基础医学与临床 ›› 2014, Vol. 34 ›› Issue (1): 62-67.

• 研究论文 • 上一篇    下一篇

JAK2/STAT3通路参与大鼠坐骨神经结扎神经病理性疼痛模型

薛照静1,申乐2,王之遥1,黄宇光3   

  1. 1. 中国医学科学院北京协和医学院北京协和医院
    2. 中国医学科学院北京协和医院麻醉科
    3. 中国医学科学院 北京协和医学院 北京协和医院 麻醉科
  • 收稿日期:2013-09-26 修回日期:2013-11-15 出版日期:2014-01-05 发布日期:2013-12-26
  • 通讯作者: 黄宇光 E-mail:pumchhyg@yahoo.com.cn
  • 基金资助:
    神经病理性疼痛相关微小RNA在中枢感觉神经传导通路中的功能研究;miR-203活化的JAK-STAT信号通路传导对神经病理性疼痛机制及干预的研究

JAK2/STAT3 pathway is involved in bilateral chronic constriction injury rat neuropathic pain model

  • Received:2013-09-26 Revised:2013-11-15 Online:2014-01-05 Published:2013-12-26
  • Contact: HUANG Yu-guang E-mail:pumchhyg@yahoo.com.cn

摘要: 目的 结扎大鼠双侧坐骨神经复制bCCI模型,观察JAK2/STAT3通路的激活情况。 方法 60只质量180~200g SD雌性大鼠随机分为3组, bCCI组,Sham组及Naive组。分别于bCCI手术术前1d,术后3、7、14和21d取腰段脊髓背角,并进行RT-PCR及Western blot观察通路中主要因子的mRNA 水平及蛋白水平的变化。 结果 bCCI组大鼠对机械、热刺激及冷丙酮刺激的痛觉阈值明显降低。与Sham组及Na?ve相比,bCCI组STAT3、SOCS3、JAK2及IL-6mRNA水平显著升高,且3组在不同时间点具有差异(P<0.05)。与Sham组及Na?ve相比,bCCI组P-STAT3、JAK2和SOCS3蛋白水平升高。结论 大鼠bCCI神经病理性疼痛模型JAK2/STAT3通路激活。

关键词: 关键词:JAK2, STAT3, 神经病理性疼痛

Abstract: Objective: To evaluate the contribution of the JAK2/STAT3 pathway to neuropathic pain,we examined the effects of this pathway in a rat model of bilateral chronic constriction injury (bCCI). Methods: A rat model of bCCI was established and 60 rats’ behavior tests were performed on the day before surgery and on day 3、7、14 and 21 after surgery, L4~L6 dorsal spinal cord was harvested at the each time point. RT-PCR and Western blot were performed to explore the activation of JAK2/STAT3 pathway. Results: Pain-related behavioral tests socres in the bCCI rats were significant decreased as compared to the sham-operated and na?ve group at each time point postoperatively (P<0.05). SOCS3 mRNA and STAT3 mRNA significantly increased on day 14, accompanied by JAK2mRNA of with a similar time course. IL-6 mRNA level increased on day 3 and showed statistically significant increases on day 21. Western blot analysis showed that JAK2, P-STAT3, SOCS3 increased at different timepoints. Conclusion:Our results suggest that the JAK2/STAT3 pathway in the spinal dorsal horn was significantly upregulated in a rat bCCI model of neuropathic pain which will open new avenues for therapeutic intervention.

Key words: Key words: JAK2, STAT3, neuropathic pain

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