基础医学与临床 ›› 2014, Vol. 34 ›› Issue (2): 168-171.

• 研究论文 • 上一篇    下一篇

阿托伐他汀下调大鼠心梗后线粒体融合素2表达和细胞凋亡

周炜,陈玲,周逸,陈曼华   

  1. 武汉市中心医院
  • 收稿日期:2013-08-14 修回日期:2013-09-25 出版日期:2014-02-05 发布日期:2014-01-13
  • 通讯作者: 陈曼华 E-mail:chenmh@aliyun.com
  • 基金资助:
    武汉市卫生局临床医学科研项目;武汉市科技局关键技术攻关计划

Atorvastatin down regulating mitofusin 2 gene expression and cell apoptosis

  • Received:2013-08-14 Revised:2013-09-25 Online:2014-02-05 Published:2014-01-13

摘要: 目的 研究阿托伐他汀通过下调线粒体融合素2表达抑制大鼠心肌梗死后细胞凋亡。 方法 雄性SD大鼠48只,随机分为假手术组(Sham),心肌梗死组(MI),阿托伐他汀1组(Statin 1)和2组(Statin 2),每组12只。分别于术前7天开始每日灌胃,给予阿托伐他汀10和40mg/kg,MI组以蒸馏水灌胃,随后结扎大鼠前降支建立心肌梗死模型。术后24h用TUNEL法检测心肌细胞凋亡,免疫组化法检测线粒体融合素2表达,免疫印迹法检测磷酸化蛋白激酶B(p-Akt)表达。 结果 与Sham组相比,MI组心肌细胞凋亡显著增加,线粒体融合素2表达显著增加,p-Akt表达显著下降(p<0.01);与MI组相比,Statin 1和2组心肌细胞凋亡和线粒体融合素2表达均显著降低(p<0.05),而p-Akt表达显著增高(p<0.05),且Statin 2组较1组变化更显著(p<0.05)。结论 阿托伐他汀可抑制大鼠心肌梗死后的细胞凋亡,其作用可能与下调线粒体融合素2表达有关。

关键词: 阿托伐他汀, 心肌梗死, 凋亡, 线粒体融合素2基因

Abstract: Objective To investigate the effects of atorvastatin on myocardial apoptosis and mitofusin 2(Mfn2) expression after acute myocardial infartion(MI) in rats. Methods A total of 48 male Sprague-Dawley rats were randomly divided into sham-operated group, myocardial infartion(MI) group, statin group 1 and group 2. The rats in the two statin groups were given atorvastatin 10 mg?kg-1 or 40 mg?kg-1 daily, respectively. The rats in the MI and statin groups were induced acute myocardial infartion. 24 hours after operation, the MI areas of rat hearts were harvested, and myocardial apoptosis was assessed by in situ terminal deoxynucleotidyl transferase(TdT)-dUTP nick-end labeling(TUNEL staining). Immunohistochemistry staining and western blotting were performed to measure the expression of Mfn 2 and phosphorylated Akt(p-Akt) . Results On the 24 hours after operation, compared with those of the sham group, the myocardial apoptosis and Mfn2 protein expression of the MI groups remarkably increased(p<0.01). Compared with those of the MI group, myocardial apoptosis and Mfn2 protein expression of statin groups significantly decreased with a slightly dose-dependent manner(p<0.05). Conclusion Atorvastatin attenuates myocardium apoptosis after acute myocardial infarction in rats, which may relate to down-regulated expression of mitofusin 2.

Key words: Atorvastatin, Myocardial Infarction, Apoptosis, Mitofusin 2

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