基础医学与临床 ›› 2012, Vol. 32 ›› Issue (7): 793-797.

• 研究论文 • 上一篇    下一篇

MDA-7/IL-24对乳腺癌MDA-MB-231细胞生长抑制和凋亡的影响

李正祎1,王云东郭素红1,姜晓明1,庄文越1   

  • 收稿日期:2011-07-06 修回日期:2011-11-14 出版日期:2012-07-05 发布日期:2012-06-20
  • 通讯作者: 庄文越 E-mail:wenyuezhuang@163.com
  • 基金资助:
    吉林医药学院青年科研基金

MDA-7/IL-24 inhibits the growth and induces the apoptosis of human breast cancer MDA-MB-231 cell line

  • Received:2011-07-06 Revised:2011-11-14 Online:2012-07-05 Published:2012-06-20

摘要: 摘要:目的 探讨IL-24基因转染乳腺癌MDA-MB-231细胞的增殖抑制和促凋亡作用。方法 利用脂质体将穿梭质粒pDC316-hIL-24-EGFP瞬时转染至乳腺癌MDA-MB-231细胞,通过RT-PCR检测转染后hIL-24基因mRNA的转录,Western blot检测转染后IL-24和Caspase-3蛋白的表达,MTT比色法测定细胞增殖的抑制, 流式细胞仪检测细胞的凋亡和细胞周期的变化,Hoechst 33258染色检测细胞的凋亡情况。结果IL-24基因可在MDA-MB-231细胞中成功转录及表达。IL-24可上调MDA-MB-231细胞中Caspase-3蛋白表达。IL-24基因的表达使得乳腺癌MDA-MB-231细胞出现增殖抑制。流式细胞仪检测显示MDA-MB-231细胞DNA 合成受到抑制,细胞周期主要抑制在G2/M期。Hoechst 33258染色显示IL-24基因转染后MDA-MB-231细胞出现凋亡。结论IL-24基因转染乳腺癌MDA-MB-231细胞后能够抑制细胞增殖并促进其凋亡,其机制之一可能是上调Caspase-3的表达。

关键词: IL-24基因, 乳腺癌, 转染, 凋亡

Abstract: Abstract:Objective To explore the effect of IL-24 gene on proliferation and apoptosis of human breast cancer cell line MDA-MB-231. Methods The recombinant shuttle vector pDC316-hIL-24-EGFP was transiently transfected into human breast cancer cell line MDA-MB-231 with lipofectamine. Both IL-24 mRNA and protein were detected by RT-PCR and Western blot respectively.The inhibition of cell proliferation was evaluated by MTT assay. Changes in cell cycle were detected by flow cytometry(FCM) .The cell apoptosis was studied by Hoechst 33258 staining. Results IL-24 was proved successfully transcribed and expressed in MDA-MB-231 cells. IL-24 enhanced protein Caspase-3 expression level of MDA-MB-231cells. The IL-24 expression inhibited MDA-MB-231 cells proliferation. In MDA-MB-231cells of IL-24 over-expressed the DNA synthesis was inhibited and arrested in G2/ M phrase according to FCM.Increased apoptosis of MDA-MB-231 cells were observed by Hoechst33258 staining.Conclusion IL-24 gene overexpressed was able to effectively inhibit the proliferation and promote the apoptosis of breast cancer cells,which may be due to enhancing of protein Caspase-3 leve.

Key words: IL-24 gene, Breast cancer, Transfection, Apoptosis

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