Abstract: Objective To explore the impact of celastrol on polarization of mouse peritoneal macrophage. Methods Mouse peritoneal macrophages were isolated by intraperitoneal injection of broth (stimulation method). Cells were divided into control group, celastrol only group, classically activated M1 group and celastrol intervention with M1 group. RT-qPCR and flow cytometry were used to detect the relevant markers expression levels of two polarization states of macrophages (M1 macrophages and M2 macrophages)——iNOS, Arg-1, CD206, CD11c. Results Celastrol can decrease the expressions of revelant markers CD11c and iNOS of M1 macrophages (P<0.05). Celastrol can enhance the expressions of revelant marker Arg-1 of M2 macrophages (P<0.05). Conclusions Celastrol can suppress the M1 macrophage polarization of mouse peritoneal macrophages.

Key words: Key words: celastrol, macrophage