miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells

doi:10.16352/j.issn.1001-6325.2025.11.1457

Abstract

Abstract: Objective To explore the protective effect of miR-374c-5p on hydrogen peroxide (H₂O₂) -induced apoptosis of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro and the harvested cells were divided into four groups: control group, H₂O₂ group, H₂O₂ + miR-374c-5p mimics transfection group, and H₂O₂ + miR-374c-5p inhibitor transfection group. Cell activity was assessed by CCK-8 proliferation rate assay, apoptosis was detected by TUNEL staining microscopy. Expression of miR-374c-5p and Fas mRNA by RT-qPCR, and Fas protein in HUVECs by was detected by Western blot. Results Proliferation of HUVECs was significantly inhibited(P＜0.001);H₂O₂ was significantly increased as compared with the H₂O₂ intervention group(P＜0.001);Proliferation in H₂O₂ + miR-374c-5p inhibitor transfection group was significantly increased as compared to H₂O₂ intervention group(P＜0.001). Conclusions miR-374c-5p protectes the HUVECs against apoptosis induced by H₂O₂.

Key words: miR-374c-5p, human umbilical vein endothelial cells(HUVECs), hydrogen peroxide(H₂O₂), oxidative stress, apoptosis

CLC Number:

JIA Zonghu, JIN Qun, HAN Shufang, HU Yuhong, REN Changzhen, LI Yunping, LIU Wenyan. miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells[J]. Basic & Clinical Medicine, 2025, 45(11): 1457-1462.

References 13

[1]	Stanton T, Haluska BA, Leano R, et al. Hemodynamic benefit of rest and exercise optimization of cardiac resynchronization therapy[J]. Echocardiography, 2014:31980-31988.
[2]	Olah A, Nemeth BT, Matyas C, et al. Cardiac effects of acute exhaustive exercise in a rat model[J]. Int J Cardiol, 2015,1:182:258-266.
[3]	Shi M, Dong Z, Zhao K,et al. Novel insights into exhaustive exercise-induced myocardial injury: focusing on mitochondrial quality control[J]. Front Cardiovasc Med, 2022,14,9:1015639.doi:10.3389/fcvm.2022.1015639.
[4]	Loboda A, Damulewicz M, Pyza E, et al. Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism[J]. Cell Mol Life Sci, 2016,73:3221-3247.
[5]	Zhang XD, Liu ZY, Wang MS, et al. Mechanisms and regulations of ferroptosis[J]. Front Immunol, 2023,14:1269451.doi:10.3389/fimmu.2023.1269451.
[6]	宋颖, 李嘉颖, 殷敏.等. 耐力训练对小鼠力竭运动后心肌组织中circRNA-lncRNA-miRNA-mRNA表达谱的影响[J]. 海南医学院学报,2023,29:175-182.
[7]	Pang QQ, Kim JH, Kim HY, et al. Protective effects and mechanisms of pectolinarin against H2O2-induced oxidative stress in SH-SY5Y neuronal cells[J]. Molecules, 2023,28:5826.doi:10.3390/molecules28155826.doi:10.3390/molecules28155826.
[8]	Zhang HM, LIU MY, Zhang YY, et al. Trimetazidine attenuates exhaustive exercise-induced myocardial injury in rats via regulation of the Nrf2/NF-κB signaling pathway[J]. Front Pharmacol,2019,10:175.doi:10.3389/fphar.2019.00175.doi:10.3389/fphar.2019.00175.
[9]	Wodarski A, Strycharz J, Wróblewski A,et al. The role of microRNAs in metabolic syndrome-related oxidative stress[J]. Int J Mol Sci, 2020,21:6902.doi:10.3390/ijms21186902.
[10]	Ait-Aissa K, Nguyen QM, Gabani M, et al. MicroRNAs and obesity-induced endothelial dysfunction: key para-digms in molecular therapy[J]. Cardiovasc Diabetol, 2020,19:136.doi:10.1186/s12933-020-01107-3.
[11]	Xun M, Zhang J, Wu M, et al. Long non-coding RNAs: The growth controller of vascular smooth muscle cells in cardiovascular diseases[J].Int J Biochem Cell Biol, 2023,157:106392.doi:10.1016/j.biocel.2023.106392.
[12]	Dong L, Zheng YM, Gao LB, et al. lncRNA NEAT1 prompts autophagy and apoptosis in MPTP-induced Parkinson's disease by impairing miR-374c-5p[J]. Acta Biochim Biophys Sin(Shanghai),2021, 53:870-882.
[13]	Zhao L, Han L, Hei G,et al. Diminished miR-374c-5p negatively regulates IL(interleukin)-6 in unexplained recurrent spontaneous abortion[J]. Mol Med(Berl), 2022,100:1043-1056.

