羧胺三唑增强小鼠脾脏淋巴细胞促进结肠癌细胞系MC38和C26凋亡

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (6): 805-810.

羧胺三唑增强小鼠脾脏淋巴细胞促进结肠癌细胞系MC38和C26凋亡

高洪婷, 黄雨晴, 杨黎星, 马瑞, 王钰铖, 鞠瑞^*, 郭磊^*

中国医学科学院基础医学研究所北京协和医学院基础学院药理系, 北京 100005

收稿日期:2021-03-09 修回日期:2021-04-18 出版日期:2021-06-05 发布日期:2021-05-31
通讯作者: ^*leiguo@ibms.cams.cn;jurui1984@163.com
基金资助:
国家自然科学基金(81872897);中国医学科学院医学与健康科技创新工程(2016-I2M-1-011)

Carboxyamidotriazole enhances the effects of mouse spleen lymphocytes on promoting the apoptosis of colon cancer cell lines MC38 and C26

GAO Hong-ting, HUANG Yu-qing, YANG Li-xing, MA Rui, WANG Yu-cheng, JU Rui^*, GUO Lei^*

Department of Pharmacology,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005,China

Received:2021-03-09 Revised:2021-04-18 Online:2021-06-05 Published:2021-05-31
Contact: ^*leiguo@ibms.cams.cn;jurui1984@163.com

摘要/Abstract

摘要： 目的探讨羧胺三唑(CAI)直接或通过小鼠脾脏淋巴细胞间接对小鼠结肠癌细胞系(MC38和C26)存活和凋亡的影响。方法体外培养结肠癌细胞系MC38和C26细胞,分离小鼠脾脏淋巴细胞,MC38和C26细胞分别与小鼠脾脏淋巴细胞共培养,不同浓度CAI处理细胞。CCK-8法检测细胞存活,流式细胞测量术检测细胞凋亡以及小鼠脾脏淋巴细胞中CD4⁺CD3⁺ T细胞和CD8⁺CD3⁺ T细胞的比例。结果不同浓度CAI处理MC38和C26细胞后,活细胞数目减少(P＜0.05、P＜0.01和P＜0.001),凋亡率升高(P＜0.01和P＜0.001);MC38和C26细胞分别与小鼠脾脏淋巴细胞共培养48 h后,MC38和C26细胞数目减少(P＜0.001),凋亡率升高(P＜0.001);不同浓度CAI处理小鼠脾脏淋巴细胞共培养条件下的MC38和C26细胞48 h,与单独小鼠脾脏淋巴细胞处理相比,MC38和C26细胞数目进一步减少(P＜0.001),凋亡率进一步升高(P＜0.05和P＜0.001);不同浓度CAI处理小鼠脾脏淋巴细胞48 h后,小鼠脾脏淋巴细胞中CD4⁺CD3⁺ T细胞和CD8⁺CD3⁺ T细胞的比例显著升高(P＜0.05和P＜0.01)。结论 CAI抑制MC38和C26细胞存活、促进细胞凋亡,并可能通过升高小鼠脾脏淋巴细胞中CD4⁺CD3⁺ T细胞和CD8⁺CD3⁺ T细胞的比例增强小鼠脾脏淋巴细胞抑制MC38和C26细胞存活、促进细胞凋亡。

关键词: 羧胺三唑, 小鼠脾脏淋巴细胞, 结肠癌, 凋亡

Abstract: Objective To investigate the direct effects of carboxyamidotriazole(CAI)on the survival and apoptosis of colon cancer cells(MC38 and C26)and its indirect effects through enhancing the anticancer effects of spleen lymphocytes in mice. Methods MC38 and C26 cells were cultured in vitro,spleen lymphocytes of mice were isolated,MC38 and C26 cells were co-cultured with spleen lymphocytes of mice,and the cells were treated with different concentrations of CAI. CCK-8 method was used to detect cell survival,flow cytometry was used to detect cell apoptosis and the proportion of CD4⁺CD3⁺ T cells and CD8⁺CD3⁺ T cells in spleen lymphocytes of mice. Results The number of living cells of MC38 and C26 cells decreased(P＜0.05,P＜0.01 and P＜0.001)and the apoptosis rate was increased(P＜0.01 and P＜0.001)after being treated with different concentrations of CAI. The number of living cells of MC38 and C26 cells was decreased(P＜0.001)upon being co-cultured with spleen lymphocytes of mice for 48 h, and the apoptosis rate was increased(P＜0.001). The number of living cells of MC38 and C26 cells in co- culture groups treated with different concentrations of CAI for 48 h further decreased(P＜0.001)and the apoptosis rate increased(P＜0.05 and P＜0.001)as compared with those co-cultured with spleen lymphocytes of mice without CAI. The proportion of CD4⁺CD3⁺ T cells and CD8⁺CD3⁺ T cells in spleen lymphocytes of mice was significantly increased(P＜0.05 and P＜0.01)after 48 h treatment with different concentrations of CAI. Conclusions CAI can inhibit the survival and promote apoptosis of MC38 and C26 cells,and enhance the effects of spleen lymphocytes in mice on inhibiting the survival and promoting apoptosis of MC38 and C26 cells through a potential mechanism of increasing the proportion of CD4⁺CD3⁺ T cells and CD8⁺CD3⁺ T cells in spleen lymphocytes of mice.

Key words: carboxyamidotriazole, spleen lymphocytes of mice, colon cancer, apoptosis

中图分类号:

高洪婷, 黄雨晴, 杨黎星, 马瑞, 王钰铖, 鞠瑞, 郭磊. 羧胺三唑增强小鼠脾脏淋巴细胞促进结肠癌细胞系MC38和C26凋亡[J]. 基础医学与临床, 2021, 41(6): 805-810.

GAO Hong-ting, HUANG Yu-qing, YANG Li-xing, MA Rui, WANG Yu-cheng, JU Rui, GUO Lei. Carboxyamidotriazole enhances the effects of mouse spleen lymphocytes on promoting the apoptosis of colon cancer cell lines MC38 and C26[J]. Basic & Clinical Medicine, 2021, 41(6): 805-810.

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