厄罗替尼联合塞来昔布阻断EGFR和COX-2抑制肺癌A549细胞增殖

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (1): 19-24.

厄罗替尼联合塞来昔布阻断EGFR和COX-2抑制肺癌A549细胞增殖

白小燕¹,牟晓燕²,姜淑娟²,王亚丽²,刘庆亮²

1. 山东大学附属省立医院呼吸科2. 山东大学附属省立医院

收稿日期:2009-11-17 修回日期:2010-05-04 出版日期:2011-01-05 发布日期:2011-01-05
通讯作者: 牟晓燕

Erlotinib combined with Celecoxib inhibits the growth of Lung Cancer A549 cells by Simultaneously blocking EGFR and COX-2

BAI Xiao-yan ¹,MU Xiao-yan ¹,JIANG Shu-juan ²,WANG Ya-li ²,LIU Qing-liang ²

1. Provincial Hospital Affiliated to Shandong University2.

Received:2009-11-17 Revised:2010-05-04 Online:2011-01-05 Published:2011-01-05
Contact: MU Xiao-yan

摘要/Abstract

摘要： 目的探讨厄罗替尼联合塞来昔布对肺腺癌A549细胞株凋亡和EGFR、COX-2表达的影响。方法厄罗替尼、塞来昔布单独或联合干预细胞48h后，倒置相差显微镜观察细胞形态；四甲基偶氮唑盐（MTT）测定细胞抑制率；Hoechst33258法和TUNEL法检测细胞凋亡和流式细胞术检测细胞周期；免疫荧光检测EGFR和COX-2蛋白表达。结果厄罗替尼联合塞来昔布，相比单药组A549细胞明显出现大量颗粒和空泡，细胞变圆开始脱落；二者联合作用时抑制作用更强（P<0．05)，厄罗替尼与塞来昔布均能诱导细胞凋亡，联合作用后细胞凋亡率更高（P<0．05),并使细胞发生明显的G1期阻滞（P<0．05)，进一步下调了EGFR和COX-2蛋白的表达（P<0．05)。结论厄罗替尼与塞来昔布联合应用具有协同介导细胞凋亡，阻滞细胞周期于G0/G1期及阻滞EGFR和COX-2信号途径有关。

关键词: 厄罗替尼, 塞来昔布, A549细胞, 凋亡, 表皮生长因子受体, 环氧合酶-2

Abstract: Objective To explore the effects of Erlotinib combined with Celecoxib on apoptosis and expression of EGFR and COX-2 in lung cancer A549 cells.Methods A549 cells were observed under an inverted microscope ,MTT was used to measure the growth inhibitory effects of A549 cells by Erlotinib and Celecoxib．The cell apoptosis was studied byTUNEL and Hoechst33258 staining method . The cell cycle was detected by flow cytometer,and the expression of EGFR,COX-2 were determined by immunofluorescence. Results A great quantity of granules and vacuolus were observed in the combined treatment A549 ,with cell rounding and defluxion（P<0．05).The combination increased apoptosis of A549 (P<0．05) and significantly induced G1 phase arrest (P<0．05)and down-regulated expression of EGFR,COX-2 in A549 cells(P<0．05). Conclusions The results suggest that combination treatment with Erlotinib and Celecoxib showed significant synergistic inducing apoptosis and G1 phase arrest ，owing to down-regulating expressing both EGFR and COX-2．

白小燕;牟晓燕;姜淑娟;王亚丽;刘庆亮. 厄罗替尼联合塞来昔布阻断EGFR和COX-2抑制肺癌A549细胞增殖[J]. 基础医学与临床, 2011, 31(1): 19-24.

BAI Xiao-yan ;MU Xiao-yan ;JIANG Shu-juan ;WANG Ya-li ;LIU Qing-liang . Erlotinib combined with Celecoxib inhibits the growth of Lung Cancer A549 cells by Simultaneously blocking EGFR and COX-2[J]. Basic & Clinical Medicine, 2011, 31(1): 19-24.

[1]	刘雯霞刘彩红郭蕊孟芝君高佳王亚静. 尼古丁促进高糖/高脂培养的大鼠心肌细胞系H9C2凋亡[J]. , 2020, 40(1): 24-29.
[2]	蒋庆锋于永魁程金华申思宁邢文群. lncRNA HEIH通过miR-98-5p调节肺癌细胞增殖和凋亡[J]. , 2020, 40(1): 30-36.
[3]	苗玉娟王淑坤孙丹丁康刘美逸张妍周风华. miR-181 通过 Prox1 促进人乳腺癌细胞系MCF-7的增殖并抑制凋亡[J]. 基础医学与临床, 2019, 39(9): 1265-1269.
[4]	童静凯江霞. 托伐普坦对小鼠内髓集合管细胞系水通道蛋白及肾小管功能的影响[J]. 基础医学与临床, 2019, 39(8): 1163-1167.
[5]	晋瑞金迎迎李嫚雷喆. 过表达脾酪氨酸激酶降低氧化应激诱导的小鼠成骨细胞凋亡[J]. 基础医学与临床, 2019, 39(7): 1013-1018.
[6]	任玮余宏川王萍刘英茹. miR-17-5p诱导小鼠肾足细胞系凋亡[J]. 基础医学与临床, 2019, 39(7): 973-977.
[7]	雷晓燕孙永红陈星星崔维静刘璟. 乙肝病毒X蛋白抑制小鼠足细胞系MPC5增殖并促进其凋亡[J]. 基础医学与临床, 2019, 39(5): 617-622.
[8]	马从乾王雅王娜杨柯陈德才. miR-181a过表达加重H2O2诱导的HUVECs损伤[J]. 基础医学与临床, 2019, 39(5): 690-695.
[9]	王圣凯胡苏琴吴玲吴杭菲郑靖宇蒋自培李剑敏. miR-138在乙醇诱导的大鼠心肌细胞系H9C2氧化应激中表达增加[J]. 基础医学与临床, 2019, 39(5): 677-681.
[10]	战仕胜李军杨婷婷王雅蓉王秀青. 抗菌肽merecidin诱导人肺腺癌细胞系A549凋亡[J]. 基础医学与临床, 2019, 39(4): 473-477.
[11]	刘莹张艳丽王秀芳. 沉默FOXO1抑制H2O2诱导的大鼠肺泡2型上皮细胞凋亡[J]. 基础医学与临床, 2019, 39(4): 519-523.
[12]	张娟冯燕和水祥. 过表达miR-5195-3p对结直肠癌细胞凋亡的影响[J]. 基础医学与临床, 2019, 39(3): 385-391.
[13]	柴华金翔宇金霞云杨兴无. 微卫星不稳定、HER2与结直肠癌恶性程度相关[J]. 基础医学与临床, 2019, 39(2): 218-221.
[14]	张许梅王瑾霍海燕岳继萍张文婷焦向英. 血管紧张素II 1型受体自身抗体可上调TXNIP导致大鼠胰岛β细胞系INS-1凋亡[J]. , 2019, 39(11): 1548-1555.
[15]	宁娜侯晓鸿李杨何金玲燕子王晓晖王丽. 自噬降低激活β1-AA诱导的心肌细胞内质网应激相关凋亡途径[J]. 基础医学与临床, 2019, 39(1): 1-6.