β-catenin过表达对骨肉瘤细胞TE85迁移、侵袭及凋亡的影响

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (11): 1491-1496.

β-catenin过表达对骨肉瘤细胞TE85迁移、侵袭及凋亡的影响

罗光金¹,康权²,毕杨²,迭小红²,刘敏²,仇超²,罗庆³

1. 重庆医科大学附属儿童医院905干细胞室
2. 重庆医科大学附属儿童医院
3. 重庆医科大学附属儿童医院儿科研究所外科实验室

收稿日期:2014-06-20 修回日期:2014-09-25 出版日期:2014-11-05 发布日期:2014-11-03
通讯作者: 罗庆 E-mail:gaihuan168@163.com
基金资助:
国家自然科学基金;重庆市科委项目;重庆市卫生局重点项目

Effects of β-catenin on cell migration、invasion and apoptosis of TE85 osteosarcoma cells

Received:2014-06-20 Revised:2014-09-25 Online:2014-11-05 Published:2014-11-03

摘要/Abstract

摘要： 目的探讨β-catenin对骨肉瘤细胞TE85迁移、侵袭及凋亡的影响。方法 RT-PCR及Western blot检测β-catenin在不同骨肉瘤细胞中的表达情况，重组腺病毒Adβ-catenin感染TE85细胞，RT-PCR及荧光素酶报告基因检测感染Adβ-catenin后β-catenin mRNA的表达及活性，划痕实验检测迁移能力，Transwell实验检测侵袭能力，DAPI染色和流式细胞技术检测细胞凋亡情况。结果 TE85细胞内源性β-catenin表达相对较低(P<0.05)，Adβ-catenin能显著增加TE85细胞外源性β-catenin基因表达及活性，β-catenin组细胞迁移能力明显减慢(P<0.05),β-catenin组侵袭能力明显低于对照组(P<0.05)。结论 β-catenin能抑制肿瘤细胞TE85的迁移及侵袭能力，对凋亡无影响。

关键词: 关键词：β-catenin, TE85细胞, 迁移, 侵袭, 凋亡

Abstract: Objective To investigate the effect of β-catenin on the migration, invasion and apoptosis of osteosarcoma cell line TE85. Methods The mRNA and protein expression of total β-catenin in different osteosarcoma cell lines were detected by RT-PCR and Western blot, and the osteosarcoma cell line TE85 was infected with Adβ-catenin, then RT-PCR and the reporter TOP-Luc were used to determine the expression of β-catenin and the the β-catenin/TCF4 activity of TE85 cells.The cell migration and invasion were detected by scratch wound assay, and Transwell, respectively. DAPI staining and FCM were performed to detect cell apoptosis. Results The endogenous expression of β-catenin of TE85 cells was relatively lower in osteosarcoma cell line compared with other osteosarcoma cells. The mRNA expression level of β-catenin was increased after infection, as well as the activity of β-catenin/TCF4. Scratch test and Transwell results showed that the migration and invasion capacity of cells significantly decreased after Adβ-catenin infection (P<0.05). However, the overexpression of β-catenin resulted in no significant changes in cell apoptosis. Conclusion β-catenin can effectively inhibit the migration, invasion ability of the TE85 cells, but does not affect on its apoptosis. Our findings suggest that β-catenin may be providing a new target for the treatment of osteosarcoma.

Key words: Key words：β-catenin, TE85, Migration, Invasion, Apoptosis

中图分类号:

R738.1

罗光金康权毕杨迭小红刘敏仇超罗庆. β-catenin过表达对骨肉瘤细胞TE85迁移、侵袭及凋亡的影响[J]. 基础医学与临床, 2014, 34(11): 1491-1496.

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