干扰素α通过P204和P21诱导大鼠原代血管平滑肌细胞凋亡

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (12): 1635-1639.

干扰素α通过P204和P21诱导大鼠原代血管平滑肌细胞凋亡

龙向淑¹,吴强²,宋方²,黄晶³,张林军¹

1. 贵州省人民医院
2. 贵州省心血管病研究所，贵州省人民医院
3. 贵州省人民医院心血管内科

收稿日期:2014-04-08 修回日期:2014-06-19 出版日期:2014-12-05 发布日期:2014-11-25
通讯作者: 吴强 E-mail:waqqaa@yahoo.com.cn
基金资助:
贵州省优秀科技教育人才省长专项资金;国家自然科学基金项目

Interferon alpha induces apoptosis of rat primary vascular smooth muscle cells via P204 and P21

Received:2014-04-08 Revised:2014-06-19 Online:2014-12-05 Published:2014-11-25

摘要/Abstract

摘要： 目的探讨干扰素-α（IFN-α）对大鼠血管平滑肌细胞（VSMCs）凋亡的影响。方法应用转染IFI204 siRNA和/或IFN-α瞬时干预体外培养的大鼠VSMCs，以非特异性siRNA转染组为对照组，RT-PCR法检测P204及P21 mRNA表达，Western blot检测P204及P21蛋白表达，流式细胞仪Annexin-V FITC/PI法检测细胞凋亡。结果与对照组比较，IFN-α组P204 mRNA和蛋白表达上调（P＜0.01），细胞凋亡增多（P＜0.01），伴P21 mRNA和蛋白表达增多（P＜0.01）；IFI204 siRNA转染组P204 mRNA和蛋白表达下调（P＜0.01），细胞凋亡减少（P＜0.01），P21 mRNA和蛋白表达减少（P＜0.01）；转染IFI204 siRNA后再加IFN-α干预，P204 mRNA及蛋白表达下调（P＜0.01），但P21mRNA及蛋白表达增多（P＜0.01），促进细胞凋亡（P＜0.01）。结论 P204及P21参与IFN-α诱导大鼠VSMCs凋亡过程的调控。

关键词: 干扰素, 血管平滑肌细胞, 凋亡, 干扰素诱导蛋白, P204

Abstract: Objective To study the effect of interferon alpha (IFN-α) on vascular smooth muscle cells (VSMCs) apoptosis in rats. Methods Cultured VSMCs were treated with transfection IFI204 siRNA and/or IFN-αin vitro instantaneously. Nonspecific siRNA transfection group was as control group. The expression of P204 and P21 mRNA was determined by semiquantitative RT-PCR. P204 and P21 protein was analyzed by Western blotting. The cell apoptosis was analyzed by flow cytometry with Annexin-V FITC/PI method. Results Compared with control group, in IFN-αinduction group, the expression of P204 mRNA and protein was up-regulated(P＜0.01), the cell apoptosis was increased (P＜0.01), and the expression of P21 mRNA and protein was up-regulated (P＜0.01). In IFI204 transfection group, the expression of P204 mRNA and protein was down-regulated(P＜0.01), the cell apoptosis was decreased (P＜0.01), and the expression of P21 mRNA and protein was down-regulated (P＜0.01). When intervention with IFN-α after transfection IFI204 siRNA, the expression of P204 mRNA and protein was down-regulated (P＜0.01), but the expression of P21 mRNA and protein were not decreased correspondingly(P＜0.01), and cell apoptosis was increased (P＜0.01), Conclusion P204 and P21 participation in interferon-α regulation of cell apoptosis of vascular smooth muscle cells in rats.

Key words: interferon, vascular smooth muscle cells, apoptosis, interferon-inducible protein, P204

中图分类号:

R363

龙向淑吴强宋方黄晶张林军. 干扰素α通过P204和P21诱导大鼠原代血管平滑肌细胞凋亡[J]. 基础医学与临床, 2014, 34(12): 1635-1639.

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