›› 2019, Vol. 39 ›› Issue (11): 1543-1547.

• 研究论文 • 上一篇    下一篇



  1. 1. 济宁医学院
    2. 山东大学
  • 收稿日期:2019-02-14 修回日期:2019-04-24 出版日期:2019-11-05 发布日期:2019-11-05
  • 通讯作者: 王春梅 E-mail:wangchunmei410@163.com
  • 基金资助:

Soybean isoflavone reduces cerebral ischemia-reperfusion injury in rats

  • Received:2019-02-14 Revised:2019-04-24 Online:2019-11-05 Published:2019-11-05

摘要: 目的 研究大豆异黄酮(SI)对大鼠脑缺血-再灌注(I/R)损伤保护作用的分子机制。方法 将大鼠随机分为假手术组(sham)、模型组(I/R)制作脑缺血模型、大豆异黄酮(SI)干预组(50、100和200 mg/kg)。2,3,5-氯化三苯基四氮唑(TTC)染色法检测脑梗死体积;HE染色检测脑组织病理变化;ELISA测定脑组织中超氧化物歧化酶SOD活性,IL-6、TNF-α、cAMP含量;Western blot检测皮层p-ERK1/2的表达。结果 与sham组相比,I/R组出现明显的梗死灶(P<0.05),SI干预组(100和200 mg/kg),脑梗死灶体积明显降低(P<0.05)。模型组细胞间隙增宽,细胞核固缩,SI干预组神经细胞的病理变化有所改善。与sham组相比,I/R组脑组织中SOD活性和cAMP含量显著降低,SI干预组(100和200 mg/kg)脑组织中SOD活性和cAMP含量升高(P<0.05);与sham组相比,I/R组IL-6和TNF-α含量均显著升高,SI干预组(100和200 mg/kg)IL-6和TNF-α的含量明显降低(P<0.05)。与sham相比,I/R组中p-ERK1/2表达升高,SI干预组(100和200 mg/kg)p-ERK1/2表达降低(P<0.01)。结论 SI能够有效改善大鼠脑I/R引起的氧化应激及炎性反应,提示SI对大鼠脑I/R的保护作用机制可能与SI抗氧化及炎性反应有关。

关键词: 脑缺血-再灌注, 大豆异黄酮, 氧化应激, 炎性反应

Abstract: Objective To study the molecular mechanism of protective effects of soybean isoflavones (SI) on cerebral ischemia-reperfusion (I/R) injury in rats. Methods Rats were randomly divided into sham operation group (sham), ischemia-reperfusion group (I/R), soybean isoflavones (SI) intervention group(50, 100 and 200 mg/kg) . 2,3,5-triphenylte-trazolium chloride (TTC) staining method was used to detect the cerebral infarction volume. HE staining was used to detect the pathological changes of brain tissues. The activity of superoxide dismutase SOD and the contents of IL-6, TNF-α, cAMP were measured by ELISA. Western blot was used to detect the level of p-ERK1/2. Results The I/R group had obvious infarct foci compared with the sham group (p<0.05), but the cerebral infarct volume significantly decreased after SI intervention(100 and 200 mg/kg) (p<0.05), respectively. In model group, cell gap was widened and nucleus became pyknosis. After the intervention of SI group, the pathological changes of cells were improved. Compared with the sham group, the activity of SOD and the content of cAMP in I/R group were significantly reduced, but after SI intervention (100 and 200 mg/kg), the activity of SOD and the content of cAMP were increased ( p<0.05). Compared with the sham group, the content of IL-6 and TNF-α in I/R group increased, but they were decreased after intervented with SI (100 and 200 mg/kg) ( p<0.05). Compared with the sham group, the expression of p-ERK1/2 in the model group was increased, and the expression of p-ERK1/2 was decreased after SI intervention (100 and 200 mg/kg) (p< 0.01). Conclusion SI can effectively improve oxidative stress and inflammatory response induced by I/R in rats. It is suggested that the protective mechanism of SI on cerebral I/R in rats may be related to the antioxidant and inflammatory reaction of SI.

Key words: cerebral ischemia-reperfusion injury, soybean isoflavones, oxidative stress, inflammatory response