MST1过表达抑制宫颈癌SiHa细胞系的增殖、迁移和侵袭

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (3): 351-354.

MST1过表达抑制宫颈癌SiHa细胞系的增殖、迁移和侵袭

郑小影¹,郝云涛²,马卫军¹,韩莉¹,赵淑敏³,连相尧²,张玉娟²

1. 承德医学院电镜室
2. 承德医学院附属医院
3. 承德医学院

收稿日期:2016-09-01 修回日期:2016-11-01 出版日期:2017-03-05 发布日期:2017-02-23
通讯作者: 赵淑敏 E-mail:zhaoshumin-2008@163.com
基金资助:
河北省卫计委医学科学研究重点课题计划项目;承德市科技局科学研究与发展计划项目

Overexpression of MST1 inhibits the proliferation, migration and invasion of cervical cancer cell line SiHa

Received:2016-09-01 Revised:2016-11-01 Online:2017-03-05 Published:2017-02-23

摘要/Abstract

摘要： 目的探讨哺乳动物不育系20样激酶1(MST1)对子宫颈癌SiHa细胞增殖、迁移和侵袭能力的影响。方法 Western blot检测正常宫颈上皮细胞 H8与宫颈癌 SiHa 细胞中 MST1 的表达；构建pJ3H-HA-MST1质粒并转染SiHa细胞系，Western blot检测MST1、HA、Ki-67和MMP9的蛋白表达；MTS、划痕实验和Transwell分别检测细胞增殖、迁移和侵袭能力。结果 SiHa细胞的MST1蛋白表达水平明显低于H8细胞（P<0.05）；SiHa细胞转染MST1质粒后，MST1蛋白表达明显升高，而Ki-67和MMP9蛋白表达水平显著下降（P<0.05），SiHa细胞的增殖被明显抑制（P<0.05），同时细胞的迁移和侵袭能力也显著降低（P<0.01）。结论 MST1过表达可以抑制宫颈癌SiHa细胞的增殖、迁移和侵袭能力。

关键词: 哺乳动物不育系20样激酶1, 宫颈癌, 增殖, 迁移, 侵袭

Abstract: Objective To discuss the influence of MST1 (mammalian sterile 20-like kinase 1，MST1) on the proliferation，migration and invasion of SiHa cervical cancer. Methods Western blot was used to detect the expression of MST1 in cervical epithelial cells H8 and cervical cancer cells SiHa; PJ3H-HA-MST1 was constructed and transfected it to SiHa cell by LipofectamineＴＭ3000; MST1、 HA、Ki-67 and MMP9 protein expression were evaluated by Western blot; While the proliferation，migration and invasion of SiHa cell were assessed by MTS、scratch adhesion test and Transwell assay respectively. Results Compared SiHa cells with H8 cells，MST1 expression in SiHa cells was significantly lower than that of H8 cells．The plasmid was successfully transfected into SiHa cells, MST1 expression was significantly higher, while the expression of Ki-67 and MMP9 were sharply lower, and the proliferation、migration and invasion ability were all suppressed significantly. Conclusions Overexpression of MST1 can inhibit the proliferation、migration and invasion of cervical cancer cell line SiHa.

Key words: mammalian sterile 20-like kinase 1, cervical cancer, proliferation, migration, invasion

郑小影郝云涛马卫军韩莉赵淑敏连相尧张玉娟. MST1过表达抑制宫颈癌SiHa细胞系的增殖、迁移和侵袭[J]. 基础医学与临床, 2017, 37(3): 351-354.

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