Effects of ANGPT1 on proliferation,invasion and angiogenesis of mouse prostate cancer cell line RM-1

doi:10.16352/j.issn.1001-6325.2025.09.1165

Abstract

Abstract: Objective To explore the regulatory mechanism of angiopoietin 1 (ANGPT1) on proliferation, invasion and angiogenesis of prostate cancer cells. Methods Mouse prostate cancer cell line (RM-1) was divided into control group, NC-oe group, ANGPT1-oe group, NC-sh group and ANGPT1-sh group. NC-oe, ANGPT1-oe, NC-sh and ANGPT1-sh were transfected into RM-1 cells by Lipofectamine3000 reagent. The transfection efficiency was verified by RT-qPCR and Western blot, cell proliferation was detected by MTT assay and EdU staining and cell invasion was detected by Transwell assay. The cells were divided into the following groups: control group, Tie2-expressing monocytes/macrophages(TEMs) group, NC-oe+TEM group, ANGPT1-oe+TEM group, NC-sh+TEM group and ANGPT1-sh+TEM groups. RM-1 and TEM were co-cultured. RM-1 cells were collected after 72 hours and subjected to MTT proliferation assay, EdU staining assay and Transwell invasion assay. The co-cultured cell supernatant from each group mouse umbilical vein endothelial cells (MUVECs) were co-incubated with cell supernatant collected from each group cells and then to detect the number of tubules formed. The co-cultured cell supernatant of each group was collected, and the level of MMP-9, VEGFA and COX-2 were detected by ELISA. Results 1)Compared with control group and NC-oe group, the level of ANGPT1 mRNA and protein in ANGPT1-oe group was increased (P＜0.05). Compared with control group and NC-sh group, the level of ANGPT1 mRNA and protein in ANGPT1-sh group was decreased (P＜0.05). 2) Compared with control group, the cell viability, EdU positive rate, counting of invasive cells and of tubules formed in TEM group significantly increased(P＜0.05), the level of MMP-9, VEGFA and COX-2 in the supernatant was increased (P＜0.05). Compared with TEM group and NC-oe+TEM group, the cell viability, EdU positive rate, counting of invasive cells and of tubules formed in ANGPT1-oe+TEM group decreased (P＜0.05).The level of MMP-9, VEGFA and COX-2 in the supernatant was significantly decreased (P＜0.05). Compared with TEM group and NC-sh+TEM group, the cell viability, EdU positive rate, counting of invasive cell and of tubules formed in ANGPT1-sh+TEM group all increased (P＜0.05).The level of MMP-9, VEGFA and COX-2 in the supernatant were increased(P＜0.05). Conclusions The decreased expression of ANGPT1 in prostate cancer significantly enhances the promotion effect of TEMs on the proliferation, invasion and angiogenesis of prostate cancer cells, thus promoting the progression of prostate cancer.

Key words: prostate cancer, angiopoietin 1, proliferation, invasion, angiogenesis

CLC Number:

AN Xin, JIA Lin, Mawusumu·MAMUT, HUANG Zhipeng, KahrimanI·SLAM, Rexiati·REHEMAN, Hairila·WULAMU, Kerbanjiang·ABULIKIM, Jesur·BATUR. Effects of ANGPT1 on proliferation,invasion and angiogenesis of mouse prostate cancer cell line RM-1[J]. Basic & Clinical Medicine, 2025, 45(9): 1165-1172.

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