贝前列素促进野百合碱诱导肺动脉高压大鼠肺动脉细胞凋亡

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (8): 955-959.

贝前列素促进野百合碱诱导肺动脉高压大鼠肺动脉细胞凋亡

刘亚,田红燕,闫晓丽,王文平,范粉灵

西安交通大学第一附属医院

收稿日期:2012-06-18 修回日期:2012-10-31 出版日期:2013-08-05 发布日期:2013-07-18
通讯作者: 田红燕 E-mail:tianhhyy@163.com
基金资助:
GPCR调节氧敏感性Kv通道机制在肺动脉高压中的作用研究

Beraprosat Promoting the Apoptosis of Pulmonary Artery in the Pulmonary Artery Hypertension Rats Induced by Monocrotaline

Received:2012-06-18 Revised:2012-10-31 Online:2013-08-05 Published:2013-07-18

摘要/Abstract

摘要： 目的探讨前列环素对肺动脉细胞凋亡的影响。方法 30只大鼠，随机分为对照组、野百合碱（MCT）组及贝前列素组。MCT组及贝前列素组腹腔注射60 mg/kg MCT，对照组腹腔注射等体积生理盐水；第2天起，贝前列素组每天200mg /kg贝前列素灌胃。所有大鼠饲养21d后，观察肺动脉血流动力学及右心肥厚、肺组织HE和TUNEL染色分别观察肺动脉重塑和肺动脉细胞凋亡，实时定量PCR及Western blot观察肺动脉内Bax，Bcl-2和PDKmRNA和蛋白水平的改变。结果贝前列素组大鼠的平均肺动脉压力，右心肥厚指数及肺动脉管壁厚度均明显低于MCT组（P<0.05）。贝前列素组大鼠肺动脉细胞凋亡较MCT组增加，肺动脉组织中Bax mRNA和蛋白水平均高于MCT组，而Bcl-2与PDK mRNA和蛋白水平均显著降低。结论贝前列素可以通过促进肺动脉细胞凋亡改善野百合碱诱导肺动脉高压大鼠的肺动脉重塑。

关键词: 凋亡, 肺动脉高压, 贝前列素

Abstract: Objective To investigate the effect of prostacyclin on apoptosis of pulmonary artery. Methods The pulmonary artery hypertension rats were induced by monocrotaline (MCT) intraperitoneal injection. In MCT and beraprosat group, each rat was only given an intraperitoneal injection of 60 mg/kg of MCT. In control group, each rat was only given an intraperitoneal injection of equivalent volume saline. The rats in beraprosat group were given an intragastric administration of 200mg /kg of beraprosat daily from the second day. The hemodynamics, right ventricular hypertrophy, pulmonary artery remodeling, pulmonary artery apoptosis and the molecules involved in apoptosis of PAH were observed. Results In beraprosat group, rats were with lower hemodynamics and right ventricular hypertrophy than MCT group. Beraprosat significantly improved the pulmonary artery remodeling and increased the apoptosis in pulmonary artery. Beraprosat also increased the Bax, decreased the Bcl-2 and PDK in both mRNA and protein level.Conclusions Beraprosat can improve pulmonary artery remodeling by inducing the apoptosis of pulmonary artery.

Key words: Apoptosis, Pulmonary artery Hypertension, Beraprosat

中图分类号:

R 543.2

刘亚田红燕闫晓丽王文平范粉灵. 贝前列素促进野百合碱诱导肺动脉高压大鼠肺动脉细胞凋亡[J]. 基础医学与临床, 2013, 33(8): 955-959.

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