关键词: miR-195, SUZ12, 舌鳞癌, 增殖, 迁移, 侵袭

Abstract: Objective To study the effect of miR-195 on cell proliferation, migration and invasion of CAL27 cells, and explore its mechanism. Methods RT-qPCR was used to detect the expression of miR-195 in tongue squamous cell carcinoma and adjacent normal tissues and cells. The miR-195 group (transfected miR-195 mimics), miR-con group (transfected miR-NC), si-con group (transfected si-con), si-SUZ12 group (transfected si-SUZ12), miR-195+Ctrl group (co-transfected miR-195 mimics and pcDNA 3.1), miR-195+ SUZ12 group (co-transfected miR-195 mimics and pcDNA 3.1-SUZ12); MTT assay was used to detect the proliferation of each group; Transwell was used to detect the migration and invasion of each group; Western blot was used to detect the protein expression of SUZ12 in each group; dual fluorescein report gene detection assay was used to detect the fluorescence activity of each group cells. Results Compared with normal tissues adjacent to the cancer, the expression of miR-195 was significantly decreased in tongue squamous carcinoma. Compared with human normal oral keratinocytes NHOK, the expression of miR-195 was significantly decreased in tongue squamous carcinoma cells (P<0.05). Overexpression miR-195 and knockdown SUZ12 significantly inhibited the proliferation, migration and invasion of CAL27 cells; SUZ12 is the target of miR-195. Overexpression of SUZ12 rescued the inhibitory effect of miR-195 on proliferation, migration and invasion of CAL27 cells. Conclusions miR-195 can inhibit the proliferation, migration and invasion of CAL27 cell. The mechanism may be related to targeting SUZ12, which will provide a new target for the prevention and treatment of tongue squamous cell carcinoma.

Key words: miR-195, SUZ12, TSCC, proliferation, migration, invasion