关键词: miR-195, T98G细胞, 细胞增殖, 凋亡, BCL-2

Abstract: Abstract：Objective To investigate the influence of overexpression of miR-195 on cell proliferation in glioma T98G cells and explore the function of miR-195 in glioma. Method T98G cells containing lower expression level of miR-195 were transfected with synthesized miR-195 mimics , then MTT assay, flow cytometry and TUNEL assay were performed to analysis the alterations of cellular phenotype，western blot was performed to examine the expression level of BCL-2. Result Mature mir-195 increased significantly after miR-195 mimics transfection (P<0.05). Overexpression of miR-195 induced significant cell proliferation suppression. Flow cytometry results showed that up-regulated miR-195 produced little influence on cell cycle, while TUNEL assay indicated that miR-195 promoted apoptosis in T98G cells. Western blot result showed that BCL-2 was reduced after by miR-195 mimics. Conclusion Overexpression of miR-195 significantly promoted cell apoptosis in T98G cells, suggesting that miR-195 may function in glioma treatment.

Key words: miR-195, T98G cells, cell proliferation, apoptosis, BCL-2