Abstract: Objective To discuss the effects of nandrolone styrene acrylic acid on islet cells under the condition of oxidative stress for the theoretical basis of the treatment of type 2 diabetes. Methods NIT-1 cell were divided into four groups which in different concentrations of glucose (5.6,11.1,16.7 and 27.6 mmol/L),then treat with 10μg/mL Benzyl propionate nandrolone for 48h. Nampt and FOXO1 protein expression were detected by Western blot assay. Cell cycle was determined by FCM and cell insulin secreted level was measured with radioimmuno-assay. Results Treated with benzyl propionate nandrolone for 48 h,1) G0/G1 phase retardation effect of the cell cycle was relieved(p﹤0.01)when the cell cultured in lower energy, but 2) G2/M block effect of the cell significantly remitted(p﹤0.01)under the condition of high glucose concentration. 3) Benzyl propionate nandrolone can promote Nampt protein expression(p﹤0.05) and promote insulin secretion, but inhibiting the dephosphorylated FOXO1 expression(p﹤0.01). Conclusion Benzyl propionate nandrolone would promote islet NIT-1 cell proliferation and insulin secretion, those effects are closely related to increase Nampt expression inhibit of FOXO1.