM3AP-AS1 in CRC tissue and cells were detected by real time quantitative polymerase chain reaction (RT-qPCR). The proliferation and migration of CRC cells were detected by MTT, scratch test and Transwell assay; and the targeting relationship between MCM3AP-AS1 and miR-876-5p was verified by bioinformatics analysis, luciferase assay and RT-qPCR. Results Compared with normal adjacent tissues and normal CRC epithelial cell lines, the expression of MCM3AP-AS1 was up-regulated. Down-regulation of MCM3AP-AS1 decreased the proliferation and migration of colorectal cancer cells. MCM3AP-AS1 targeted at miR-876-5p and down-regulated its expression. miR-876-5p inhibited the promotion of malignant phenotype of colon cancer. Conclusions MCM3AP-AS1 may promote the proliferation and migration of CRC cancer cells by absorbing of miR-876-5p.

Key words: MCM3AP-AS1, miR-876-5p, colorectal cancer, proliferation, migration

中图分类号:

R735

周堤侠, 吕海栋. 长链非编码RNA MCM3AP-AS1靶向调控miR-876-5p促进人结直肠癌细胞系的增殖和迁移[J]. 基础医学与临床, 2021, 41(2): 225-232.

ZHOU Di-xia, LYU Hai-dong. Long non-coding RNA MCM3AP-AS1 targeted miR-876-5p promotes the proliferation and migration of colorectal cancer cell lines[J]. Basic & Clinical Medicine, 2021, 41(2): 225-232.

参考文献

[1] Tenesa A, Dunlop MG. New insights into the aetiology of colorectal cancer from genome-wide association studies[J]. Nat Rev Genet, 2009, 10: 353-358.
[2] Howe HL, Wu X, Ries LA, et al. Annual report to the nation on the status of cancer, 1975-2003, featuring cancer among U.S. Hispanic/Latino populations[J]. Cancer, 2006, 107: 1711-1742.
[3] Ørom UA, Shiekhattar R. Long non-coding RNAs and enhancers[J]. Curr Opin Genet Dev, 2011, 21: 194-198.
[4] Wang Y, Yang L, Chen T, et al. A novel MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis[J]. Mol Cancer, 2019, 18:28. doi: 10.1186/s12943-019-0957-7.
[5] Yang C, Zheng J, Xue Y, et al. The effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 axis regulating glioblastoma angiogenesis[J]. Front Mol Neurosci, 2018, 10:437. doi: 10.3389/fnmol.2017.00437. eCollection 2017.
[6] Bartel DP. microRNAs: genomics, biogenesis, mechan-ism, and function[J]. Cell, 2004, 116: 281-297.
[7] Xu J, Zheng J, Wang J, et al. miR-876-5p suppresses breast cancer progression through targeting TFAP2A[J]. Exp Ther Med, 2019, 18: 1458-1464.
[8] Xu Z, Yu Z, Tan Q, et al. miR-876-5p regulates gastric cancer cell proliferation, apoptosis and migration through targeting WNT5A and MITF[J]. Biosci Rep,2019, 39.doi: 10.1042/BSR20190066.
[9] Tang J, Yan T, Bao Y, et al. LncRNA GLCC1 promotes colorectal carcinogenesis and glucose metabolism by stabilizing c-Myc[J]. Nat Commun, 2019, 10: 3499. doi: 10.1038/s41467-019-11447-8.
[10] Jia GQ, Zhang MM, Wang K, et al. Long non-coding RNA PLncRNA-1 promotes cell proliferation and hepatic metastasis in colorectal cancer[J]. J Cell Biochem,2018, 119: 7091-7104.
[11] Lin M, Zhang Z, Gao M, et al. microRNA-193a-3p suppresses the colorectal cancer cell proliferation and progression through downregulating the PLAU expression[J]. Cancer Manag Res, 2019, 11: 5353-5363.
[12] Chou J, Wang B, Zheng T, et al. MALAT1 induced migration and invasion of human breast cancer cells by competitivelymbinding miR-1 with cdc42[J]. Biochem Biophys Res Commun, 2016, 472: 262-269.
[13] Liang M, Jia J, Chen L, et al. MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer[J]. Endocrine, 2019, 65: 318-326.
[14] Zhi Y, Abudoureyimu M, Zhou H, et al. FOXM1-mediated linc-ROR regulates the proliferation and sensi-tivity to sorafenib in hepatocellular carcinoma[J]. Mol Ther Nucleic Acids, 2019, 16: 576-588.
[15] Liu L, Wang HJ, Meng T, et al. LncRNA GAS5 inhibits cell migration and invasion and promotes autophagy by targeting miR-222-3p via the GAS5/PTEN-signaling pathway in CRC[J]. Mol Ther Nucleic Acids, 2019, 17: 644-656.