基础医学与临床 ›› 2021, Vol. 41 ›› Issue (7): 970-974.

• 研究论文 • 上一篇    下一篇

阿胶对慢性阻塞性肺疾病大鼠肺功能及肺组织病理损伤的影响

那扎开提·艾尼瓦尔1,胡广2,张田甜3,宋美月4,赵红梅3,靳洪涛2,王婧3   

  1. 1. 中国医学科学院基础医学研究所北京协和医学院基础学院病理生理学系
    2. 中国医学科学院药物研究所
    3. 中国医学科学院基础医学研究所
    4. 北京中医药大学
  • 收稿日期:2021-04-06 修回日期:2021-05-19 出版日期:2021-07-05 发布日期:2021-06-17
  • 通讯作者: 王婧 E-mail:wangjing@ibms.pumc.edu.cn
  • 基金资助:
    国家重点研发计划;国家自然科学基金

Effects of Colla corii asini on lung function and pathological injury in rats with chronic obstructive pulmonary disease

  • Received:2021-04-06 Revised:2021-05-19 Online:2021-07-05 Published:2021-06-17
  • Contact: Jing WANG E-mail:wangjing@ibms.pumc.edu.cn

摘要: 目的 探究阿胶(CCA)对慢性阻塞性肺疾病(COPD)大鼠肺功能及肺组织病理损伤的影响。方法 将25只大鼠随机分成对照组、模型组、阿胶低、中、高剂量组(1、2、4 g/kg),每组5只。采用卷烟烟雾暴露法造模,烟雾浓度为1100±10 mg/m3,每日1次,每次90 min,连续48周。造模结束次日起,每日灌胃治疗,连续治疗28 d后用动物肺功能仪检测肺功能,HE染色观察肺组织病理。结果 与对照组相比,模型组大鼠潮气量(TV)、每分钟呼气量(EV)、呼气持续时间(Te)、松弛时间(RT)、呼气末期停顿(EEP)和支气管收缩程度(Penh)显著增加(P < 0.05),而呼气峰流速(PEF)、吸气峰流速(PIF)、每分钟通气量(MV)、呼出50%潮气量时呼气流速(EF50)和呼吸频率(f)显著降低(P < 0.05),出现肺气肿和炎性病理性改变,肺组织病变加重。与模型组相比,阿胶高剂量组Te、RT、Penh显著下降(P < 0.05),MV、PIF、EF50显著增加(P < 0.05),肺部炎性细胞浸润减少,肺泡扩张减轻,肺大泡减少,肺气肿程度减轻,肺组织病理损伤明显缓解。结论 阿胶可以改善COPD大鼠肺功能、减轻肺组织炎性反应。

关键词: 阿胶, 慢性阻塞性肺疾病, 肺功能, 肺损伤, 大鼠

Abstract: Objective To explore the effects of Colla Corii Asini (CCA) (common name E’jiao, donkey-hide gelatin) on lung function and pathological injury of the lung tissue in rats with chronic obstructive pulmonary disease (COPD). Methods Twenty-five rats were randomly divided into the control group, COPD model group and treatment groups with low, medium and high-doses of CCA (1, 2, 4 g/kg), with 5 rats in each group. The COPD model group and treatment groups were modeled by cigarette smoke exposure method with a concentration of 1100±10 mg/m3, once a day, 90 minutes each time, for 48 consecutive weeks. After the end of the next day, the CCA groups were given by daily oral gavage treatment for 28 days. Lung function was detected by animal lung function instrument and the pathological changes of the lung tissue were assessed by HE staining. Results Compared with the control group, TV, EV, Te, RT, EEP and Penh were significantly increased in the COPD model group (P < 0.05), while PEF, PIF, MV, EF50 and f were significantly decreased (P < 0.05). Inflammation and pathological changes of emphysema were observed, and lung tissue lesions were aggravated. Compared with the COPD model group, Te, RT and Penh were significantly reduced in the high-dose CCA group (P < 0.05), while MV, PIF and EF50 were significantly higher (P < 0.05). Less infiltration of inflammatory cells, less alveolar expansion and fewer pulmonary bullae were observed in high-dose CCA-treated rats. The degree of emphysema was reduced, and pathological damage to lung tissue was clearly alleviated. Conclusions CCA improve lung function and alleviate the inflammatory response of the lung tissue in COPD rats.

Key words: Colla corii asini, chronic obstructive pulmonary disease, lung function, lung injury, rat

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