Knockdown of lncRNA UCA1 reduces gemcitabine resistance of human bladder cancer cell line T24

doi:10.16352/j.issn.1001-6325.2024.08.1113

Abstract

Abstract: Objective To investigate the in vitro effect of lncRNA UCA1 on gemcitabine (GEM) resistance of bladder cancer cell line T24 and its related molecular mechanism. Methods The mRNA expression of UCA1 in T24 cells and in T24/GEM cells was detected by RT-qPCR. The T24/GEM cells were incubated with varying concentrations (0.1, 1, and 10 μmol/L) of GEM for 48 hrs. LC3 staining microscopy was employed to visualize autophagic puncta, while the expression of autophagy-related proteins was assessed by Western blot. UCA1-shRNA and UCA1-shRNA+ pcDNA-Bcl-2 were transferred into T24/GEM cells, the sensitivity of cells to GEM was evaluated by MTT method and flow cytometry; the expressions of p53 and Bcl-2 were detected by Western blot. Results The expression level of UCA1 in T24/GEM cells was significantly higher than that of parental T24 cells (P＜0.05). The concentration of GEM in the range of 0-10 μmol/L significantly induced dose-dependent autophagy in T24/GEM cells (P＜0.05). Knockdown of UCA1 enhanced the sensitivity of T24/GEM cells to GEM (P＜0.05), while reducing autophagy (P＜0.05) and down-regulating the expression of p53 and Bcl-2(P＜0.05). Over-expression of Bcl-2 partially reversed the GEM sensitization and autophagy inhibition of UCA1-shRNA in T24/GEM cells (P＜0.05). Conclusions Knockdown of lncRNA UCA1 reduces GEM resistance of T24/GEM cells by inhibiting Bcl-2 mediated autophagy.

Key words: bladder cancer, lncRNA UCA1, Bcl-2, autophagy, gemcitabine

CLC Number:

R737.1

ZHOU Changdong, LIN Yang, SUN Kai, TIAN Yuxin. Knockdown of lncRNA UCA1 reduces gemcitabine resistance of human bladder cancer cell line T24[J]. Basic & Clinical Medicine, 2024, 44(8): 1113-1119.

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