基础医学与临床 ›› 2021, Vol. 41 ›› Issue (3): 376-381.

• 研究论文 • 上一篇    下一篇

丹酚酸B减轻LPS诱导的小鼠急性肺损伤

李玉婷1,罗乐2,尹素娟1,朱小兰1   

  1. 1. 永州职业技术学院
    2. 山西医科大学
  • 收稿日期:2020-06-11 修回日期:2020-09-29 出版日期:2021-03-05 发布日期:2021-03-01
  • 通讯作者: 罗乐 E-mail:1243150780@qq.com
  • 基金资助:
    湖南省教育厅科学研究项目

Salvianolic acid B alleviates LPS- induced acute lung injury in mice

  • Received:2020-06-11 Revised:2020-09-29 Online:2021-03-05 Published:2021-03-01

摘要: 目的 研究丹酚酸B减轻脂多糖(LPS)诱导的急性肺损伤。方法 将48只小鼠随机分为对照组、模型组(气管滴注LPS)、丹酚酸B低/中/高剂量干预组、莱菔硫烷阳性对照组,检测肺湿/干重比(W/D)值;检测肺泡灌洗液(BALF)中蛋白浓度;HE染色法评价肺组织病理损伤;ELISA检测BALF中TNF- α、 IL-1 β和 IL-6的含量,检测肺组织MPO、SOD的活性和MDA含量;Western blot检测肺组织中Nrf2、NQO1、HO1、Keap1、NF-κB及p-NF-κB的蛋白表达。结果 与对照组相比,模型组小鼠肺组织产生病理性变化,W/D值、BALF中蛋白质浓度、炎性细胞因子含量明显升高(P<0.05),急性肺损伤模型建立成功。与模型组比较,丹酚酸B干预组的各指标值发生显著变化(P<0.05),减轻LPS诱导的急性肺损伤小鼠氧化应激,且呈浓度依赖性增强,同时降低Keap1的表达(P<0.01),升高Nrf2、NQO1和HO1的表达(P<0.01),抑制LPS诱导的NF-κB的表达和NF-κB的磷酸化。结论 丹酚酸B减轻了LPS诱导的小鼠急性肺损伤。

关键词: 丹酚酸B, 急性肺损伤, 氧化应激, Keap1-Nrf2/ARE信号通路

Abstract: Objective To study whether Salvianolic acid B can reduce LPS induced acute lung injury .Methods 48 mice were randomly divided into control group, model group (intratracheal instillation of LPS), Salvianolic acid B low / medium / high dose intervention group, and sulforaphane positive control group. The lung wet / dry weight ratio (W/D) was detected; the protein concentration of alveolar lavage fluid (BALF) was detected; lung tissue pathological damage was evaluated by HE staining; TNF-α,IL-1βand IL-6 in BALF were detected by ELISA .Western blot was used to detect the protein expression of Nrf2, NQO1, HO1, Keap1,NF-κB and p -NF-κB in lung tissue. Results compared with the control group, pathological changes were observed in the lung tissue of the model group,W/D , protein concentration in BALF and inflammatory cytokine content were significantly increased (P < 0.05). Compared with the model group, Salvianolic acid B intervention group had significant changes (P < 0.05), alleviated the oxidative stress induced by LPS, and increased the expression of Keap1 (P < 0.01), increased the expression of Nrf2, NQO1 and HO1 (P < 0.01), inhibited the activation of NF-κB and p-NF-κB induced by LPS. Conclusion Salvianolic acid B can alleviate LPS induced acute lung injury in mice.

Key words: Salvianolic acid B, acute lung injury, oxidative stress, Keap1-Nrf2 /ARE signaling pathway

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