TRIM11靶向调控miR-24-3p促进乳腺癌MCF7细胞系增殖和侵袭

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (4): 451-457.

TRIM11靶向调控miR-24-3p促进乳腺癌MCF7细胞系增殖和侵袭

张洋¹,张静²,季琳¹,李文媛¹,王莹¹,孙平¹,于伟光³

1. 牡丹江医学院
2. 牡丹江医学院解剖教研室
3. 牡丹江医学院红旗医院

收稿日期:2017-03-30 修回日期:2017-05-30 出版日期:2018-04-05 发布日期:2018-03-27
通讯作者: 张洋 E-mail:978619420@qq.com
基金资助:
国家自然科学基金;黑龙江省自然科学基金项目

TRIM11 promotes proliferation and invasion of breast cancer cell line MCF7 by targeting miR-24-3p

Received:2017-03-30 Revised:2017-05-30 Online:2018-04-05 Published:2018-03-27

摘要/Abstract

摘要： 目的探讨TRIM11 靶向调控microRNA-24-3p（miR-24-3p）对乳腺癌细胞增殖和侵袭的影响，明确TRIM11高表达与乳腺癌预后的相关性。方法免疫组化法检测TRIM11在31例乳腺癌和31例癌旁正常组织中的表达。用siRNA TRIM11和miR-24-3p慢病毒转染人乳腺癌MCF7细胞，实时荧光定量PCR（RT-qPCR）和Western bolt检测TRIM11及miR-24-3p mRNA及蛋白表达变化及相关性分析；荧光素酶报告实验验证miR-24-3p为TRIM11的潜在靶点；MTT法和Transwell检测细胞增殖数量、增殖活力和侵袭。结果 TRIM11在乳腺癌组织及MCF7细胞中表达显著增高(P＜0.05)。TRIM11高表达患者生存率显著降低(P＜0.05)。siRNA TRIM11转染显著抑制MCF7细胞中TRIM11表达，而且抑制MCF7细胞增殖数量、增殖活力和侵袭能力(P＜0.05)。miR-24-3p显著降低野生型3，-UTR TRIM11荧光素酶活性(P＜0.05)，但突变型没有作用。miR-24-3p mRNA在MCF7细胞下调，miR-24-3p抑制MCF7细胞TRIM11蛋白和mRNA 表达，miR-24-3p mRNA与TRIM11 mRNA在MCF7细胞中表达显著负相关(P＜0.05)，miR-24-3p抑制MCF7细胞增殖数量、增殖活力和侵袭能力(P＜0.05)。结论 TRIM11在乳腺癌组织及MCF7细胞中表达上调，并可能通过靶向调控miR-24-3p促进乳腺癌细胞增殖和侵袭，进而影响乳腺癌预后，TRIM11/ miR-24-3p轴有望成为治疗乳腺癌的新靶点。

关键词: TRIM11, miR-24-3p, 乳腺癌, 增殖, 侵袭

Abstract: Objective To investigate the effect of TRIM11 (tripartite motif-containing protein 11) target regulating MicroRNA-24-3p (miR-24-3p) on the proliferation and invasion of breast cancer cells, and the relation between TRIM11 high expression and the prognosis of breast cancer. Methods Immunohistochemical method was used to detect the expression of TRIM11 in 31 cases of breast cancer and 31 cases of adjacent breast cancer normal tissues. The siRNA TRIM11 lentivirus and miR-24-3p lentivirus was transfected into human breast cancer MCF7 cell lines, observe the correlation expression of TRIM11 and miR-24-3p mRNA and protein by using real-time fluorescence quantitative PCR (RT-qPCR) and Western bolt, luciferase reporter experiments was used to verify that the miR-24-3p as a direct target of TRIM11, MTT and Transwell were used to investigate the cells proliferation, activity and invasion. Results The expression of TRIM11 in breast cancer tissues or MCF7 cells was significantly higher (P < 0.05), and we found TRIM11 high expression predicts poor prognosis in breast cancer patients (P < 0.05). siRNA TRIM11 significantly inhibited the expression of TRIM11 in MCF7 cells, moreover, which inhibited the MCF7 cells proliferation, viability and invasion (P < 0.05). miR-24-3p significantly reduced 3，-UTR TRIM11 luciferase activity in wild-type (P < 0.05), however, no effect was found in mutant type. The expression of miR-24-3p was decreased, miR-24-3p and TRIM11 mRNA expression was negatively correlated in MCF7 cells (P < 0.05), miR-24-3p inhibition protein and mRNA expression of TRIM11 in MCF7 cells, and inhibit MCF7 cells proliferation, viability and invasion (P<0.05). Conclusions The expression of TRIM11 is up-regulated in breast cancer and cells, promote the proliferation and invasion of breast cancer cells though regulating miR-24-3p expression, TRIM11 high expression predicts poor prognosis in breast cancer, TRIM11/ miR-24-3p axis is expected to become a new target for treatment of breast cancer.

Key words: TRIM11, miR-24-3p, breast cancer, proliferation, invasion

中图分类号:

张洋张静季琳李文媛王莹孙平于伟光. TRIM11靶向调控miR-24-3p促进乳腺癌MCF7细胞系增殖和侵袭[J]. 基础医学与临床, 2018, 38(4): 451-457.

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