miR-205-5p抑制体外胃癌细胞系增殖、迁移和侵袭

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (8): 1068-1075.

miR-205-5p抑制体外胃癌细胞系增殖、迁移和侵袭

杨屹立, 何学元, 潘新民, 马建勋^*

甘肃省人民医院普外科, 甘肃兰州 730000

收稿日期:2019-06-28 修回日期:2019-11-12 出版日期:2020-08-05 发布日期:2020-07-29
通讯作者: ^*majianxun68@sina.com
基金资助:
甘肃省科技计划(18JR3RA330);甘肃省人民医院院内科研基金(18GSSY4-44)

miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines

YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun^*

Department of General Surgery, Gansu Provincial People's Hospital, Lanzhou 730000, China

Received:2019-06-28 Revised:2019-11-12 Online:2020-08-05 Published:2020-07-29
Contact: ^*majianxun68@sina.com

摘要/Abstract

摘要： 目的探讨miR-205-5p靶向RAS致癌家族基因2B(RAP2B)对胃癌细胞增殖、迁移和侵袭的影响。方法 RT-qPCR和Western blot检测胃癌细胞AGS、MGC803、MKN-28、SGC-7901和正常胃黏膜细胞GES-1中miR-205-5p和RAS致癌家族基因2B的表达。分别构建过表达miR-205-5p和抑制RAP2B表达的AGS细胞株,采用MTT法检测细胞活力;Transwell小室法检测细胞的迁移及侵袭能力;Western blot检测RAP2B、cyclin D1、MMP-2、MMP-9、GSK-3β和β-catenin蛋白的表达。采用双荧光素酶报告基因实验验证miR-205-5p对RAP2B的靶向作用。结果与正常胃黏膜细胞相比,4种胃癌细胞中miR-205-5p的表达显著降低,RAP2B的表达显著升高(P＜0.05)。过表达miR-205-5p或抑制RAP2B表达均可显著抑制AGS细胞的增殖、迁移和侵袭,抑制cyclin D1、MMP-2和MMP-9蛋白的表达(P＜0.05)。miR-205-5p可负性调控RAP2B的表达。结论 miR-205-5p通过靶向RAP2B抑制胃癌细胞的增殖、迁移和侵袭。

关键词: miR-205-5p, RAP2B, 胃癌, 增殖, 迁移

Abstract: Objective To investigate the effect of miR-205-5p on the proliferation, migration and invasion of gastric cancer cells by targeting at RAS oncogene family (RAP2B). Methods RT-qPCR and Western blot were used to detect the expression of miR-205-5p and RAP2B in gastric cancer cell lines AGS, MGC803, MKN-28, SGC-7901 and normal gastric mucosal cell line GES-1. Over expressing miR-205-5p, inhibition of RAP2B, cell viability was detected by MTT assay; cell migration and invasion ability were detected by Transwell method; The expression of RAP2B, cyclin D1, MMP-2, MMP-9 GSK-3β and β-catenin proteins were detected by Western blot. The dual luciferase reporter gene assay was used to verify the targeting of miR-205-5p to RAP2B. Results Compared with normal gastric mucosal cells, the expression of miR-205-5p was significantly decreased in four gastric cancer cell lines, and the expression of RAP2B was significantly increased (P＜0.05). The over-expression of miR-205-5p or inhibition of RAP2B inhibited the proliferation, migration and invasion of AGS cells and inhibited the expression of cyclin D1, MMP-2 and MMP-9 proteins (P＜0.05). miR-205-5pnegatively regulated the expression of RAP2B. Conclusions miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cells in vitro by targeting at RAP2B.

Key words: miR-205-5p, RAP2B, gastric cancer, proliferation, migration

中图分类号:

R735.2

杨屹立, 何学元, 潘新民, 马建勋. miR-205-5p抑制体外胃癌细胞系增殖、迁移和侵袭[J]. 基础医学与临床, 2020, 40(8): 1068-1075.

YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun. miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines[J]. Basic & Clinical Medicine, 2020, 40(8): 1068-1075.

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