miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells

doi:10.16352/j.issn.1001-6325.2025.11.1457

Abstract

Abstract: Objective To explore the protective effect of miR-374c-5p on hydrogen peroxide (H₂O₂) -induced apoptosis of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro and the harvested cells were divided into four groups: control group, H₂O₂ group, H₂O₂ + miR-374c-5p mimics transfection group, and H₂O₂ + miR-374c-5p inhibitor transfection group. Cell activity was assessed by CCK-8 proliferation rate assay, apoptosis was detected by TUNEL staining microscopy. Expression of miR-374c-5p and Fas mRNA by RT-qPCR, and Fas protein in HUVECs by was detected by Western blot. Results Proliferation of HUVECs was significantly inhibited(P＜0.001);H₂O₂ was significantly increased as compared with the H₂O₂ intervention group(P＜0.001);Proliferation in H₂O₂ + miR-374c-5p inhibitor transfection group was significantly increased as compared to H₂O₂ intervention group(P＜0.001). Conclusions miR-374c-5p protectes the HUVECs against apoptosis induced by H₂O₂.

Key words: miR-374c-5p, human umbilical vein endothelial cells(HUVECs), hydrogen peroxide(H₂O₂), oxidative stress, apoptosis

CLC Number:

JIA Zonghu, JIN Qun, HAN Shufang, HU Yuhong, REN Changzhen, LI Yunping, LIU Wenyan. miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells[J]. Basic & Clinical Medicine, 2025, 45(11): 1457-1462.

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