NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B

doi:10.16352/j.issn.1001-6325.2025.08.1010

Abstract

Abstract: Objective To investigate the mechanism of reactive oxygen species (ROS) and pyroptosis in amiodarone-induced lung epithelial cell injury. Methods The viability of cells treated with amiodarone, amiodarone+N-acetylcysteine(ROS inhibitor,NAC) and amiodarone+NF-κB inhibitors was detected by MTT assay. ROS level was detected by DCFA probe and the levels of IL-6, IL-18, IL-1β and TNF-α in the supernatant were detected by ELISA; NLRP3. Caspase-1 p20 and GSDMD-N protein were detected by Western blot. Results Amiodarone concentration-dependently reduced cell viability(P＜0.05), NAC and NF-κB attenuated the cytotoxicity of amiodarone(P＜0.05). Amiodarone increased levels of reactive oxygen species, inflammatory factors and pyroptosis-related proteins in lung epithelial cells(P＜0.05), while NAC and NF-κB inhibitors reduced the levels of these indicators(P＜0.05). Conclusions NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line in vitro.

Key words: amiodarone, reactive oxygen species, cell injury, NF-κB

CLC Number:

JI Zezhao, ZHANG Qi, Abuduxukuer·ABULIMITI. NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B[J]. Basic & Clinical Medicine, 2025, 45(8): 1010-1015.

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