Slug对照射鼠肠上皮细胞的保护作用及机制

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (2): 138-142.

Slug对照射鼠肠上皮细胞的保护作用及机制

迟绍云

山东省海阳市人民医院

收稿日期:2011-09-07 修回日期:2011-12-23 出版日期:2012-02-05 发布日期:2012-01-12
通讯作者: 迟绍云 E-mail:hysymyy@126.com

The protection effect and mechanism of slug on irradiation of rat intestinal epithelial cells

Received:2011-09-07 Revised:2011-12-23 Online:2012-02-05 Published:2012-01-12

摘要/Abstract

摘要： 目的：研究Slug过表达对照射鼠肠上皮细胞的保护作用及机制方法：以体外培养的小肠隐窝上皮细胞IEC6和转染了Slug cDNA的IEC6为研究对象，对细胞进行6Gy剂量γ射线照射，在照射48h后观察细胞凋亡指数，免疫组化和Western blot法检测PUMA蛋白表达。结果：6Gy射线照射IEC6细胞48h后的细胞凋亡指数为15.6+1.54，明显高于照射联合pcDNA3-Slug cDNA转染组的5.1+1.7（P<0.01）和单独pcDNA3-Slug cDNA转染组的2.21+0.36（P<0.01）。Weatern blot法检测发现，6Gy射线照射IEC6细胞48h后的PUMA相对表达为0.79+0.12，明显高于照射联合pcDNA3-Slug cDNA转染组的0.16+0.01（P<0.01），免疫组化和Weatern blot法检测结果一致。结论：上调Slug抑制射线诱导的细胞PUMA表达上调，抑制IEC6细胞凋亡。

关键词: Slug, PUMA, 射线照射, 凋亡, 鼠肠上皮细胞

Abstract: Objective:To investigate the protection effect and mechanism of slug overexpression on irradiation of rat intestinal epithelial cells . Methods: The study are the intestinal crypt epithelial cells (IEC6) and the IEC6 transfected with Slug cDNA in vitro.The cells are irradiated with 6 Gy60Co gamma rays.48 hours after the irradiation, Apoptosis indexes are calculated.The expression of PUMA are observed by the Immunohistochemistry and Western-blot.Result: The apoptosis indexes in IEC6 by the irradiation of 60Co gamma rays after 48 hours is significantly higher than in pcDNA3-Slug cDNA and in pcDNA3-Slug cDNA without irradiation(15.6+1.54 versus 5.1+1.7; 2.21+0.36; P<0.01). The expression of PUMA in IEC6 by the irradiation of 60Co gamma rays after 48 hours is significantly higher than in pcDNA3-Slug cDNA by Western-blot (0.79+0.12 versus 0.16+0.01; P<0.01). The Immunohistochemistry and Weatern blot assay results are consistent.Conclusions: Upregulation of Slug can restrain the upregulation expression of PUMA in irradiated cells and the apoptosis of IEC6.

Key words: Slug, PUMA, Ray irradiation, Apoptosis, Rat intestinal epithelial cells

迟绍云. Slug对照射鼠肠上皮细胞的保护作用及机制[J]. 基础医学与临床, 2012, 32(2): 138-142.

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