LncRNA-ATB抑制人瘢痕疙瘩成纤维细胞的增殖和胶原合成

doi:10.16352/j.issn.1001-6325.2022.10.1543

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1543-1548.doi: 10.16352/j.issn.1001-6325.2022.10.1543

LncRNA-ATB抑制人瘢痕疙瘩成纤维细胞的增殖和胶原合成

吕雄^1,2, 仇灵江³, 劳洵姬², 谈伟强^1*

1.浙江大学医学院附属邵逸夫医院整形外科,浙江杭州 310020;
2.温州医科大学附属衢州医院(衢州市人民医院)医疗美容科, 浙江衢州 324000;
3.浙江省台州医院整形美容科,浙江台州 317099

收稿日期:2021-11-01 修回日期:2022-06-28 出版日期:2022-10-05 发布日期:2022-09-23
通讯作者: * tanweixxxx@163.com
基金资助:
浙江省市县级科技计划项目(21ywa10)

LncRNA-ATB inhibits human keloid fibroblast proliferation and collagen synthesis

LYU Xiong^1,2, QIU Ling-jiang³, LAO Xun-ji², TAN Wei-qiang^1*

1. Department of Plastic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine,Hangzhou 310020;
2. Department of Medical Cosmetology,The Quzhou Affiliated Hospital of Wenzhou Medical University,Quzhou People's Hospital,Quzhou 324000;
3. Department of Plastic Surgery,Taizhou Hospital,Taizhou 317099, China

Received:2021-11-01 Revised:2022-06-28 Online:2022-10-05 Published:2022-09-23
Contact: * tanweixxxx@163.com

摘要/Abstract

摘要： 目的探讨lncRNA-ATB对人瘢痕疙瘩成纤维细胞(KFB)增殖和胶原合成的影响及其可能的分子机制。方法体外分离培养人KFB和正常成纤维细胞(NFB),将KFB分为NC组、si-NC组、si-lncRNA-ATB组、si-lncRNA-ATB+TNF-α组;RT-qPCR检测NFB和KFB中lncRNA-ATB表达水平;MTT检测细胞活力;流式细胞仪检测细胞周期;Western blot检测cyclinD1、胶原合成和NF-κB信号通路相关蛋白的表达。结果与NFB比较,KFB细胞中lncRNA-ATB表达水平显著升高(P＜0.05);敲减lncRNA-ATB表达降低了KFB细胞活力、cyclinD1表达水平以及S期细胞百分比,升高了G₀/G₁期细胞百分比(P＜0.05);降低了胶原蛋白Col-Ⅰ和Col-Ⅲ的表达水平(P＜0.05);降低了NF-κB信号通路相关蛋白p-p65和p-IκBα的表达水平(P＜0.05);NF-κB活化激活剂TNF-α减轻敲减lncRNA-ATB表达对KFB细胞增殖和胶原合成的抑制作用(P＜0.05)。结论敲减lncRNA-ATB表达可能通过下调NF-κB信号通路抑制KFB的增殖和胶原合成。

关键词: lncRNA-ATB, NF-κB信号通路, 增殖, 胶原合成

Abstract: Objective To investigate the effects of lncRNA-ATB on the proliferation and collagen synthesis of human keloid fibroblast (KFB) and its possible molecular mechanism. Methods Human KFB and normal fibroblasts (NFB) were isolated and cultured in vitro, KFB was divided into NC group, si-NC group, si-lncRNA-ATB group, si-lncRNA-ATB+TNF-α group. RT-qPCR was used to detect the expression of lncRNA-ATB in NFB and KFB. MTT was used to detect cell activity. Flow cytometry was used to detect cell cycle. Western blot was used to detect expression of cyclinD1, collagen synthesis and NF-κB signaling pathway related protein. Results The expression of lncRNA-ATB in KFB cells was significantly increased (P＜0.05). Knock-down lncRNA-ATB expression decreased the A value of KFB cells, the expression of cyclinD1 and the percentageof S phase cells,but increased the percent- age of G₀/G₁ phase cells (P＜0.05); the expression of Col-Ⅰ and Col-Ⅲ of collagen was decreased(P＜0.05); the expression of p-p65 and p-IκBα in NF-κB signaling pathway was inhibited(P＜0.05); NF-κB activation activator TNF-α alleviated the inhibitory effect of knock-down lncRNA-ATB expression on KFB cell proliferation and collagen synthesis (P＜0.05). Conclusions Knock-down of lncRNA-ATB expression may inhibit KFB proliferation and collagen synthesis by down-regulating NF-κB signaling pathway.

Key words: lncRNA-ATB, NF-κB signaling pathway, proliferation, collagen pathway

中图分类号:

R622

吕雄, 仇灵江, 劳洵姬, 谈伟强. LncRNA-ATB抑制人瘢痕疙瘩成纤维细胞的增殖和胶原合成[J]. 基础医学与临床, 2022, 42(10): 1543-1548.

LYU Xiong, QIU Ling-jiang, LAO Xun-ji, TAN Wei-qiang. LncRNA-ATB inhibits human keloid fibroblast proliferation and collagen synthesis[J]. Basic & Clinical Medicine, 2022, 42(10): 1543-1548.

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LncRNA-ATB抑制人瘢痕疙瘩成纤维细胞的增殖和胶原合成

LncRNA-ATB inhibits human keloid fibroblast proliferation and collagen synthesis

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