[1]	WANG Aojun, YAO Ruixin, LYU Yue, XU Yu, ZHANG Xiaotian. Heme oxygenase-1 mediates sodium butyrate to alleviate non-alcoholic steatohepatitis [J]. Basic & Clinical Medicine, 2026, 46(1): 85-91.
[2]	CHEN Xing, LI Yulong, ZHANG Bing, LIANG Jinjie, CHEN Ziyi. Polyene phosphatidylcholine repairs high glucose-induced human umbilical vein endothelial cells damage [J]. Basic & Clinical Medicine, 2026, 46(1): 71-77.
[3]	LI Na, ZHANG Peng, ZHANG Nan, LIN Wei, LIU Defeng, ZHENG Jihui. Scutellarin reduces apoptosis of nucleus pulposus cells in intervertebral disc degeneration of rat models [J]. Basic & Clinical Medicine, 2025, 45(9): 1158-1164.
[4]	YU Huahui, DU Zhiyong, JIAO Xiaolu, LYU Qianwen, YANG Yunyun, HAN Lijie, QIN Yanwen. Angiopoietin-like protein 8 promotes apoptosis of vascular smooth muscle cells through activating p53 signaling pathway [J]. Basic & Clinical Medicine, 2025, 45(7): 882-888.
[5]	SUN Ting, ZOU Teng, YANG Yisong, LIU Shuangping, REN Xin, WANG Dan. Inhibitory effect of β-elemonic acid on proliferation and invasion of colon cancer cell lines [J]. Basic & Clinical Medicine, 2025, 45(6): 748-754.
[6]	ZHANG Fangling, ZHANG Yuanfang, ZHANG Li. Dexmedetomidine alleviates nerve injury induced by propofol in neonatal rats [J]. Basic & Clinical Medicine, 2025, 45(5): 644-650.
[7]	YIN Lijun, BO Yang, Anggelema, SONG Linlin. LncRNA HIF1A-AS1 promotes the proliferation of gastric cancer cell lines through interfering with HIF1A [J]. Basic & Clinical Medicine, 2025, 45(5): 627-636.
[8]	WEI Jianxin, FANG Yanle, LU Yubo, GAO Yuguang, LANG Xing, LI Jingtao, MA Xinsheng. Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 [J]. Basic & Clinical Medicine, 2025, 45(4): 493-498.
[9]	WANG Pei, BIAN Tao, WU Yan. Acetylation modification and chronic obstructive pulmonary disease [J]. Basic & Clinical Medicine, 2025, 45(4): 536-541.
[10]	LIU Genfeng, NAN Lu, GAO Qin, CHEN Yixuan, ZHANG Jing, YU Peng, YU Shuchun. Dexmedetomidine alleviatesmyocardial ischemia-reperfusion injury in rat models [J]. Basic & Clinical Medicine, 2025, 45(3): 303-309.
[11]	LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun. Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292 [J]. Basic & Clinical Medicine, 2025, 45(3): 336-340.
[12]	WEN Wen, LIU Chenxi, CHEN Shuangjing, LU Xiaojiong, JIN Zhitao, ZHANG Zheng. Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury [J]. Basic & Clinical Medicine, 2025, 45(12): 1557-1564.
[13]	CHENG Yongxia, YU Long, LI Huamin, ZHAO Shuo, ZHANG Yiyang, LIU Guibo. Knockdown of PIAS3 alleviates glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in rat cardiomyocyte cell line H9c2 [J]. Basic & Clinical Medicine, 2025, 45(12): 1593-1599.
[14]	LI Haipeng, LIU Yifan, LI Shan, CHEN Daohui, LEI Jinglin, HU Zheng. Elevated expression of miR-423-5p in the plasma of patients with ischemic stroke [J]. Basic & Clinical Medicine, 2025, 45(12): 1572-1579.
[15]	LI Wenhua, WANG Runlin, KANG Qianpeng, HUANG Mei, GUO Zhengguang, ZHANG Chunlin, HUANG Yongsheng. p97 inhibitor EerⅠ induces apoptosis and ferroptosis of gastric cancer cell line AGS [J]. Basic & Clinical Medicine, 2025, 45(11): 1401-1408.