孟鲁司特抑制哮喘模型大鼠气道黏液高分泌

›› 2019, Vol. 39 ›› Issue (12): 1699-1703.

孟鲁司特抑制哮喘模型大鼠气道黏液高分泌

张博达¹,李俊玲²,金典²,陈思敏²,董艳²

1. 川北医学院附属医院
2. 成都中医药大学附属医院

收稿日期:2018-11-27 修回日期:2019-03-27 出版日期:2019-12-05 发布日期:2019-12-04
通讯作者: 张博达 E-mail:279758645@qq.com
基金资助:
三子养亲汤通过抑制 CysLTs 通路改善哮喘症状的机制研究

Montelukast inhibits hypersecretion of airway mucus in asthmatic rats

Received:2018-11-27 Revised:2019-03-27 Online:2019-12-05 Published:2019-12-04

摘要/Abstract

摘要： 目的探讨白三烯(LTs)介导的炎性损伤对哮喘大鼠气道黏液高分泌的作用。方法用卵蛋白（OVA）经典造模方法复制哮喘大鼠模型，用白三烯特异性受体拮抗剂孟鲁司特行干预性治疗，并使用ELISA、Western blot和RT-qPCR等方法观察炎性反应及气道黏液分泌指标变化。结果孟鲁司特可抑制CysLTs受体1(P<0.05)，抑制P38MAPK磷酸化(P<0.05)，下调MUC5AC基因表达及蛋白合成(P<0.05)。结论白三烯介导的炎性反应在哮喘大鼠模型中有促进气道黏液高分泌的作用，这一作用能被孟鲁司特所抑制。

关键词: 孟鲁司特哮喘气道黏液高分泌

Abstract: Objective To investigate the effect of leukotriene-mediated inflammatory injury on hypersecretion of airway mucus in asthmatic rats.Methods The rat model of asthma was duplicated with OVA classic modeling method, and the CysLTs1 specific receptor antagonist montelukast was used for ELISA、Western blot and q-PCR to observe the changes of inflammation and airway mucus secretion indexes.Results Montelukast could inhibit CysLTs receptor 1,P38MAPK phosphorylation, and down-regulate MUC5AC gene expression and protein synthesis. Conclusions Leukotriene-mediated inflammation is shown to promote airway mucus hypersecretion in asthmatic rats, which could be antagonized by montelukase.

Key words: montelukast asthma hypersecretion of airway mucus

张博达李俊玲金典陈思敏董艳. 孟鲁司特抑制哮喘模型大鼠气道黏液高分泌[J]. , 2019, 39(12): 1699-1703.