关键词: miR-548p, NF-κB, 卵巢癌细胞, 增殖, 迁移

Abstract: Objective To study the effects of miR-548p on the proliferation and migration of SKOV-3 cells in ovarian cancer cell lines in vitro, and its relevant mechanism. Methods The expression of miR-548p in ovarian cancer tissues,paracancerous tissue and ovarian cancer cell lines were analyzed by Real-time fluorescence quantitative PCR. Moreover, the effects of miR-548p on SKOV-3 cell proliferation and migration were explored by MTT and Transwell assays using miR-548p mimics in SKOV-3 cells. Western blot method was used to detect the effects of miR-548p on matrix metalloproteinases, mitochondrial apoptosis related proteins and NF-κB signaling pathway in SKOV-3 cells. Results MiR-548p was poorly expressed in ovarian tissues and ovarian cancer cell lines compared to adjacent normal ovary tissues and cell lines. The increase of miR-548p expression inhibited the proliferation and migration of SKOV-3 cells, decreased the related MMP-2, MMP-9 and Bcl-2 expression, and increased Bax expression. Conclusion MiR-548p regulates the proliferation and migration of ovarian cancer cells through reducing the phosphorylation level of p65, and provides a new target for the treatment of ovarian cancer.

Key words: miR-548p, NF-κB signaling pathways, Ovarian cancer cells, Proliferation, Migration