花姜酮通过上调FBXW7表达抑制人非小细胞肺癌细胞系A549增殖、迁移和侵袭

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (8): 1163-1168.

花姜酮通过上调FBXW7表达抑制人非小细胞肺癌细胞系A549增殖、迁移和侵袭

万雪琼^1*, 陈佳², 何超³, 叶星明⁴

湖南省胸科医院1.综合科;2.内五科;3.胸外科;4.内二科, 湖南长沙 410006

收稿日期:2020-07-02 修回日期:2020-12-20 出版日期:2021-08-05 发布日期:2021-07-21
通讯作者: *45000193@qq.com

Zerumbone inhibits the proliferation, migration and invasion of human non-small cell lung cancer cell line A549 through up-regulating FBXW7 expression

WAN Xue-qiong^1*, CHEN Jia², HE Chao³, YE Xing-ming⁴

1. General Department; 2.Fifth Department of Internal Medicine; 3. Department of Thoracic Surgery;4. Second Department of Internal Medicine, Hunan Chest Hospital, Changsha 410006, China

Received:2020-07-02 Revised:2020-12-20 Online:2021-08-05 Published:2021-07-21
Contact: *45000193@qq.com

摘要/Abstract

摘要： 目的探讨花姜酮对非小细胞肺癌细胞系增殖、迁移和侵袭的影响及其潜在机制。方法将人非小细胞肺癌细胞系A549分为对照组、花姜酮-低剂量组、花姜酮-中剂量组、花姜酮-高剂量组、pcDNA组、pcDNA-FBXW7组、花姜酮-高剂量+si-NC组、花姜酮-高剂量+si-FBXW7组。四甲基偶氮唑蓝(MTT)法检测细胞增殖抑制率,Transwell小室法检测细胞迁移和侵袭,RT-qPCR和Western blot检测F-BOX家族F框/WD-40域蛋白7(FBXW7)mRNA和蛋白的表达。结果与对照组比较,花姜酮-低、中、高剂量组A549细胞增殖、迁移和侵袭能力显著降低(P＜0.05),FBXW7 mRNA和蛋白表达显著升高(P＜0.05)。与pcDNA组比较,pcDNA-FBXW7组A549细胞增殖、迁移和侵袭能力显著降低(P＜0.05)。与花姜酮-高剂量+si-NC组比较,花姜酮-高剂量+si-FBXW7组A549细胞系增殖、迁移和侵袭能力显著升高(P＜0.05)。结论花姜酮可降低非小细胞肺癌细胞系的增殖、迁移和侵袭能力,其机制与上调FBXW7表达有关。

关键词: 花姜酮, 非小细胞肺癌, 增殖, 迁移侵袭, FBXW7

Abstract: Objective To explore the effect of zerumbone on proliferation, migration and invasion of non-small cell lung cancer cell line and underline mechanism. Methods Human non-small cell lung cancer cells line A549 was divided into control group, zingerone-low dose group, zingerone-medium dose group, zingerone-high dose group, pcDNA group, pcDNA-FBXW7 group, and zingerone-high dose group +si-NC group, zingerone-high dose +si-FBXW7 group. Cell proliferation inhibition was detected by methyl thiazolyl tetrazolium (MTT) method, cell migration and invasion were detected by Transwell assays, and the expression of F-box with 7 tandem WD40 (FBXW7) mRNA and protein were detected by Western blot and real-time(RT-qPCR). Results Compared with control group, the proliferation, migration and invasion of A549 cells in the zingerone-low, medium and high dose groups were significantly reduced (P＜0.05), the expression of FBXW7 mRNA and protein were significantly increased (P＜0.05). Compared with the pcDNA group, the proliferation, migration and invasion of A549 cells in the pcDNA-FBXW7 group were significantly reduced (P＜0.05). Compared with the Zingerone-high-dose+si-NC group, the cell proliferation, migration and invasion ability of A549 in the Zingerone-high-dose+si-FBXW7 group was significantly increased (P＜0.05). Conclusions Zerumbone reduces proliferation, migration and invasion of non-small cell lung cancer cells, and the underlying mechanism is related to the up-regulation of FBXW7 expression.

Key words: zerumbone, non-small cell lung cancer, proliferation, migration and invasion, FBXW7

中图分类号:

R734.2

万雪琼, 陈佳, 何超, 叶星明. 花姜酮通过上调FBXW7表达抑制人非小细胞肺癌细胞系A549增殖、迁移和侵袭[J]. 基础医学与临床, 2021, 41(8): 1163-1168.

WAN Xue-qiong, CHEN Jia, HE Chao, YE Xing-ming. Zerumbone inhibits the proliferation, migration and invasion of human non-small cell lung cancer cell line A549 through up-regulating FBXW7 expression[J]. Basic & Clinical Medicine, 2021, 41(8): 1163-1168.

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