miR-141通过靶向下调EphA2表达而抑制口腔鳞状细胞癌细胞系的增殖

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (2): 213-217.

miR-141通过靶向下调EphA2表达而抑制口腔鳞状细胞癌细胞系的增殖

韩哲¹,李磊²,周晓清²

1. 天津医科大学第二医院
2. 济宁市第一人民医院

收稿日期:2014-06-05 修回日期:2014-09-25 出版日期:2015-02-05 发布日期:2015-01-23
通讯作者: 李磊 E-mail:zxq_wyr@163.com

miR-141 suppresses cell proliferation by targeting EphA2 in human oral squamous cell carcinoma

Received:2014-06-05 Revised:2014-09-25 Online:2015-02-05 Published:2015-01-23

摘要/Abstract

摘要： 目的探讨miR-141对口腔鳞状细胞癌（OSCC）CAL27细胞增殖活性的影响及其与EphA2的靶向关系。方法构建pre-miR-141、EphA2野生型（EphA2-WT）和突变型（EphA2-MT）真核表达载体，采用实时定量PCR（qRT-PCR）检测pre-miR-141的转染效率。用MTT检测细胞增殖活性，用双荧光素酶报告基因检测、qRT-PCR和Western blot验证miR-141与EphA2的靶向关系。结果 pcDNATM6.2-GW-pre-miR-141、pmirGLO-EphA2-WT和pmirGLO-EphA2-MT表达载体构建成功，转染pre-miR-141的CAL27细胞miR-141的表达显著升高（P＜0.001）。miR-141过表达能够明显抑制CAL27细胞的增殖（P＜0.05），共转染pre-miR-141和EphA2-WT的CAL 27细胞其荧光活性显著下降（P＜0.001），转染pre-miR-141的CAL27细胞其EphA2的表达要显著低于转染空质粒组（P＜0.001），miR-141的过表达能够显著抑制EphA2的蛋白表达水平。结论 miR-141的过表达可能通过靶向下调EphA2的表达从而抑制OSCC的增殖。

关键词: 口腔鳞状细胞癌, miR-141, EphA2, 增殖

Abstract: Objective To assess the effect of miR-141 on proliferation of human oral squamous cell carcinoma and target relationship between miR-141 and EphA2. Methods pcDNATM6.2-GW-pre-miR-141 was constructed and identified by qRT-PCR. EphA2-WT and EphA2-MT sequences were respectively cloned into pmirGLO plasmid. The potential proliferation function of miR-141 on CAL27 cells was analyzed by MTT. The target relationship between miR-141 and EphA2 was identified by Dual-Luciferase Assay System, qRT-PCR and Western blot. Results We constructed successfully the recombinant plasmids, including pcDNATM6.2-GW-pre-miR-141, pmirGLO-EphA2-WT and pmirGLO-EphA2-MT, and the transfection efficiency of pre-miR-141 was increased in CAL27 cells compared to control group(P＜0.001). miR-141 could suppress the proliferation of CAL27 cells(P＜0.05). Furthermore, a significant reduction of luciferase activities of CAL27 cells co-transfected with pre-miR-141 and EphA2-WT(P＜0.001). The mRNA(P＜0.001) and protein expression levels of EphA2 were decreased in CAL 27 cells transfected with pre-miR-141. Conclusion Overexpression of miR-141 could suppress cell proliferation by targeting EphA2 in CAL27 cells.

Key words: oral squamous cell carcinoma, miR-141, EphA2, proliferation

韩哲李磊周晓清. miR-141通过靶向下调EphA2表达而抑制口腔鳞状细胞癌细胞系的增殖[J]. 基础医学与临床, 2015, 35(2): 213-217.

[1]	许佐明谭川路远姚天尉吴贤建汪建初浦涧. 蛋白激酶B通过调控低氧诱导因子-1表达干预人肝癌细胞系SMMC-7721增殖[J]. 基础医学与临床, 2019, 39(3): 360-364.
[2]	高利超王梦平李雪欧三桃吴蔚桦刘建. 生松素减轻大鼠糖尿病肾病[J]. 基础医学与临床, 2019, 39(2): 165-169.
[3]	刘桂元张永慧庞毅. WNT信号抑制因子DKK3基因对结直肠癌细胞系HCT116迁移与侵袭的影响[J]. 基础医学与临床, 2019, 39(10): 1437-1443.
[4]	周小芬蒋光愉杨会琴. 下调胰岛素样生长因子1 受体抑制视网膜母细胞瘤细胞增殖和促进凋亡[J]. 基础医学与临床, 2019, 39(1): 32-36.
[5]	蔺茹君李思源肖智伟赵丽颖赵梦瑶赵晓伟李军. 塞来昔布抑制食管癌细胞系ECa109增殖[J]. 基础医学与临床, 2019, 39(1): 88-89.
[6]	侯丛丛马晓骊陈虹黄秉仁陈等. YAF2靶向cyclin D1表达促进肿瘤细胞增殖[J]. 基础医学与临床, 2018, 38(6): 764-770.
[7]	蔡惠美曹文瑜黄玉钿傅建英马燕燕. 高迁移率族蛋白1促进原发性肝癌细胞增殖[J]. 基础医学与临床, 2018, 38(10): 1417-1421.
[8]	李春艳童安莉王芬崔云英闫朝丽. 辛伐他汀对血管紧张素II刺激的肾上腺皮质癌H295R细胞的分泌和增殖的影响[J]. 基础医学与临床, 2017, 37(3): 346-350.
[9]	李鸿张嘉越李松明冷津立李瑞何辉庞飞雄. 碘酸钾对人甲状腺癌细胞系周期的影响[J]. 基础医学与临床, 2017, 37(12): 1724-1728.
[10]	黄小环封玉玲周铁军侯良绢. siRNA沉默COX-2基因抑制MDA-MB-231细胞增殖[J]. 基础医学与临床, 2017, 37(11): 1620-1621.
[11]	石雪萍李晓朋熊伟李海星郭珮王芬陈地龙李静. 吴茱萸碱抑制荷瘤小鼠结肠癌HCT-116细胞增殖[J]. 基础医学与临床, 2017, 37(10): 1373-1377.
[12]	徐婷刘北忠肖春兰单志灵淦柳根杨蓉李浏宋浩钟梁. 由ATRA诱导表达的Sp100对白血病NB4细胞增殖的影响[J]. 基础医学与临床, 2017, 37(1): 8-12.
[13]	赵灵林曾盛源王洋洋段昌柱. UHRF2在羟基脲所致的DNA损伤应答中促进H3K9乙酰化抑制细胞增殖[J]. 基础医学与临床, 2016, 36(9): 1206-1210.
[14]	朱平莫小梅刘智敏. 镉通过GPER1-ERK/AKT通路促进甲状腺癌WRO细胞系增殖[J]. 基础医学与临床, 2016, 36(7): 918-923.
[15]	温馨翟頔王芳余佳. miR-551b-3p在胃癌组织中低表达并抑制胃癌细胞系HGC-27增殖、迁移和侵袭[J]. 基础医学与临床, 2016, 36(5): 610-614.