miR-195-5p表达上调抑制肺癌细胞系A549的迁移和侵袭

›› 2019, Vol. 39 ›› Issue (12): 1694-1698.

miR-195-5p表达上调抑制肺癌细胞系A549的迁移和侵袭

张杨,杜雯雯,刘婷,曾园园,黄建安

苏州大学附属第一医院

收稿日期:2019-02-14 修回日期:2019-04-24 出版日期:2019-12-05 发布日期:2019-12-04
通讯作者: 黄建安 E-mail:huang_jian_an@163.com
基金资助:
江苏省重点医学学科与临床重点专业;苏州呼吸内科重点实验室;苏州临床医学中心;江苏省研究生科研与实践创新计划项目

Up-regulation expression of miR-195-5p inhibits the migration and invasion of lung cancer cell line A549

Received:2019-02-14 Revised:2019-04-24 Online:2019-12-05 Published:2019-12-04
Supported by:
the Jiangsu Provincial Key Medical Discipline and the Clinical Key Specialty Project of China;the Suzhou Key Laboratory for Respiratory Medicine;the Clinical Medical Center of Suzhou;Postgraduate Research & Practice Innovation Program of Jiangsu Province

摘要/Abstract

摘要： 目的探讨miR-195-5p对肺癌细胞系A549细胞迁移和侵袭的影响及其机制。方法实时荧光定量PCR检测肺癌组织及肺癌细胞系中miR-195-5p的表达；通过脂质体介导将miR-195-5p模拟物作用于A549细胞，用Transwell法检测细胞迁移和侵袭；Western blot检测细胞内上皮间质转化标志物及ZEB1的表达。结果相对于癌旁组织及人正常肺上皮细胞，miR-195-5p在肺癌组织及肺癌细胞系中低表达（P <0.001）；过表达miR-195-5p可显著抑制A549细胞的迁移和侵袭（P <0.001），伴随E-cadherin蛋白表达的上调，N-cadherin和vimentin蛋白表达的下调（P <0.01）；过表达miR-195-5p可抑制A549细胞ZEB1的表达（P <0.001）。结论 miR-195-5p可能通过下调ZEB1的表达，抑制肺癌细胞迁移和侵袭。

关键词: miR-195-5p, ZEB1, 肺癌, 迁移, 侵袭

Abstract: Objective To investigate the effect and mechanism of miR-195-5p on the migration and invasion of A549 cells in vitro. Methods The expression of miR-195-5p in lung cancer tissues, paracancerous tissues and lung cancer cell lines were analyzed by quantitative real-time PCR. Moreover, miR-195-5p mimics were transfected into A549 cells by liposome-mediated method. The cell migration and invasion were examined by Transwell assay. Western blot analysis was used to detect the effect of miR-195-5p on the protein level expression of epithelial-mesenchymal transition makers and ZEB1. Results Compared with paracancerous tissues and normal lung epithelial cells, the expressions of miR-195-5p were lower in lung cancer tissues and lung cancer cell line（P <0.001）. The over-expression of miR-195-5p inhibited the migration and invasion of A549 cells（P <0.001）, increased the protein expression of E-cadherin and reduced expression of N-cadherin、vimentin and ZEB1（P <0.01）. Conclusions miR-195-5p inhibits the migration and invasion of lung cancer cells by reducing the expression of ZEB1.

Key words: miR-195-5p, ZEB1, lung cancer, migration, invasion

中图分类号:

R734.2

张杨杜雯雯刘婷曾园园黄建安. miR-195-5p表达上调抑制肺癌细胞系A549的迁移和侵袭[J]. , 2019, 39(12): 1694-1698.

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