Gypenoside inhibits the proliferation, migration and invasion of prostate cancer cell line PC-3

doi:10.16352/j.issn.1001-6325.2025.08.1059

Abstract

Abstract: Objective To investigate the effects of gypenoside(Gyp) on proliferation, migration and invasion of human prostate cancer cell line PC-3. Methods PC-3 cells were treated with 200-1 200 μg/mL Gyp to determine the optimal concentration. PC-3 cells were divided into control, Gyp-L group(400 μg/mL), Gyp-M group(600 μg/mL) and Gyp-H group(800 μg/mL), and Gyp-H+8-bromo-cAMP group(100 μmol/L PKA activator). Colony formation assay was applied to detect cell proliferation. Apoptosis was measured by flow cytometry. Scratch experiment was applied to detect the migration ability. Transwell assay was applied to detect the invasive ability. ELISA experiment was applied to detect cAMP level in each group. Western blot was applied to detect the expression of E-cadherin, N-cadherin, Snail, PKA and CREB proteins. Results Gyp inhibited PC-3 proliferation in a concentration dependent manner. Gyp concentration of 400, 600 and 800 μg/mL was selected for subsequent experiments. Compared with the control group, colony formation, number of invasive cells, scratch healing rate, the levels of Snail,N-cadherin, PKA, cAMP, and CREB proteins in the Gyp-L, Gyp-M, and Gyp-H groups were significantly reduced (P＜0.05),but the apoptosis rate as well as the level of E-cadherin protein were significantly increased(P＜0.05). PKA activator attenuated the inhibitory effect of Gyp on the malignant behavior of prostate cancer cells. Conclusions Gyp inhibits proliferation, migration and invasion, and promotes apoptosis in prostate cancer cell line PC-3.

Key words: gypenoside, prostate cancer, proliferation, migration

CLC Number:

R737.25

ZOU Qingbo, ZHANG Guochen, PAN Changjing. Gypenoside inhibits the proliferation, migration and invasion of prostate cancer cell line PC-3[J]. Basic & Clinical Medicine, 2025, 45(8): 1059-1065.

References

[1] 侯佩先, 金坤, 邱实, 等. 中性粒细胞和淋巴细胞比值对根治性前列腺切除术患者生化复发的影响[J]. 现代泌尿外科杂志, 2020, 25:392-396.
[2] 张钰菲, 任正举, 曹德宏, 等. 前列腺“炎－癌转化”的研究进展[J]. 现代泌尿外科杂志, 2020, 25:453-456.
[3] Hsu HY, Yang JS, Lu KW, et al. An experimental study on the antileukemia effects of gypenosides in vitro and in vivo[J]. Integr Cancer Ther, 2011, 10:101-112.
[4] Li X, Liu H, Lv C, et al. Gypenoside-induced apoptosis via the PI3K/AKT/mTOR signaling pathway in bladder cancer[J]. Biomed Res Int, 2022, 291:9304552-9304567.
[5] Hu S, Wang L, Zhang X, et al. Autophagy induces transforming growth factor-β-dependent epithelial-mesenchymal transition in hepatocarcinoma cells through cAMP response element binding signalling[J]. J Cell Mol Med, 2018, 22:5518-5532.
[6] Haffner MC, Zwart W, Roudier MP, et al. Genomic and phenotypic heterogeneity in prostate cancer[J]. Nat Rev Urol, 2021, 18:79-92.
[7] Zhang HK, Ye Y, Li KJ, et al. Gypenosides prevent H2O2-induced retinal ganglion cell apoptosis by concurrently suppressing the neuronal oxidative stress and inflammatory response[J]. J Mol Neuro, 2020, 70:618-630.
[8] 姜秀芳, 耿亚楠, 阳盛洪, 等. 绞股蓝皂苷减轻脂多糖诱导大鼠肾上腺嗜铬细胞瘤细胞系PC12损伤[J]. 基础医学与临床, 2022, 42:908-912.
[9] Shi L, Pi Y, Luo C, et al. In vitro inhibitory activities of six gypenosides on human liver cancer cell line HepG2 and possible role of HIF-1α pathway in them[J]. Chem Biol Interact, 2015, 238:48-54.
[10] Xing SF, Liu LH, Zu ML, et al. The inhibitory effect of gypenoside stereoisomers, gypenoside L and gypenoside LI, isolated from Gynostemma pentaphyllum on the growth of human lung cancer A549 cells[J]. J Ethnopharmacol, 2018, 219:161-172.
[11] Fang C, Kang Y. E-Cadherin: context-dependent functions of a quintessential epithelial marker in metastasis[J]. Cancer Res, 2021, 81:5800-5802.
[12] Kielbik M, Szulc-Kielbik I, Klink M. Impact of selected signaling proteins on SNAIL 1 and SNAIL 2 expression in ovarian cancer cell lines in relation to cells' cisplatin resistance and EMT markers level[J]. Int J Mol Sci, 2021, 22:980-992.
[13] Wu J, Gan Y, Luo H, et al. β-Patchoulene ameliorates water transport and the mucus barrier in 5-fluorouracil-induced intestinal mucositis rats via the cAMP/PKA/CREB signaling pathway[J]. Front Pharmacol, 2021, 12:689491-689503.
[14] Li SY, Shang J, Mao XM, et al. Diosgenin exerts anti-tumor effects through inactivation of cAMP/PKA/CREB signaling pathway in colorectal cancer[J]. Eur J Pharmacol, 2021, 908:174370-174384.
[15] Zhang H, Yang S, Wang J, et al. Blockade of AMPK-mediated cAMP-PKA-CREB/ATF1 signaling synergizes with aspirin to inhibit hepatocellular carcinoma[J]. Cancers, 2021, 13:1738-1758.